Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,935	T→C	K89K (AAA→AAG)	yadV ←	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,935	0	T	C	100.0%	195.3 / NA	56	K89K (AAA→AAG)	yadV	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

TTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155808‑156064                                                                                                                                |                                                                                                                                  tttGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAAt                                                                                                                        >  2:384662/1‑139 (MQ=255)    gATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     <  1:677411/139‑1 (MQ=255)           gACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACt                                                                                                              <  2:8925/139‑1 (MQ=255)                   cTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                      <  2:147664/139‑1 (MQ=255)                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGc                                                                                                                              <  1:370229/107‑1 (MQ=255)                           ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGc                                                                                                                              >  2:370229/1‑107 (MQ=255)                                 ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             >  2:30959/1‑134 (MQ=255)                                 ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                             <  1:30959/134‑1 (MQ=255)                                     gAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    >  2:927753/1‑123 (MQ=255)                                     gAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                    <  1:927753/123‑1 (MQ=255)                                       aCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                                  >  1:382146/1‑139 (MQ=255)                                            cAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAAt                                                                                 <  2:950727/135‑1 (MQ=255)                                            cAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAAt                                                                                 >  1:950727/1‑135 (MQ=255)                                                    tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     >  2:571745/1‑91 (MQ=255)                                                    tAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                     <  1:571745/91‑1 (MQ=255)                                                            aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                      <  2:136958/98‑1 (MQ=255)                                                            aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                      >  1:136958/1‑98 (MQ=255)                                                            aaCACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGt                                                             >  2:1383601/1‑139 (MQ=255)                                                               acGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                          <  1:1491909/139‑1 (MQ=255)                                                                cGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                       <  2:359338/93‑1 (MQ=255)                                                                cGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                       >  1:359338/1‑93 (MQ=255)                                                                  ctctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTGATACTGCCAGGCTCAGCGTTGTCATCGc                                                       >  1:903330/1‑139 (MQ=255)                                                                    tcttctttcttTTGGGAGTGGGGTGCTTGCTTTTTTCATTAATTTGATTGTTTGCCCACCCTTGGCATCAATACGCGATACTGGGGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                     <  2:382146/136‑1 (MQ=255)                                                                    ctctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTGAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCa                                                     <  1:384662/139‑1 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            <  2:845053/130‑1 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            <  2:810158/130‑1 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            >  1:810158/1‑130 (MQ=255)                                                                      ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            >  1:845053/1‑130 (MQ=255)                                                                           tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                 <  2:276107/136‑1 (MQ=255)                                                                           tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                                 >  1:276107/1‑136 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          >  2:119447/1‑93 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                          <  1:119447/93‑1 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            <  1:1470407/139‑1 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            <  1:283614/139‑1 (MQ=255)                                                                             tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCAAGGCTCAGCGTTGTCATCGCCAGTATCTAAc                                            >  2:283614/1‑139 (MQ=255)                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcgg                                                                                                         >  2:370198/1‑76 (MQ=255)                                                                               tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcgg                                                                                                         <  1:370198/76‑1 (MQ=255)                                                                                      tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTg                                                                                     <  1:649805/89‑1 (MQ=255)                                                                                      tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTg                                                                                     >  2:649805/1‑89 (MQ=255)                                                                                        aGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGTGTTGTCATCGCCAGTATCTAACCAACTCTGGAc                                 >  1:367987/1‑139 (MQ=255)                                                                                              tggctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCaa                           >  1:1509393/1‑139 (MQ=255)                                                                                                    tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                     >  1:744922/1‑139 (MQ=255)                                                                                                     gtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                       <  1:651514/56‑1 (MQ=255)                                                                                                     gtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGgcggcg                                                                                                       >  2:651514/1‑56 (MQ=255)                                                                                                          ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGTGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTccc               >  1:605297/1‑139 (MQ=255)                                                                                                             aTTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACCCTGGACAAGCAACGGGTTATTCCCttt            <  2:744922/139‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcggc                                                                                                        >  1:1503858/1‑46 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTggcggc                                                                                                        <  2:1503858/46‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa           <  1:202683/139‑1 (MQ=255)                                                                                                              tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa           >  1:216303/1‑139 (MQ=255)                                                                                                                tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      <  2:502962/94‑1 (MQ=255)                                                                                                                tGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                      >  1:502962/1‑94 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            >  2:895194/1‑86 (MQ=255)                                                                                                                  aTTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTc                                                            <  1:895194/86‑1 (MQ=255)                                                                                                                        tGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  <  2:802398/138‑1 (MQ=255)                                                                                                                        tGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCACCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGa  >  1:802398/1‑138 (MQ=255)                                                                                                                                |                                                                                                                                  TTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGA  >  NC_000913/155808‑156064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑