Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,389,499 C→T C190C (TGC→TGT)  ycjG → L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,389,4990CT100.0% 191.5 / NA 60C190C (TGC→TGT) ycjGL‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/30);  total (30/30)

GAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NC_000913/1389364‑1389633
                                                                                                                                       |                                                                                                                                      
gAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAg                                                                                                                                     <  1:704856/139‑1 (MQ=255)
  aaTTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCt                                                                                                                                   >  1:194302/1‑139 (MQ=255)
                       aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGTTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  2:912052/139‑1 (MQ=255)
                             aTCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCATAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCt                                                                                                        <  2:1045745/139‑1 (MQ=255)
                                  aTCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              >  2:116946/1‑128 (MQ=255)
                                  aTCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  1:116946/128‑1 (MQ=255)
                                     aGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGc                                                                                                         <  2:367305/130‑1 (MQ=255)
                                     aGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGc                                                                                                         >  1:367305/1‑130 (MQ=255)
                                         aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCt                                                                                            >  2:149059/1‑139 (MQ=255)
                                         aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGACGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCt                                                                                            >  2:814711/1‑139 (MQ=255)
                                          gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTTGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              >  1:582189/1‑120 (MQ=255)
                                          gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  2:582189/120‑1 (MQ=255)
                                                        ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGa                                                                             >  1:229051/1‑139 (MQ=255)
                                                                  tgtgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTg                                                                   <  1:149059/139‑1 (MQ=255)
                                                                  tgtgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAAGACGATGCGGCGCTg                                                                   >  2:41135/1‑139 (MQ=255)
                                                                    tgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGTGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     <  2:613359/119‑1 (MQ=255)
                                                                    tgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGTGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     >  1:613359/1‑119 (MQ=255)
                                                                    tgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 <  1:1234247/139‑1 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTgg                                                                                                                              >  1:1131153/1‑71 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTgg                                                                                                                              <  2:1131153/71‑1 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGa                                                                                                     <  1:919762/96‑1 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGa                                                                                                     >  2:919762/1‑96 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:1296545/1‑139 (MQ=255)
                                                                           tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                          >  2:981398/1‑139 (MQ=255)
                                                                             cGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTAt                                                        <  1:814711/139‑1 (MQ=255)
                                                                               aCGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTc                                                      <  2:194302/139‑1 (MQ=255)
                                                                                cGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCa                                                                                   >  1:585909/1‑109 (MQ=255)
                                                                                cGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCa                                                                                   <  2:585909/109‑1 (MQ=255)
                                                                                  cTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATc                                                   <  1:1296545/139‑1 (MQ=255)
                                                                                           gATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGa                                          >  2:14937/1‑139 (MQ=255)
                                                                                             tGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTgg                                                                                                                              <  2:344237/53‑1 (MQ=255)
                                                                                             tGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtt                                                            <  1:108305/119‑1 (MQ=255)
                                                                                             tGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtt                                                            >  2:108305/1‑119 (MQ=255)
                                                                                             tGCAAATGAATCCTGGCGTGAAGAAGGGTTGGCGGCGCGTTGTCAGCTATTgg                                                                                                                              >  1:344237/1‑53 (MQ=39)
                                                                                               cAAATGAATCCTGGCGTGCATAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  1:700503/1‑120 (MQ=255)
                                                                                               cAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         <  2:700503/120‑1 (MQ=255)
                                                                                               cAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTtg                                      >  2:1060646/1‑139 (MQ=255)
                                                                                                       tCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgatg                              >  1:361948/1‑139 (MQ=255)
                                                                                                           ggCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCAt                                                    <  1:1062902/113‑1 (MQ=255)
                                                                                                           ggCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCAt                                                    >  2:1062902/1‑113 (MQ=255)
                                                                                                            gCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGt                         >  2:1099863/1‑139 (MQ=255)
                                                                                                               tGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGt                      >  2:381013/1‑139 (MQ=255)
                                                                                                                 cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     >  2:1415996/1‑74 (MQ=255)
                                                                                                                 cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     <  1:1415996/74‑1 (MQ=255)
                                                                                                                 cAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTGGCGATGCTTGATCAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCa                    <  2:229051/139‑1 (MQ=255)
                                                                                                                    aaGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATAc                 <  1:981398/139‑1 (MQ=255)
                                                                                                                          tGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAAGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAg           <  1:41135/139‑1 (MQ=255)
                                                                                                                           ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          >  1:1021925/1‑59 (MQ=255)
                                                                                                                           ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:1021925/59‑1 (MQ=255)
                                                                                                                           ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 >  2:868380/1‑84 (MQ=255)
                                                                                                                           ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 <  1:868380/84‑1 (MQ=255)
                                                                                                                                 gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 >  1:894670/1‑78 (MQ=255)
                                                                                                                                 gcgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGga                                                                 <  2:894670/78‑1 (MQ=255)
                                                                                                                                  cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaaca                                                                                                  <  2:1154199/44‑1 (MQ=255)
                                                                                                                                  cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaaca                                                                                                  >  1:1154199/1‑44 (MQ=255)
                                                                                                                                  cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           >  2:1170360/1‑67 (MQ=255)
                                                                                                                                  cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           <  1:1170360/67‑1 (MQ=255)
                                                                                                                                   gTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGa                                                                                <  2:894585/61‑1 (MQ=255)
                                                                                                                                   gTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGa                                                                                >  1:894585/1‑61 (MQ=255)
                                                                                                                                   gTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGaa  <  1:1060646/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NC_000913/1389364‑1389633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑