Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,511	G→A	intergenic (‑24/+50)	dinI ← / ← pyrC	DNA damage‑inducible protein I/dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,511	0	G	A	100.0%	74.6 / NA	24	intergenic (‑24/+50)	dinI/pyrC	DNA damage‑inducible protein I/dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGA  >  NC_000913/1121379‑1121631                                                                                                                                     |                                                                                                                         cATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGttatt                                                                                                                    >  1:612381/1‑139 (MQ=255)           aaaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTa                                                                                                          >  1:618397/1‑139 (MQ=255)           aaaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCCGTTGAATATACTAGTTATTTATACAGGTa                                                                                                          >  1:565133/1‑139 (MQ=255)                cATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                      <  1:1447287/138‑1 (MQ=255)                cATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                      >  2:1447287/1‑138 (MQ=255)                cATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCCGTTGAATATACTAGTTATTTATACAGGTAAAATa                                                                                                     <  2:565133/139‑1 (MQ=255)                       aaTACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAAt                                                                                              <  2:612381/139‑1 (MQ=255)                            ggcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAAt                                                                                              <  1:1094457/134‑1 (MQ=255)                            ggcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAAt                                                                                              >  2:1469154/1‑134 (MQ=255)                            ggcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAAt                                                                                              >  2:1094457/1‑134 (MQ=255)                            ggcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAAt                                                                                              <  1:1469154/134‑1 (MQ=255)                                 gAAAGTTCGCCAGCCAGGGGGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGa                                                                                    >  2:1096047/1‑139 (MQ=255)                                 gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGa                                                                                    >  1:9411/1‑139 (MQ=255)                                                         aTAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACg                                                            <  2:1172842/139‑1 (MQ=255)                                                            gCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAc                                                         <  2:618397/139‑1 (MQ=255)                                                             ccccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAcc                                                        >  2:392794/1‑139 (MQ=255)                                                                      gCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                      >  1:260899/1‑84 (MQ=255)                                                                      gCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAAt                                                                                                      <  2:260899/84‑1 (MQ=255)                                                                                  ttttCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGa                                   <  2:9411/139‑1 (MQ=255)                                                                                                     aaTTCGCATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtc                >  1:1139284/1‑139 (MQ=255)                                                                                                           cATAATAGCCCCCTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagt          <  1:392794/139‑1 (MQ=255)                                                                                                                   cccccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTccc                                         >  2:198220/1‑100 (MQ=255)                                                                                                                   cccccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTccc                                         <  1:198220/100‑1 (MQ=255)                                                                                                                   cccccTGTTGAATATACTAGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGa  >  1:449113/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                         CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGA  >  NC_000913/1121379‑1121631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑