Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,549,687	A→G	N763D (AAT→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,549,687	0	A	G	100.0%	177.8 / NA	49	N763D (AAT→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/23);  total (26/23)

CATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGA  >  NC_000913/1549573‑1549816                                                                                                                   |                                                                                                                                  cATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           >  1:579753/1‑139 (MQ=255)    cTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGc                                                                                                        >  2:43065/1‑139 (MQ=255)       cGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGc                                                                                                     >  2:71332/1‑139 (MQ=255)               cGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGt                                                                                             >  1:1031531/1‑139 (MQ=255)                ggTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTc                                                                                            <  2:579753/139‑1 (MQ=255)                 gTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  1:432395/1‑115 (MQ=255)                 gTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACGCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  2:432395/115‑1 (MQ=255)                   aCAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTcgcg                                                                                         <  2:1031531/139‑1 (MQ=255)                     aaCCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTg                                                                                       >  1:723233/1‑139 (MQ=255)                          cATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  1:454861/1‑106 (MQ=255)                          cATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  2:454861/106‑1 (MQ=255)                                cttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCg                                                                            <  1:71332/139‑1 (MQ=255)                                cttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCg                                                                            >  1:1447499/1‑139 (MQ=255)                                  tGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTg                                                                          >  2:211522/1‑139 (MQ=255)                                        aTCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTcgg                                                                    >  1:1322548/1‑139 (MQ=255)                                           tACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTcggcgg                                                                 <  1:43065/139‑1 (MQ=255)                                             cacCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGat                                                               <  2:1322548/139‑1 (MQ=255)                                                       tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             >  1:70094/1‑131 (MQ=255)                                                       tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             <  2:70094/131‑1 (MQ=255)                                                       tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTa                                                     >  1:408344/1‑139 (MQ=255)                                                             gagCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               <  1:211522/139‑1 (MQ=255)                                                              agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       <  1:27628/114‑1 (MQ=255)                                                              agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       >  2:27628/1‑114 (MQ=255)                                                              agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTg                                              >  1:178836/1‑139 (MQ=255)                                                                   ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTc                                                                      >  1:707476/1‑110 (MQ=255)                                                                   ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTc                                                                      <  2:707476/110‑1 (MQ=255)                                                                   ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGt                                                      >  1:1406839/1‑126 (MQ=255)                                                                   ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGt                                                      <  2:1406839/126‑1 (MQ=255)                                                                   ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATc                                         >  2:84745/1‑139 (MQ=255)                                                                        ccAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       >  2:934974/1‑104 (MQ=255)                                                                        ccAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       <  1:934974/104‑1 (MQ=255)                                                                               gCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGc                             >  2:547964/1‑139 (MQ=255)                                                                                  aaCCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATcc                                        >  2:629706/1‑125 (MQ=255)                                                                                  aaCCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATcc                                        <  1:629706/125‑1 (MQ=255)                                                                                          tAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTgg                                             >  1:545360/1‑112 (MQ=255)                                                                                          tAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTgg                                             <  2:545360/112‑1 (MQ=255)                                                                                            aCTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACCGATGCTGATCCAGTGGaa                <  2:836376/139‑1 (MQ=255)                                                                                             cTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               <  2:1378535/107‑1 (MQ=255)                                                                                             cTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               >  1:1378535/1‑107 (MQ=255)                                                                                              tCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  2:1165598/1‑38 (MQ=255)                                                                                              tCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  1:1165598/38‑1 (MQ=255)                                                                                               ccGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACgg             <  2:408344/139‑1 (MQ=255)                                                                                                 gACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACg                                  >  2:1216842/1‑116 (MQ=255)                                                                                                 gACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACg                                  <  1:1216842/116‑1 (MQ=255)                                                                                                       tCCGGTCTGGGGGATACTCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             <  2:1030821/83‑1 (MQ=255)                                                                                                       tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           >  1:1295361/1‑37 (MQ=255)                                                                                                       tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           <  2:1295361/37‑1 (MQ=255)                                                                                                       tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             >  1:1030821/1‑83 (MQ=255)                                                                                                          ggTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGa  <  1:547964/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                  CATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGA  >  NC_000913/1549573‑1549816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑