Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,008	G→A	*453* (TAG→TAA)  V3I (GTT→ATT)	murF → mraY →	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,008	0	G	A	100.0%	207.9 / NA	64	*453* (TAG→TAA)  V3I (GTT→ATT)	murF mraY	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/31);  total (33/31)

TGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGA  >  NC_000913/95882‑96134                                                                                                                               |                                                                                                                               tGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCg                                                                                                                    >  2:1352437/1‑139 (MQ=255)       aaTTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTGGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              <  1:506004/139‑1 (MQ=255)       aaTTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              <  1:1352437/139‑1 (MQ=255)                  ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAatat                                                                                                   >  1:720455/1‑139 (MQ=255)                           tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGTCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGc                                                                                          >  2:823673/1‑139 (MQ=255)                           tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              <  2:813662/119‑1 (MQ=255)                           tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              >  1:813662/1‑119 (MQ=255)                           tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGc                                                                                          >  1:823418/1‑139 (MQ=255)                             aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtggc                                                                                                                          >  1:1310310/1‑105 (MQ=255)                             aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTtggc                                                                                                                          <  2:1310310/105‑1 (MQ=255)                             aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCtt                                                                                        >  2:1223085/1‑139 (MQ=255)                                gCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                     <  2:720455/139‑1 (MQ=255)                                   gAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  <  2:823418/139‑1 (MQ=255)                                    aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              <  2:323451/110‑1 (MQ=255)                                    aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACAtt                                                                                                              >  1:323451/1‑110 (MQ=255)                                         cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCg                                                                                                                    <  1:239445/99‑1 (MQ=255)                                         cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCg                                                                                                                    >  2:239445/1‑99 (MQ=255)                                         cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                       >  2:622862/1‑128 (MQ=255)                                         cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                       <  1:622862/128‑1 (MQ=255)                                                     aTTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGtt                                                                >  1:963558/1‑139 (MQ=255)                                                      ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         >  2:10201/1‑97 (MQ=255)                                                      ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         <  1:10201/97‑1 (MQ=255)                                                           gTTAAGGGTTCACGTAGTGTCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                          <  1:823673/139‑1 (MQ=255)                                                           gTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                          <  1:1223085/139‑1 (MQ=255)                                                                  gTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                   <  2:799000/139‑1 (MQ=255)                                                                     cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         <  2:1393095/82‑1 (MQ=255)                                                                     cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTc                                                                                                         >  1:1393095/1‑82 (MQ=255)                                                                            tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCa                                                                                                        <  2:1404684/76‑1 (MQ=255)                                                                            tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCa                                                                                                        >  1:1404684/1‑76 (MQ=255)                                                                              ccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                   >  1:486717/1‑127 (MQ=255)                                                                              ccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                   <  2:486717/127‑1 (MQ=255)                                                                                 ccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                    >  2:1053694/1‑139 (MQ=255)                                                                                     ggAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                          >  2:520912/1‑113 (MQ=255)                                                                                     ggAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                          <  1:520912/113‑1 (MQ=255)                                                                                      gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                           <  1:777046/111‑1 (MQ=255)                                                                                      gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                           >  2:777046/1‑111 (MQ=255)                                                                                      gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGtt                               >  2:1348122/1‑139 (MQ=255)                                                                                            gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                    <  1:773693/128‑1 (MQ=255)                                                                                            gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                    >  2:773693/1‑128 (MQ=255)                                                                                                taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                       >  1:303843/1‑73 (MQ=255)                                                                                                taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                       <  2:303843/73‑1 (MQ=255)                                                                                                taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      <  1:45825/74‑1 (MQ=255)                                                                                                taCGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      >  2:45825/1‑74 (MQ=255)                                                                                                   gcgcTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTgg                  >  2:728020/1‑139 (MQ=255)                                                                                                    cgcTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGa                 <  1:728020/139‑1 (MQ=255)                                                                                                    cgcTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGa                 <  1:1348122/139‑1 (MQ=255)                                                                                                      cTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATg               >  2:1075179/1‑139 (MQ=255)                                                                                                       tttACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATgg              <  1:1075179/139‑1 (MQ=255)                                                                                                         tACATGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            <  1:993263/139‑1 (MQ=255)                                                                                                          aCAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAAtattat                                                                                                <  2:1300463/54‑1 (MQ=255)                                                                                                          aCAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAAtattat                                                                                                >  1:1300463/1‑54 (MQ=255)                                                                                                                 aaTGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            >  2:589088/1‑131 (MQ=255)                                                                                                                 aaTGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc            <  1:589088/131‑1 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  <  2:347737/59‑1 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                  >  1:347737/1‑59 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                      <  2:323594/103‑1 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                      >  1:323594/1‑103 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGtt                               >  2:1166476/1‑110 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGtt                               <  1:1166476/110‑1 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  2:620743/1‑139 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  2:1102019/1‑139 (MQ=255)                                                                                                                   tGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGa  >  2:883789/1‑139 (MQ=255)                                                                                                                         aTGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      <  2:240578/49‑1 (MQ=255)                                                                                                                         aTGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                      >  1:240578/1‑49 (MQ=255)                                                                                                                               |                                                                                                                               TGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGA  >  NC_000913/95882‑96134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑