Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,309,016 C→T *418* (TAG→TAA)  kch ← voltage‑gated potassium channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,309,0160CT100.0% 196.8 / NA 61*418* (TAG→TAA) kchvoltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/30);  total (31/30)

TCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCAT  >  NC_000913/1308891‑1309137
                                                                                                                             |                                                                                                                         
tCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                              <  1:289085/139‑1 (MQ=255)
                 tGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                             >  2:1260705/1‑139 (MQ=255)
                  gCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCtt                                                                                            <  1:1260705/139‑1 (MQ=255)
                           acaaacaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                <  2:1234615/110‑1 (MQ=255)
                           acaaacaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                >  1:1234615/1‑110 (MQ=255)
                               acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTAGTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCt                                                                                                         >  2:1079598/1‑113 (MQ=255)
                               acaGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCt                                                                                                         <  1:1079598/113‑1 (MQ=255)
                                  gCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                      <  1:1429518/129‑1 (MQ=255)
                                  gCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGttt                                                                                      >  2:1429518/1‑129 (MQ=255)
                                             tATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                >  1:195807/1‑92 (MQ=255)
                                             tATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                <  2:195807/92‑1 (MQ=255)
                                                                   cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                           <  2:1356969/139‑1 (MQ=255)
                                                                   cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                           >  1:1356969/1‑139 (MQ=255)
                                                                        tGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                >  2:630181/1‑65 (MQ=255)
                                                                        tGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                <  1:630181/65‑1 (MQ=255)
                                                                           aCTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTg                                   >  2:1355602/1‑139 (MQ=255)
                                                                            cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                >  1:1493386/1‑61 (MQ=255)
                                                                            cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                <  2:1493386/61‑1 (MQ=255)
                                                                            cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                              >  2:695582/1‑63 (MQ=255)
                                                                            cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCg                                                                                                              <  1:695582/63‑1 (MQ=255)
                                                                            cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                                  >  2:651699/1‑139 (MQ=255)
                                                                             tGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaa                                                                >  1:1158264/1‑108 (MQ=255)
                                                                             tGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGaa                                                                <  2:1158264/108‑1 (MQ=255)
                                                                               ggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAAc                               <  1:651699/139‑1 (MQ=255)
                                                                                    cATTGCGGTGCCCAATACGTTTACTCTTCCGTCTTTAAACTTTAtttt                                                                                                                     <  2:1127111/48‑1 (MQ=255)
                                                                                    cATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACGTTAtttt                                                                                                                     >  1:1127111/1‑48 (MQ=255)
                                                                                      ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                   <  2:669501/112‑1 (MQ=255)
                                                                                      ttGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                                   >  1:669501/1‑112 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                >  1:779048/1‑43 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTgcgc                                                                                                                <  2:779048/43‑1 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                             <  1:469557/62‑1 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                             >  2:469557/1‑62 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                    <  1:704696/87‑1 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                    >  2:704696/1‑87 (MQ=255)
                                                                                              cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt                >  1:891419/1‑139 (MQ=255)
                                                                                               ccAATAAGTATACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCTGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta               <  2:891419/139‑1 (MQ=255)
                                                                                                   tAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGt                                                                          >  2:1191613/1‑76 (MQ=255)
                                                                                                   tAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGt                                                                          <  1:1191613/76‑1 (MQ=255)
                                                                                                        ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAAt                                                             <  1:1194880/84‑1 (MQ=255)
                                                                                                        ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAAt                                                             >  2:1194880/1‑84 (MQ=255)
                                                                                                           ctctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCt                                                                   >  2:134325/1‑75 (MQ=255)
                                                                                                           ctctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCt                                                                   <  1:134325/75‑1 (MQ=255)
                                                                                                            tctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAAt             <  1:155285/128‑1 (MQ=255)
                                                                                                            tctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAAt             >  2:155285/1‑128 (MQ=255)
                                                                                                            tctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCAt  >  2:384393/1‑139 (MQ=255)
                                                                                                             ctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                             <  1:566659/47‑1 (MQ=255)
                                                                                                             ctTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                             >  2:566659/1‑47 (MQ=255)
                                                                                                                ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTa                                                                        >  1:654350/1‑65 (MQ=255)
                                                                                                                ccGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTa                                                                        <  2:654350/65‑1 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCt                                                                   <  2:1028493/69‑1 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCt                                                                   >  1:1028493/1‑69 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                           >  1:54015/1‑93 (MQ=255)
                                                                                                                 cGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                           <  2:54015/93‑1 (MQ=255)
                                                                                                                   tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                                  >  1:67497/1‑52 (MQ=255)
                                                                                                                   tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                                  <  2:67497/52‑1 (MQ=255)
                                                                                                                   tCTTTAAACTTTATTTTTGCGCCGATT‑TTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAg                                                <  2:1146188/85‑1 (MQ=255)
                                                                                                                   tCTTTAAACTTTATTTTTGCGCCGATT‑TTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAg                                                >  1:1146188/1‑85 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATc                                                                      >  2:1483644/1‑61 (MQ=255)
                                                                                                                      ttAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATc                                                                      <  1:1483644/61‑1 (MQ=255)
                                                                                                                        aaaCTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAg                            <  1:1259847/101‑1 (MQ=255)
                                                                                                                        aaaCTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAg                            >  2:1259847/1‑101 (MQ=255)
                                                                                                                             |                                                                                                                         
TCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCACCAT  >  NC_000913/1308891‑1309137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑