Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,627,625 T→C L37L (TTG→CTG)  ydfG → NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,627,6250TC100.0% 220.9 / NA 61L37L (TTG→CTG) ydfGNADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/33);  total (28/33)

ACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATG  >  NC_000913/1627491‑1627760
                                                                                                                                      |                                                                                                                                       
acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                                     >  1:1193407/1‑139 (MQ=255)
acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                                     >  2:174540/1‑139 (MQ=255)
  acaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAgg                                                                                                                                   <  2:1193407/139‑1 (MQ=255)
      aaGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGtt                                                                                                                               <  1:174540/139‑1 (MQ=255)
        gCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTaa                                                                                                                             <  2:497007/139‑1 (MQ=255)
           ttGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAg                                                                                                                          >  2:1222526/1‑139 (MQ=255)
                      tGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGga                                                                                                               >  1:1461372/1‑139 (MQ=255)
                       gagaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:1461372/139‑1 (MQ=255)
                       gagaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  1:222421/139‑1 (MQ=255)
                         gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            <  2:1223362/139‑1 (MQ=255)
                           tgatCGTTATAGTAACTAGAACAACGGCAGGTTTTGGTTAATGCATTACTCGACGTTTTATTCAACAAGGGAATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATaa                                                                                                          <  2:356660/139‑1 (MQ=255)
                              tCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                                                     <  1:1062271/125‑1 (MQ=255)
                              tCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                                                     >  2:1062271/1‑125 (MQ=255)
                                   ttAGTAACTGGAGCAACGGCAGGGTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCAGtata                                                                                                  >  1:356660/1‑139 (MQ=255)
                                    tAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTatat                                                                                                 >  1:1265336/1‑139 (MQ=255)
                                            ggAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            <  1:399134/120‑1 (MQ=255)
                                            ggAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            >  2:399134/1‑120 (MQ=255)
                                               gCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      <  1:324330/139‑1 (MQ=255)
                                               gCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      <  2:501007/139‑1 (MQ=255)
                                                cAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGa                                                                                     >  2:663714/1‑139 (MQ=255)
                                                 aaCGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGAc                                                                                    <  1:1222526/139‑1 (MQ=255)
                                                    ggCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGtt                                                                                 <  2:1143076/139‑1 (MQ=255)
                                                        ggTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCa                                                                             >  2:1416518/1‑139 (MQ=255)
                                                               gTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                                      <  2:25524/139‑1 (MQ=255)
                                                                tGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgcc                                                                     <  2:123432/139‑1 (MQ=255)
                                                                tGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgcc                                                                     >  1:538566/1‑139 (MQ=255)
                                                                    tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                                    <  2:1331311/72‑1 (MQ=255)
                                                                    tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                                    >  1:1331311/1‑72 (MQ=255)
                                                                    tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGt                                                                                                                                >  1:1337666/1‑76 (MQ=255)
                                                                    tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGt                                                                                                                                <  2:1337666/76‑1 (MQ=255)
                                                                    tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTa                                                                 >  1:1041014/1‑139 (MQ=255)
                                                                               tcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:943531/83‑1 (MQ=255)
                                                                               tcgtTTTATTCAACAAGAGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTACAAGACGAACTGGgag                                                                                                              >  1:943531/1‑83 (MQ=255)
                                                                                cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                                       >  2:970689/1‑73 (MQ=255)
                                                                                cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                                       <  1:970689/73‑1 (MQ=255)
                                                                                        tCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCg                                             >  1:1265570/1‑139 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                                                     <  2:1199925/60‑1 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                                                     >  1:1199925/1‑60 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              >  1:1309975/1‑67 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:1309975/67‑1 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcg                                                                         >  1:1358103/1‑104 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcg                                                                         <  2:1358103/104‑1 (MQ=255)
                                                                                               gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTg                                      >  1:328253/1‑139 (MQ=255)
                                                                                                 gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc                                    <  1:1416518/139‑1 (MQ=255)
                                                                                                    tAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCGGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGAGGCTGGCATCGCTTCCTGCCGAg                                 <  2:1041014/139‑1 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:1340963/56‑1 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  1:858188/56‑1 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              >  1:1340963/1‑56 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              >  2:858188/1‑56 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg                                 >  1:712357/1‑133 (MQ=255)
                                                                                                          tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg                                 <  2:712357/133‑1 (MQ=255)
                                                                                                           aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCa                          <  2:1265336/139‑1 (MQ=255)
                                                                                                                 aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGaa                                                            >  2:850627/1‑99 (MQ=255)
                                                                                                                 aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGaa                                                            <  1:850627/99‑1 (MQ=255)
                                                                                                                    ggCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGata                 <  2:538566/139‑1 (MQ=255)
                                                                                                                     gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcg                                                                         <  1:464648/82‑1 (MQ=255)
                                                                                                                     gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcg                                                                         >  2:464648/1‑82 (MQ=255)
                                                                                                                      ccGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATc               >  1:630642/1‑139 (MQ=255)
                                                                                                                             cAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTg                                                   >  2:887961/1‑96 (MQ=255)
                                                                                                                             cAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTg                                                   <  1:887961/96‑1 (MQ=255)
                                                                                                                                   cGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATg  <  2:630642/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
ACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATG  >  NC_000913/1627491‑1627760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑