Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,581	G→A	G295R (GGA→AGA)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,581	0	G	A	100.0%	185.4 / NA	57	G295R (GGA→AGA)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

GGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCT  >  NC_000913/1670452‑1670707                                                                                                                                  |                                                                                                                               gggTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGa                                                                                                                       <  1:588475/139‑1 (MQ=255)                 tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       <  2:906090/139‑1 (MQ=255)                             tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTa                                                                                                             <  2:393846/121‑1 (MQ=255)                             tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTa                                                                                                             >  1:393846/1‑121 (MQ=255)                             tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAt                                                                                           >  2:313051/1‑139 (MQ=255)                                gCTATCGGTTGATGCTCTCCCCCTGGCATGTCCGTCAGGACGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    <  2:1495729/127‑1 (MQ=255)                                gCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    >  1:1495729/1‑127 (MQ=255)                                      ggTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       <  2:158243/118‑1 (MQ=255)                                      ggTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       >  1:158243/1‑118 (MQ=255)                                      ggTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTc                                                                                  <  1:874158/139‑1 (MQ=255)                                                   ccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                     <  1:313051/139‑1 (MQ=255)                                                        ggCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    <  2:87343/103‑1 (MQ=255)                                                        ggCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    >  1:87343/1‑103 (MQ=255)                                                              tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                       <  1:693519/126‑1 (MQ=255)                                                              tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                       >  2:693519/1‑126 (MQ=255)                                                                  tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtg                                                      >  1:1404102/1‑139 (MQ=255)                                                                   cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                     <  1:1258041/139‑1 (MQ=255)                                                                   cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                     >  2:676107/1‑139 (MQ=255)                                                                     ggCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGacca                                                                                                                    <  1:1426139/74‑1 (MQ=255)                                                                     ggCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGacca                                                                                                                    >  2:1426139/1‑74 (MQ=255)                                                                       ccGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGCTTTCGACGGGGTTTATGCTGTTTGGTTTACTGATGAc                                                 >  2:961840/1‑139 (MQ=255)                                                                           ggTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       >  1:849124/1‑81 (MQ=255)                                                                           ggTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       <  2:849124/81‑1 (MQ=255)                                                                                gCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAg                                        >  1:207296/1‑139 (MQ=255)                                                                                     ttatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                   <  2:804253/139‑1 (MQ=255)                                                                                          gCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTgg                              >  2:979267/1‑139 (MQ=255)                                                                                           cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggggg                                                                          >  1:600538/1‑94 (MQ=255)                                                                                           cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggggg                                                                          <  2:600538/94‑1 (MQ=255)                                                                                              gCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCTGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                       <  1:985791/94‑1 (MQ=255)                                                                                              gCTTATTGGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTa                                                                       >  2:985791/1‑94 (MQ=255)                                                                                               cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGAt                         <  2:865470/139‑1 (MQ=255)                                                                                                     tGACCGGCACATGGAGCTCACCCAAAGCCAGAGCCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGGTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGc                   <  2:616014/139‑1 (MQ=255)                                                                                                       aCCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                 <  1:961840/139‑1 (MQ=255)                                                                                                          ggTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTg                                                                  <  2:928803/87‑1 (MQ=255)                                                                                                          ggTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTg                                                                  >  1:928803/1‑87 (MQ=255)                                                                                                                   aGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTg                                                                                                     >  1:1292669/1‑43 (MQ=255)                                                                                                                   aGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTg                                                                                                     <  2:1292669/43‑1 (MQ=255)                                                                                                                   aGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTActct     >  1:315016/1‑139 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       <  1:303648/39‑1 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCg                                                                                                       >  2:303648/1‑39 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                          >  1:19283/1‑52 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                          <  2:19283/52‑1 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTAct       <  2:562513/135‑1 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTAct       >  1:562513/1‑135 (MQ=255)                                                                                                                     cTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttc   >  2:615899/1‑139 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    <  2:643521/41‑1 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTgg                                                                                                    >  1:643521/1‑41 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                  >  1:1019585/1‑43 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                  <  2:1019585/43‑1 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                     >  1:47300/1‑88 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                     <  2:47300/88‑1 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                 <  1:891331/124‑1 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct  >  1:945227/1‑139 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct  <  2:1404102/139‑1 (MQ=255)                                                                                                                      tCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTGTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                 >  2:891331/1‑124 (MQ=255)                                                                                                                             aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                     >  1:1356881/1‑65 (MQ=255)                                                                                                                             aaGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                     <  2:1356881/65‑1 (MQ=255)                                                                                                                                  |                                                                                                                               GGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCT  >  NC_000913/1670452‑1670707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑