Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,238	A→G	V121A (GTG→GCG)	zapD ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,238	0	A	G	100.0%	209.4 / NA	58	V121A (GTG→GCG)	zapD	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/29);  total (29/29)

GGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAA  >  NC_000913/112104‑112367                                                                                                                                       |                                                                                                                                  gggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCag                                                                                                                               >  2:134851/1‑139 (MQ=255)  ggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCaga                                                                                                                              >  2:676530/1‑139 (MQ=255)            ttCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAAc                                                                                                                    >  1:1273413/1‑139 (MQ=255)            ttCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAAc                                                                                                                    <  1:246724/139‑1 (MQ=255)                            aGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaa                                                                                                    <  2:1273413/139‑1 (MQ=255)                              gTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                                                                  <  1:676530/139‑1 (MQ=255)                                ttCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGc                                                                                                <  1:134851/139‑1 (MQ=255)                                   tACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                                                                  <  2:293534/134‑1 (MQ=255)                                   tACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                                                                  >  1:293534/1‑134 (MQ=255)                                    aCCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGa                                                                                            >  2:1396996/1‑139 (MQ=255)                                             tCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcg                                                                                   <  2:1444188/139‑1 (MQ=255)                                               gcgcTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaa                                                                                                    <  1:1226013/120‑1 (MQ=255)                                               gcgcTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaa                                                                                                    >  2:1226013/1‑120 (MQ=255)                                                         tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                                                                       <  1:1396996/139‑1 (MQ=255)                                                                gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              <  1:84643/93‑1 (MQ=255)                                                                gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              >  2:84643/1‑93 (MQ=255)                                                                gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcg                                                                                 <  1:917246/122‑1 (MQ=255)                                                                gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcg                                                                                 >  2:917246/1‑122 (MQ=255)                                                                  tGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGa                                                                               >  2:29065/1‑122 (MQ=255)                                                                  tGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGa                                                                               <  1:29065/122‑1 (MQ=255)                                                                          aaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt                                                      <  1:537131/139‑1 (MQ=255)                                                                            tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa                                                    >  2:458520/1‑139 (MQ=255)                                                                                     tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt                                           >  2:772944/1‑139 (MQ=255)                                                                                        gTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcg                                                                                      <  2:1376110/93‑1 (MQ=255)                                                                                        gTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcg                                                                                      >  1:1376110/1‑93 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              >  1:1294565/1‑64 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              <  2:1294565/64‑1 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACg                                                                                                          <  1:625788/68‑1 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACg                                                                                                          >  2:625788/1‑68 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt                                           >  2:1343654/1‑131 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt                                           <  1:1343654/131‑1 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCtt                                   >  2:609624/1‑139 (MQ=255)                                                                                             tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCtt                                   >  1:1197958/1‑139 (MQ=255)                                                                                               aaaGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                                                                     <  1:1057831/55‑1 (MQ=255)                                                                                               aaaGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                                                                     >  2:1057831/1‑55 (MQ=255)                                                                                                 aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              >  2:430170/1‑60 (MQ=255)                                                                                                 aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                                                              <  1:430170/60‑1 (MQ=255)                                                                                                  gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                              <  1:609624/139‑1 (MQ=255)                                                                                                  gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                              <  1:458520/139‑1 (MQ=255)                                                                                                   cTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                             <  2:994950/139‑1 (MQ=255)                                                                                                      cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAAtt                                                                           <  2:224883/90‑1 (MQ=255)                                                                                                      cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAAtt                                                                           >  1:224883/1‑90 (MQ=255)                                                                                                      cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt                                                      <  2:1267066/111‑1 (MQ=255)                                                                                                      cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt                                                      >  1:1267066/1‑111 (MQ=255)                                                                                                       aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcg                                                                                 <  2:620948/83‑1 (MQ=255)                                                                                                       aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcg                                                                                 >  1:620948/1‑83 (MQ=255)                                                                                                              gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                                                        >  2:972229/1‑69 (MQ=255)                                                                                                              gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                                                        <  1:972229/69‑1 (MQ=255)                                                                                                                      tGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAgg          <  1:772944/139‑1 (MQ=255)                                                                                                                        cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACg               >  1:851777/1‑132 (MQ=255)                                                                                                                        cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACg               <  2:851777/132‑1 (MQ=255)                                                                                                                        cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCa        >  2:7608/1‑139 (MQ=255)                                                                                                                             tCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGACCGATACGCGGAGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGATTCAATACGGCTCTGGTCCACGCCAGGAAGGCCa   >  2:1192451/1‑139 (MQ=255)                                                                                                                              cGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCaa  >  1:1195483/1‑139 (MQ=255)                                                                                                                                cTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCAc       >  1:154713/1‑132 (MQ=255)                                                                                                                                cTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCAc       <  2:154713/132‑1 (MQ=255)                                                                                                                                   aCGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                        <  1:1267010/96‑1 (MQ=255)                                                                                                                                   aCGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                        >  2:1267010/1‑96 (MQ=255)                                                                                                                                       |                                                                                                                                  GGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAA  >  NC_000913/112104‑112367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑