Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,386	G→A	intergenic (‑157/‑285)	kilR ← / → sieB	killing protein, Rac prophage, FtsZ inhibitor protein/phage superinfection exclusion protein, Rac prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,386	0	G	A	100.0%	171.6 / NA	54	intergenic (‑157/‑285)	kilR/sieB	killing protein, Rac prophage, FtsZ inhibitor protein/phage superinfection exclusion protein, Rac prophage
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/26);  total (28/26)

TTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418260‑1418507                                                                                                                               |                                                                                                                          ttgATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAAtt                                                                                                               >  1:722960/1‑139 (MQ=255)           gtCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTaaaa                                                                                                     >  2:1028378/1‑139 (MQ=255)            tCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTaaaaa                                                                                                    >  2:24605/1‑139 (MQ=255)               ggTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAggg                                                                                                 <  2:922918/139‑1 (MQ=255)                 tGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCg                                                                                               <  2:1432176/139‑1 (MQ=255)                      ccAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGAc                                                                                            >  2:1478744/1‑137 (MQ=255)                      ccAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGAc                                                                                            <  1:1478744/137‑1 (MQ=255)                      ccAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACAt                                                                                          <  1:843368/139‑1 (MQ=255)                          gTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGc                                                                                      >  1:1036983/1‑139 (MQ=255)                                   aCCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAggg                                                                                                 >  1:318592/1‑119 (MQ=255)                                   aCCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAggg                                                                                                 <  2:318592/119‑1 (MQ=255)                                        ttAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGaaa                                                                        >  1:1454069/1‑139 (MQ=255)                                                tACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCa                                                                             <  2:900908/126‑1 (MQ=255)                                                tACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCa                                                                             >  1:900908/1‑126 (MQ=255)                                                 aCAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGAt                                                               <  2:1036983/139‑1 (MQ=255)                                                  cAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGAt                                                               >  1:1179665/1‑138 (MQ=255)                                                  cAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGAt                                                               <  2:1179665/138‑1 (MQ=255)                                                      cGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATgccgc                                                          <  2:591202/139‑1 (MQ=255)                                                             cccacccaAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACt                                                   <  1:1028378/139‑1 (MQ=255)                                                                 cccaAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATc                                                                                                                       >  1:801417/1‑67 (MQ=255)                                                                 cccaAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATc                                                                                                                       <  2:801417/67‑1 (MQ=255)                                                                      aCCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATgccgc                                                          >  2:840047/1‑123 (MQ=255)                                                                      aCCAATAACGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATgccgc                                                          <  1:840047/123‑1 (MQ=255)                                                                            aaGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTc                                    >  2:754044/1‑139 (MQ=255)                                                                                  tAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCg                                   >  1:853126/1‑134 (MQ=255)                                                                                  tAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCg                                   <  2:853126/134‑1 (MQ=255)                                                                                    aCATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTa                            >  2:574977/1‑139 (MQ=255)                                                                                     cATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACAt                                                                                          <  2:754951/76‑1 (MQ=255)                                                                                     cATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACAt                                                                                          >  1:754951/1‑76 (MQ=255)                                                                                        gACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAAc                        <  2:1250433/139‑1 (MQ=255)                                                                                         aCTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAAcc                       >  1:795550/1‑139 (MQ=255)                                                                                         aCTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAAcc                       >  1:1436847/1‑139 (MQ=255)                                                                                          cTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTg                                                                 <  2:21471/96‑1 (MQ=255)                                                                                          cTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTg                                                                 >  1:21471/1‑96 (MQ=255)                                                                                              tAACTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGGCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAgg                  <  2:1454069/139‑1 (MQ=255)                                                                                                aCTGTGCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGgcg                <  1:24605/139‑1 (MQ=255)                                                                                                   gtgCCACGTGCGCTTAGCCGCATTCACCACATCACAAAAt                                                                                                                <  2:873651/40‑1 (MQ=17)                                                                                                   gtgCCACGTGCGCTTAGCCGCATTCACCACATCACAAAAt                                                                                                                >  1:873651/1‑40 (MQ=17)                                                                                                    tgCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATg                                      >  2:855986/1‑113 (MQ=255)                                                                                                    tgCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATg                                      <  1:855986/113‑1 (MQ=255)                                                                                                    tgCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAGCCGGAGGCGCACt            >  1:78924/1‑139 (MQ=255)                                                                                                     gCCACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTc           >  2:398321/1‑139 (MQ=255)                                                                                                      ccACGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTGAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTcc          >  2:1066939/1‑139 (MQ=255)                                                                                                        aCGTGCGCTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCcca        <  1:754044/139‑1 (MQ=255)                                                                                                            gcgcTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCa                                                        >  2:1271854/1‑87 (MQ=255)                                                                                                            gcgcTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCa                                                        <  1:1271854/87‑1 (MQ=255)                                                                                                              gcTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCattt  <  2:795550/139‑1 (MQ=255)                                                                                                              gcTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCattt  >  1:969038/1‑139 (MQ=255)                                                                                                               cTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTc                                                                              >  1:777512/1‑62 (MQ=255)                                                                                                               cTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTc                                                                              <  2:777512/62‑1 (MQ=255)                                                                                                               cTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaca                                                <  2:139767/92‑1 (MQ=255)                                                                                                               cTTAGCCGCATTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTaca                                                >  1:139767/1‑92 (MQ=255)                                                                                                                        aTTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATg                                      >  2:526108/1‑93 (MQ=255)                                                                                                                        aTTCACCACATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATg                                      <  1:526108/93‑1 (MQ=255)                                                                                                                               |                                                                                                                          TTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTTTAAAAAGGGCGGACATCAGCCGAACTTCAAGAAAAAAACTGATGCCGCCAGGACTACACACAGCAATGTCGTTATTTACAACCGGAGGCGCACTCCCACCATTT  >  NC_000913/1418260‑1418507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑