Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	966,584	G→A	*755* (TAG→TAA)	ycaI →	ComEC family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	966,584	0	G	A	100.0%	224.1 / NA	68	*755* (TAG→TAA)	ycaI	ComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/34);  total (34/34)

TGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAG  >  NC_000913/966450‑966717                                                                                                                                       |                                                                                                                                      tGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAata                                                                                                                                   >  1:1113582/1‑139 (MQ=255)      tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                              <  1:4858/139‑1 (MQ=255)              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAatat                                                                                                                                  <  2:1292250/127‑1 (MQ=255)              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAatat                                                                                                                                  >  1:1292250/1‑127 (MQ=255)              cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTca                                                                                                                      >  2:599856/1‑139 (MQ=255)                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTcaac                                                                                                                    >  2:1441427/1‑139 (MQ=255)                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTcaac                                                                                                                    >  1:859966/1‑139 (MQ=255)                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTc                                                                                                                       <  1:728712/136‑1 (MQ=255)                caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTc                                                                                                                       >  2:728712/1‑136 (MQ=255)                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACaaa                                                                                                                 >  1:447559/1‑139 (MQ=255)                   cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACaaa                                                                                                                 >  1:300330/1‑139 (MQ=255)                     aGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                               <  2:1186765/139‑1 (MQ=255)                        tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGc                                                                                                                             >  1:836594/1‑122 (MQ=255)                        tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGc                                                                                                                             <  2:836594/122‑1 (MQ=255)                        tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTg                                                                                                            >  1:1276981/1‑139 (MQ=255)                        tCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTg                                                                                                            >  1:1294251/1‑139 (MQ=255)                          aaaCAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                          <  1:618386/139‑1 (MQ=255)                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGC‑AATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCt                                                                                                             <  1:1444538/128‑1 (MQ=255)                                 ggTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGC‑AATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCt                                                                                                             >  2:1444538/1‑128 (MQ=255)                                      aTTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCa                                                                                              <  2:1276981/139‑1 (MQ=255)                                             aGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGAc                                                                                       <  2:1113582/139‑1 (MQ=255)                                                gcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                    <  2:1294251/139‑1 (MQ=255)                                                 cAAGGCTGGCGGATTAGGAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGc                                                                                                                             >  1:941312/1‑97 (MQ=255)                                                 cAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCggt                                                                                                                             <  2:941312/97‑2 (MQ=255)                                                  aaGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGa                                                                                  <  1:599856/139‑1 (MQ=255)                                                         gCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATctctct                                                                           <  2:300330/139‑1 (MQ=255)                                                             aTTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGTGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGt                                                                       <  2:859966/139‑1 (MQ=255)                                                                agcagcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGc                                                                    <  1:1441427/139‑1 (MQ=255)                                                                      cTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                              >  1:44940/1‑75 (MQ=255)                                                                      cTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                              <  2:44940/75‑1 (MQ=255)                                                                       tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAg                                                                  >  1:451515/1‑134 (MQ=255)                                                                       tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAg                                                                  <  2:451515/134‑1 (MQ=255)                                                                         aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                              >  1:338777/1‑72 (MQ=255)                                                                         aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCgg                                                                                                                              <  2:338777/72‑1 (MQ=255)                                                                               cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGTGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                  >  2:580950/1‑94 (MQ=255)                                                                               cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGTGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                  <  1:580950/94‑1 (MQ=255)                                                                                  aTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACtgt                                                  >  1:1365020/1‑139 (MQ=255)                                                                                              tGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCt                                                                                                             >  1:851051/1‑68 (MQ=255)                                                                                              tGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCt                                                                                                             <  2:851051/68‑1 (MQ=255)                                                                                                     atcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGTCATTCCGCCGACTGTGGcc                                              >  1:966620/1‑124 (MQ=255)                                                                                                     atcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGcc                                              <  2:966620/124‑1 (MQ=255)                                                                                                      tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                             >  1:1200646/1‑76 (MQ=255)                                                                                                      tcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                             <  2:1200646/76‑1 (MQ=255)                                                                                                         gTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaaa                           <  2:447559/139‑1 (MQ=255)                                                                                                          tGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                          >  2:847114/1‑139 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                                                                                           >  2:498369/1‑69 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                                                                                           <  1:498369/69‑1 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                      <  1:535221/74‑1 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                      >  2:535221/1‑74 (MQ=255)                                                                                                               tGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGt                     >  2:822780/1‑139 (MQ=255)                                                                                                                    tGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgc                                                         <  1:1215198/98‑1 (MQ=255)                                                                                                                    tGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgc                                                         >  2:1215198/1‑98 (MQ=255)                                                                                                                    tGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGAt                >  1:691366/1‑139 (MQ=255)                                                                                                                      ccAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                               >  2:203064/1‑42 (MQ=255)                                                                                                                      ccAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                               <  1:203064/42‑1 (MQ=255)                                                                                                                      ccAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                          <  2:1318194/47‑1 (MQ=255)                                                                                                                      ccAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGt                                                                                                          >  1:1318194/1‑47 (MQ=255)                                                                                                                       cAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                               >  1:415720/1‑41 (MQ=255)                                                                                                                       cAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATg                                                                                                               <  2:415720/41‑1 (MQ=255)                                                                                                                          tGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc          <  2:691366/139‑1 (MQ=255)                                                                                                                           ggATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGa                                                                                  <  1:30181/66‑1 (MQ=255)                                                                                                                           ggATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGa                                                                                  >  2:30181/1‑66 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATctctct                                                                           >  2:725500/1‑67 (MQ=255)                                                                                                                                 cGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATctctct                                                                           <  1:725500/67‑1 (MQ=255)                                                                                                                                  gggTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg  >  1:1369591/1‑139 (MQ=255)                                                                                                                                   ggTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                      <  1:471889/54‑1 (MQ=255)                                                                                                                                   ggTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                      >  2:471889/1‑54 (MQ=255)                                                                                                                                    gTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCCCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt    <  2:1254355/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAG  >  NC_000913/966450‑966717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑