Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,177,670 C→T G117G (GGC→GGT lolE → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,177,6700CT100.0% 94.7 / NA 31G117G (GGC→GGTlolElipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

GCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGA  >  NC_000913/1177553‑1177800
                                                                                                                     |                                                                                                                                  
gCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAg                                                                                                               <  2:1460317/139‑1 (MQ=255)
                     gCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg                                                                                          >  1:867374/1‑139 (MQ=255)
                                              gccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                              <  1:1237180/110‑1 (MQ=255)
                                              gccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                              >  2:1237180/1‑110 (MQ=255)
                                                cgcgCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt                                                               <  2:867374/139‑1 (MQ=255)
                                                       tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                                   >  1:1027676/1‑128 (MQ=255)
                                                       tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                                   <  2:1027676/128‑1 (MQ=255)
                                                       tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                                   <  2:481072/128‑1 (MQ=255)
                                                       tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                                   >  1:481072/1‑128 (MQ=255)
                                                             aaTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCg                                                  <  1:1205693/139‑1 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGc                                                                                                                          <  2:857219/63‑1 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGc                                                                                                                          >  1:857219/1‑63 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCg                                                                             <  1:225256/108‑1 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCg                                                                             >  2:225256/1‑108 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACa                                              >  1:1227060/1‑139 (MQ=255)
                                                                 tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACa                                              >  2:28331/1‑139 (MQ=255)
                                                                  cACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAg                                             <  2:1227060/139‑1 (MQ=255)
                                                                     cGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCaa                                          >  1:235437/1‑139 (MQ=255)
                                                                           ggtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatc                                    <  1:1102188/139‑1 (MQ=255)
                                                                                  aGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                              >  1:221176/1‑74 (MQ=255)
                                                                                  aGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                              <  2:221176/74‑1 (MQ=255)
                                                                                  aGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCa                             >  1:1400009/1‑139 (MQ=255)
                                                                                      gcgcGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGc                                                                                                                          <  1:105202/42‑1 (MQ=255)
                                                                                      gcgcGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGc                                                                                                                          >  2:105202/1‑42 (MQ=255)
                                                                                      gcgcGAATCTTCGCGCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAgg                         >  2:560468/1‑139 (MQ=255)
                                                                                                 cgcgCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATg              <  1:560468/139‑1 (MQ=255)
                                                                                                 cgcgCAATCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATg              >  1:1347947/1‑139 (MQ=255)
                                                                                                      aaTCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCaaa                           <  1:991848/121‑1 (MQ=255)
                                                                                                      aaTCCAGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCaaa                           >  2:991848/1‑121 (MQ=255)
                                                                                                           aGGTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCCGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGt    <  2:924264/139‑1 (MQ=255)
                                                                                                             gTGAAGGGTGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGa  >  1:152691/1‑139 (MQ=255)
                                                                                                                     |                                                                                                                                  
GCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGCGCTGAAAGTGA  >  NC_000913/1177553‑1177800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: