Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,271,455 G→A T132I (ACC→ATC)  chaA ← calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,271,4550GA100.0% 172.4 / NA 53T132I (ACC→ATC) chaAcalcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/28);  total (25/28)

CCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGC  >  NC_000913/1271330‑1271563
                                                                                                                             |                                                                                                            
ccAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACg                                                                                                 >  1:359173/1‑139 (MQ=255)
                 gTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                <  2:1199008/139‑1 (MQ=255)
                  ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        >  2:1424850/1‑130 (MQ=255)
                  ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        <  1:1424850/130‑1 (MQ=255)
                  ttGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGa                                                                               >  2:952906/1‑139 (MQ=255)
                            cgccgcAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        >  2:1469586/1‑120 (MQ=255)
                            cgccgcAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        <  1:1469586/120‑1 (MQ=255)
                              ccgcAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAg                                                                   <  2:910845/139‑1 (MQ=255)
                              ccgcAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAg                                                                   <  2:446312/139‑1 (MQ=255)
                               cgcAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGc                                                                  >  2:113344/1‑139 (MQ=255)
                                   ggcagAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCAc                                                              <  1:113344/139‑1 (MQ=255)
                                       gagCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                          <  1:952906/139‑1 (MQ=255)
                                           cATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACa                                                      <  2:359173/139‑1 (MQ=255)
                                              tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGccc                                                                >  2:463627/1‑126 (MQ=255)
                                              tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGccc                                                                <  1:463627/126‑1 (MQ=255)
                                              tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCa                                                               >  1:1504873/1‑127 (MQ=255)
                                              tGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCa                                                               <  2:1504873/127‑1 (MQ=255)
                                                gAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                 <  1:946695/139‑1 (MQ=255)
                                                  aaTACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGTCAAACTTCCGACCCCCCACCAATAATGAAAACCCAACCAGCCCACCGGTAACAATCATaa                                               <  2:30901/139‑1 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                          <  1:603247/78‑1 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                          >  2:603247/1‑78 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                      >  2:915988/1‑114 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                      <  1:915988/114‑1 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                          >  2:1284232/1‑126 (MQ=255)
                                                    tACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                          <  1:1284232/126‑1 (MQ=255)
                                                          tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                       >  2:1436799/1‑139 (MQ=255)
                                                                 aTCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                >  2:250653/1‑91 (MQ=255)
                                                                 aTCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                <  1:250653/91‑1 (MQ=255)
                                                                        gggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                 >  1:707842/1‑115 (MQ=255)
                                                                        gggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                 <  2:707842/115‑1 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                          >  2:1191843/1‑57 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                          <  1:1191843/57‑1 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCa                                                                                                         >  2:191188/1‑58 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCa                                                                                                         <  1:191188/58‑1 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                          >  1:957038/1‑73 (MQ=255)
                                                                         ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                          <  2:957038/73‑1 (MQ=255)
                                                                                aGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTAc                                                                                                  >  2:1272549/1‑58 (MQ=255)
                                                                                aGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTAc                                                                                                  <  1:1272549/58‑1 (MQ=255)
                                                                                  cgcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                <  2:16866/74‑1 (MQ=255)
                                                                                  cgcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                >  1:16866/1‑74 (MQ=255)
                                                                                  cgcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAg                                                                           <  1:693658/79‑1 (MQ=255)
                                                                                  cgcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAg                                                                           >  2:693658/1‑79 (MQ=255)
                                                                                               tGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGc  >  1:782616/1‑139 (MQ=255)
                                                                                                      tACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAg                                                                   >  2:1339871/1‑67 (MQ=255)
                                                                                                      tACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAg                                                                   <  1:1339871/67‑1 (MQ=255)
                                                                                                       aCCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCa                                                                        <  2:84491/61‑1 (MQ=255)
                                                                                                       aCCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCa                                                                        >  1:84491/1‑61 (MQ=255)
                                                                                                           aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        >  2:1152571/1‑41 (MQ=255)
                                                                                                           aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                        <  1:1152571/41‑1 (MQ=255)
                                                                                                           aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATa                                     >  2:195811/1‑92 (MQ=255)
                                                                                                           aaCAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATa                                     <  1:195811/92‑1 (MQ=255)
                                                                                                                aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                          >  2:1415846/1‑66 (MQ=255)
                                                                                                                aTTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                          <  1:1415846/66‑1 (MQ=255)
                                                                                                                             |                                                                                                            
CCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGC  >  NC_000913/1271330‑1271563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: