Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,557,112 C→T *309* (TAG→TAA)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,1120CT100.0% 130.2 / NA 41*309* (TAG→TAA) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/19);  total (22/19)

AAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTT  >  NC_000913/1556982‑1557240
                                                                                                                                  |                                                                                                                                
aaagcaaaagcaGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcgc                                                                                                                          >  1:827170/1‑139 (MQ=255)
                            aGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc                                                                                              >  1:130928/1‑139 (MQ=255)
                             ggTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGtcct                                                                                             <  1:660680/139‑1 (MQ=255)
                                aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAg                                                                                                                             >  2:166747/1‑104 (MQ=255)
                                aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAg                                                                                                                             <  1:166747/104‑1 (MQ=255)
                                  tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                              >  2:349096/1‑117 (MQ=255)
                                  tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                              <  1:349096/117‑1 (MQ=255)
                                    aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgt                                                                                                    >  1:1345010/1‑125 (MQ=255)
                                    aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgt                                                                                                    <  2:1345010/125‑1 (MQ=255)
                                        cGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCt                                                                                  >  1:1209686/1‑139 (MQ=255)
                                                          aCCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                                <  2:827170/139‑1 (MQ=255)
                                                            cAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccg                                                                                                 <  1:981688/104‑1 (MQ=255)
                                                            cAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccg                                                                                                 >  2:981688/1‑104 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                           <  1:107684/74‑1 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg                                                                                                                           >  2:107684/1‑74 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                              >  1:865197/1‑87 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg                                                          <  2:703839/139‑1 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg                                                          >  1:703839/1‑139 (MQ=255)
                                                                tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg                                                          >  1:333641/1‑139 (MQ=255)
                                                                tGAAATCGTAAAGCACTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg                                                                                                              <  2:865197/87‑1 (MQ=255)
                                                                 gAAATCGTAAAGCATTGCCGGATGACGCGTCAGGTGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGt                                                         >  2:1373147/1‑139 (MQ=255)
                                                                               ttGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAAt                                                                               >  1:1358941/1‑103 (MQ=255)
                                                                               ttGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAAt                                                                               <  2:1358941/103‑1 (MQ=255)
                                                                                tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGc                                          <  1:1407065/139‑1 (MQ=255)
                                                                                tGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGc                                          <  2:1209686/139‑1 (MQ=255)
                                                                                 gCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa                                         >  1:1509597/1‑139 (MQ=255)
                                                                                       tGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                                >  1:1102302/1‑110 (MQ=255)
                                                                                       tGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca                                                                <  2:1102302/110‑1 (MQ=255)
                                                                                       tGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTc                                   <  2:1509597/139‑1 (MQ=255)
                                                                                            cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg                                                                                                           >  2:628424/1‑62 (MQ=255)
                                                                                            cgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg                                                                                                           <  1:628424/62‑1 (MQ=255)
                                                                                               cAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                       >  1:746993/1‑95 (MQ=255)
                                                                                               cAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCg                                                                       <  2:746993/95‑1 (MQ=255)
                                                                                                       tGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTT‑‑CATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTg                 >  2:1030198/1‑139 (MQ=255)
                                                                                                                gATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAAc          >  2:161259/1‑139 (MQ=255)
                                                                                                                    cGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGc      <  2:333641/139‑1 (MQ=255)
                                                                                                                        gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGc      >  2:417268/1‑135 (MQ=255)
                                                                                                                        gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGc      <  1:417268/135‑1 (MQ=255)
                                                                                                                        gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGtt  >  1:1284738/1‑139 (MQ=255)
                                                                                                                           aGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                               <  2:43672/43‑1 (MQ=255)
                                                                                                                           aGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc                                                                                               >  1:43672/1‑43 (MQ=255)
                                                                                                                                  |                                                                                                                                
AAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTT  >  NC_000913/1556982‑1557240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑