Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,068,254 G→A *58* (TAG→TAA ymdF → KGG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,068,2540GA100.0% 226.8 / NA 70*58* (TAG→TAAymdFKGG family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/36);  total (34/36)

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAG  >  NC_000913/1068121‑1068385
                                                                                                                                     |                                                                                                                                   
aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                <  1:216840/139‑1 (MQ=255)
aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                <  2:198332/139‑1 (MQ=255)
  cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                <  2:1239514/137‑1 (MQ=255)
  cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                >  1:1239514/1‑137 (MQ=255)
           gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAAt                                                                                                                           <  1:618250/133‑1 (MQ=255)
           gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAAt                                                                                                                           >  2:618250/1‑133 (MQ=255)
           gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGa                                                                                                                     <  2:1353444/139‑1 (MQ=255)
                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTg                                                                                                       >  2:1256561/1‑139 (MQ=255)
                         tAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTgc                                                                                                      >  1:1205674/1‑138 (MQ=255)
                                         cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGc                                                                                                                               <  2:794615/99‑1 (MQ=255)
                                         cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCaaaaa                                                                                     >  1:1113325/1‑139 (MQ=255)
                                         cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGAGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGc                                                                                                                               >  1:794615/1‑99 (MQ=255)
                                            agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                  >  2:428539/1‑139 (MQ=255)
                                            agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGcc                                                                                  >  2:1181183/1‑139 (MQ=255)
                                              cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCAt                                                                                >  1:1097901/1‑139 (MQ=255)
                                                ggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCa                                                                              >  2:469690/1‑139 (MQ=255)
                                                 gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAGGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAc                                                                             >  1:924407/1‑139 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                <  1:835650/88‑1 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                          <  2:848635/109‑1 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                          >  1:848635/1‑109 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGc                                                                                               >  2:188007/1‑119 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGc                                                                                               <  1:188007/119‑1 (MQ=255)
                                                   gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGAGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                >  2:835650/1‑88 (MQ=255)
                                                           aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAc                                                                   >  1:1410786/1‑139 (MQ=255)
                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAATGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  2:924407/139‑1 (MQ=255)
                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  1:428539/139‑1 (MQ=255)
                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  2:1113325/139‑1 (MQ=255)
                                                              aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  1:1181183/139‑1 (MQ=255)
                                                                tGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                >  1:368152/1‑137 (MQ=255)
                                                                tGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  2:368152/137‑1 (MQ=255)
                                                                 gACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                             <  2:1097901/139‑1 (MQ=255)
                                                                  aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtgg                                                            >  2:698613/1‑139 (MQ=255)
                                                                  aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                >  2:709966/1‑135 (MQ=255)
                                                                  aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  1:709966/135‑1 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                >  2:422801/1‑129 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  1:422801/129‑1 (MQ=255)
                                                                           gcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                   >  1:1072824/1‑139 (MQ=255)
                                                                            cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtga                                                  >  2:236832/1‑139 (MQ=255)
                                                                              cATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                              <  2:309809/125‑1 (MQ=255)
                                                                              cATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                              >  1:309809/1‑125 (MQ=255)
                                                                                       cAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTa                                       <  2:1410786/139‑1 (MQ=255)
                                                                                         ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                     >  1:1392818/1‑139 (MQ=255)
                                                                                            aaaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                  <  1:265768/107‑1 (MQ=255)
                                                                                            aaaaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                  >  2:265768/1‑107 (MQ=255)
                                                                                              aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                          >  1:1203054/1‑97 (MQ=255)
                                                                                              aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                          <  2:1203054/97‑1 (MQ=255)
                                                                                              aaaaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGc                                <  2:1072824/139‑1 (MQ=255)
                                                                                                   gtggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                <  2:1312375/40‑1 (MQ=255)
                                                                                                   gtggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                >  1:1312375/1‑40 (MQ=255)
                                                                                                      gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAc                                                                             >  2:545670/1‑86 (MQ=255)
                                                                                                      gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCAc                                                                             <  1:545670/86‑1 (MQ=255)
                                                                                                      gtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                        <  2:775250/139‑1 (MQ=255)
                                                                                                          gagCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcgcaat                    <  2:1392818/139‑4 (MQ=255)
                                                                                                           agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                   <  1:698613/139‑1 (MQ=255)
                                                                                                              aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCt                                                                                              <  1:730416/61‑1 (MQ=255)
                                                                                                              aGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCt                                                                                              >  2:730416/1‑61 (MQ=255)
                                                                                                               gTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCACCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa               <  1:469690/139‑1 (MQ=255)
                                                                                                                tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                  <  1:322924/87‑1 (MQ=255)
                                                                                                                tCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                  >  2:322924/1‑87 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                          >  2:258028/1‑43 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                          <  1:258028/43‑1 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                <  1:1291574/84‑1 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                >  2:1291574/1‑84 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                              <  1:174079/102‑1 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                              >  2:174079/1‑102 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTg                                 <  2:177912/115‑1 (MQ=255)
                                                                                                                     gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTg                                 >  1:177912/1‑115 (MQ=255)
                                                                                                                            cGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCag  <  1:1256561/139‑1 (MQ=255)
                                                                                                                              aCAACTAACCGGACTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                     >  2:66614/1‑102 (MQ=255)
                                                                                                                              aCAACTAACCGGACTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                     <  1:66614/102‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAG  >  NC_000913/1068121‑1068385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: