Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,308	A→G	E151E (GAA→GAG)	opgD →	OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,308	0	A	G	100.0%	191.9 / NA	53	E151E (GAA→GAG)	opgD	OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

CCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATA  >  NC_000913/1497173‑1497420                                                                                                                                        |                                                                                                                 ccttatGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCTTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAgggg                                                                                                               <  2:974455/136‑1 (MQ=255)    tatGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCaa                                                                                                            >  1:1044867/1‑139 (MQ=255)    tatGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCaa                                                                                                            >  1:243646/1‑139 (MQ=255)      tgggaatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAg                                                                                                          >  1:534145/1‑139 (MQ=255)          aatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAt                                                                                                      >  2:1387086/1‑139 (MQ=255)           atgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGAtc                                                                                                     >  2:645849/1‑139 (MQ=255)              ggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCCGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCg                                                                                                  >  1:243548/1‑139 (MQ=255)                    cGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCaaa                                                                                                           <  2:141860/124‑1 (MQ=255)                    cGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCaaa                                                                                                           >  1:141860/1‑124 (MQ=255)                                         tCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACACCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                 <  1:1129828/129‑1 (MQ=255)                                         tCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                 >  2:1129828/1‑129 (MQ=255)                                                     gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTg                                                            >  2:1495936/1‑138 (MQ=255)                                                     gCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTg                                                            <  1:1495936/138‑1 (MQ=255)                                                           caTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGcc                                                     >  1:1104261/1‑139 (MQ=255)                                                            aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                    <  2:1044867/139‑1 (MQ=255)                                                            aTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCg                                                    <  2:243548/139‑1 (MQ=255)                                                              cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg                                                  <  1:813928/139‑1 (MQ=255)                                                              cTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTg                                                  >  1:906178/1‑139 (MQ=255)                                                                 gcgcGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATg                                               >  1:574713/1‑139 (MQ=255)                                                                      tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                         >  1:268475/1‑124 (MQ=255)                                                                      tGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                         <  2:268475/124‑1 (MQ=255)                                                                                   cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                             <  2:513474/139‑1 (MQ=255)                                                                                   cGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                             <  2:1273735/139‑1 (MQ=255)                                                                                     cccGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                              >  1:734803/1‑120 (MQ=255)                                                                                     cccGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATgt                                              <  2:734803/120‑1 (MQ=255)                                                                                                  aaaTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg              <  1:1387086/139‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc            <  2:534145/139‑1 (MQ=255)                                                                                                    aTACAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGcc            >  1:1141388/1‑139 (MQ=255)                                                                                                      aCAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg              >  1:1333001/1‑135 (MQ=255)                                                                                                      aCAACGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCcgcg              <  2:1333001/135‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTgg                                                           <  1:1346611/87‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTgg                                                           >  2:1346611/1‑87 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga       <  1:645849/139‑1 (MQ=255)                                                                                                         aCGATGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtga       <  2:243646/139‑1 (MQ=255)                                                                                                          cGAGGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTGTTTCCGCGCCGTtgat      >  1:1026756/1‑139 (MQ=255)                                                                                                             tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTaaa                                                                        >  1:1020766/1‑70 (MQ=255)                                                                                                             tGCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTaaa                                                                        <  2:1020766/70‑1 (MQ=255)                                                                                                              gCAGGTGTTGATGCAAAACAATTTGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                 <  1:960033/60‑1 (MQ=255)                                                                                                              gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgc                                                                                 >  2:960033/1‑60 (MQ=255)                                                                                                              gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa  <  2:1026756/139‑1 (MQ=255)                                                                                                              gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa  <  1:684397/139‑1 (MQ=255)                                                                                                              gCAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATa  <  2:574713/139‑1 (MQ=255)                                                                                                               cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgcg                                                                                <  1:681853/60‑1 (MQ=255)                                                                                                               cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTcgcg                                                                                >  2:681853/1‑60 (MQ=255)                                                                                                               cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                             >  2:1492008/1‑111 (MQ=255)                                                                                                               cAGGTGTTGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGgcg                             <  1:1492008/111‑1 (MQ=255)                                                                                                                     ttGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcgcg                                                       >  1:640413/1‑79 (MQ=255)                                                                                                                     ttGATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcgcg                                                       <  2:640413/79‑1 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                         >  1:1453104/1‑75 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGgcg                                                         <  2:1453104/75‑1 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtgatga    <  1:1144261/128‑1 (MQ=255)                                                                                                                       gATACAAAACAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTtgatga    >  2:1144261/1‑128 (MQ=255)                                                                                                                             aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGc                                                                      <  1:1325006/56‑1 (MQ=255)                                                                                                                             aaaCAATTAGAGGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGc                                                                      >  2:1325006/1‑56 (MQ=255)                                                                                                                                        |                                                                                                                 CCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATA  >  NC_000913/1497173‑1497420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑