Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	138,386	C→T	S435F (TCT→TTT)	cueO →	multicopper oxidase (laccase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	138,386	0	C	T	100.0%	233.4 / NA	71	S435F (TCT→TTT)	cueO	multicopper oxidase (laccase)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/35);  total (36/35)

GAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAA  >  NC_000913/138270‑138515                                                                                                                     |                                                                                                                                  gAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgctg                                                                                                             >  1:1401669/1‑139 (MQ=255)     cACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATc                                                                                                         >  2:1262113/1‑139 (MQ=255)      aCGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATcc                                                                                                        >  2:1288278/1‑139 (MQ=255)        ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACatg                                                                                                                   <  2:178435/126‑1 (MQ=255)        ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACatg                                                                                                                   >  1:178435/1‑126 (MQ=255)        ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                      >  1:782726/1‑139 (MQ=255)        ggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                                      >  2:1115565/1‑139 (MQ=255)           gggAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTcc                                                                                                   <  2:266516/139‑1 (MQ=255)            ggAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCa                                                                                                  >  1:900210/1‑139 (MQ=255)            ggAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCa                                                                                                  >  1:765104/1‑139 (MQ=255)            ggAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCa                                                                                                  >  2:280083/1‑139 (MQ=255)                         ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  2:900210/139‑1 (MQ=255)                         ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  1:280083/139‑1 (MQ=255)                         ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  1:1262113/139‑1 (MQ=255)                         ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  2:291058/139‑1 (MQ=255)                          caccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                                    <  2:765104/139‑1 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCg                                                                                                                              <  1:1357539/91‑1 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCg                                                                                                                              >  2:1357539/1‑91 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgct                                                                                                              <  2:1484484/107‑1 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgct                                                                                                              >  1:1484484/1‑107 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCa                                                                                   >  1:1411512/1‑134 (MQ=255)                                gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCa                                                                                   <  2:1411512/134‑1 (MQ=255)                                     caaAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATc                                                                         <  2:782726/139‑1 (MQ=255)                                     caaAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATc                                                                         >  2:1235273/1‑139 (MQ=255)                                              cGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGc                                                                                                                         <  2:244067/82‑1 (MQ=255)                                              cGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGc                                                                                                                         >  1:244067/1‑82 (MQ=255)                                                   aGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATgg                                                           <  1:1235273/139‑1 (MQ=255)                                                        tttGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTc                                                                                                    <  2:610445/93‑1 (MQ=255)                                                        tttGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTc                                                                                                    >  1:610445/1‑93 (MQ=255)                                                          tGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTgg                                                                                                                                >  2:1099769/1‑63 (MQ=255)                                                          tGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTgg                                                                                                                                <  1:1099769/63‑1 (MQ=255)                                                          tGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgc                                                                                                               <  2:605509/80‑1 (MQ=255)                                                          tGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgc                                                                                                               >  1:605509/1‑80 (MQ=255)                                                          tGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCg                                                    >  2:537232/1‑139 (MQ=255)                                                               tGAACAATCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATcc                                                                                                        >  2:988547/1‑82 (MQ=255)                                                               tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTggg                                                                                                                          >  2:851558/1‑64 (MQ=255)                                                               tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTggg                                                                                                                          <  1:851558/64‑1 (MQ=255)                                                               tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATcc                                                                                                        <  1:988547/82‑1 (MQ=255)                                                               tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCa                                                 <  2:525824/137‑1 (MQ=255)                                                               tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCa                                                 >  1:525824/1‑137 (MQ=255)                                                                 aaCAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCgg                                             <  1:1115565/139‑1 (MQ=255)                                                                  aCAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGc                                            >  2:1126519/1‑139 (MQ=255)                                                                      gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgc                                                                                                               <  1:698072/68‑1 (MQ=255)                                                                      gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGAtgc                                                                                                               >  2:698072/1‑68 (MQ=255)                                                                      gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCa                                                                                            <  2:719187/87‑1 (MQ=255)                                                                      gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCa                                                                                            >  1:719187/1‑87 (MQ=255)                                                                      gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCAt                                        >  2:787785/1‑139 (MQ=255)                                                                       ccGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  2:349354/93‑1 (MQ=255)                                                                       ccGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     >  1:349354/1‑93 (MQ=255)                                                                          aTGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                                    <  2:1401669/139‑1 (MQ=255)                                                                          aTGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                                    <  1:1126519/139‑1 (MQ=255)                                                                               tGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                               <  1:253829/139‑1 (MQ=255)                                                                                gcggcggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     <  2:1308213/84‑1 (MQ=255)                                                                                gcggcggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                                     >  1:1308213/1‑84 (MQ=255)                                                                                    cggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                                    <  1:1197922/81‑1 (MQ=255)                                                                                    cggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                                    >  2:1197922/1‑81 (MQ=255)                                                                                    cggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTAt                                                                          <  1:1147484/91‑1 (MQ=255)                                                                                    cggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTAt                                                                          >  2:1147484/1‑91 (MQ=255)                                                                                     ggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTAt                                                                          >  2:197642/1‑90 (MQ=255)                                                                                     ggcgAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTAt                                                                          <  1:197642/90‑1 (MQ=255)                                                                                        gAAAGGGCAATACGAACGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGAt                      <  1:787785/139‑1 (MQ=255)                                                                                                        cGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATcc                                                                                                        <  2:62765/41‑1 (MQ=255)                                                                                                        cGTTGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATcc                                                                                                        >  1:62765/1‑41 (MQ=255)                                                                                                          ttGGGTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGt    <  1:1098827/139‑1 (MQ=255)                                                                                                            gggTTATCTTTGTCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTaa  >  2:1280581/1‑139 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATcc                                                                                             >  1:1242112/1‑48 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATcc                                                                                             <  2:1242112/48‑1 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgc                                     >  2:535171/1‑104 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgc                                     <  1:535171/104‑1 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTaa  >  1:296417/1‑139 (MQ=255)                                                                                                            gggTTATCTTTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCCCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTaa  >  1:952185/1‑139 (MQ=255)                                                                                                                     |                                                                                                                                  GAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAGAAGGTAA  >  NC_000913/138270‑138515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑