Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,023	A→G	F30F (TTT→TTC)	gluQ ←	glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,023	0	A	G	100.0%	204.8 / NA	56	F30F (TTT→TTC)	gluQ	glutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/32);  total (24/32)

CTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NC_000913/159894‑160155                                                                                                                                  |                                                                                                                                     cTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCAGGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGc                                                                                                                             <  2:961987/139‑1 (MQ=255) cTGGCGCAGGACAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGc                                                                                                                             <  1:983571/139‑1 (MQ=255)    gcgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCg                                                                                                                          >  2:580613/1‑139 (MQ=255)     cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGc                                                                                                                         >  2:215085/1‑139 (MQ=255)     cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGc                                                                                                                         <  1:580613/139‑1 (MQ=255)        aGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCgg                                                                                                                      <  1:215085/139‑1 (MQ=255)         ggATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGa                                                                                                                     <  2:713800/139‑1 (MQ=255)                        cACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  1:1304087/139‑1 (MQ=255)                        cACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  2:803789/139‑1 (MQ=255)                          ccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:408303/139‑1 (MQ=255)                          ccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:232517/139‑1 (MQ=255)                          ccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:432464/139‑1 (MQ=255)                          ccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:1510766/139‑1 (MQ=255)                          ccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:631941/139‑1 (MQ=255)                            gggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:764530/139‑1 (MQ=255)                                    cACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCgaaa                                                                                                                                   >  2:1308198/1‑98 (MQ=255)                                    cACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCgaaa                                                                                                                                   <  1:1308198/98‑1 (MQ=255)                                              gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCa                                                                                               <  2:1208942/124‑1 (MQ=255)                                              gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCa                                                                                               >  1:1208942/1‑124 (MQ=255)                                                                 aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:151520/100‑1 (MQ=255)                                                                 aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    >  2:151520/1‑100 (MQ=255)                                                                 aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaa                                                             >  2:61520/1‑139 (MQ=255)                                                                   cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaaa                                                           <  1:61520/139‑1 (MQ=255)                                                                      ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtctg                                                                               >  2:709985/1‑116 (MQ=255)                                                                      ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtctg                                                                               <  1:709985/116‑1 (MQ=255)                                                                        agcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAAtata                                                                                          <  1:404049/103‑1 (MQ=255)                                                                        agcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAAtata                                                                                          >  2:404049/1‑103 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGa                                                                                                                     <  2:1382101/74‑1 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGa                                                                                                                     >  1:1382101/1‑74 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  2:519550/89‑1 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      >  1:519550/1‑89 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                            >  2:199353/1‑99 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                            <  1:199353/99‑1 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAat                                                                                             >  1:788563/1‑98 (MQ=255)                                                                          cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAat                                                                                             <  2:788563/98‑1 (MQ=255)                                                                            aCCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCCGTCATCTCTTTGAACAAAACATAAGGCggg                                                  >  1:225296/1‑139 (MQ=255)                                                                              cTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCa                                                                            >  2:737925/1‑111 (MQ=255)                                                                              cTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCa                                                                            <  1:737925/111‑1 (MQ=255)                                                                              cTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                <  1:879177/139‑1 (MQ=255)                                                                                     ggCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTATCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTcc                                         <  1:1469616/139‑1 (MQ=255)                                                                                       cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    >  1:961508/1‑78 (MQ=255)                                                                                       cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:961508/78‑1 (MQ=255)                                                                                         cgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                     <  2:776151/139‑1 (MQ=255)                                                                                         cgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                     <  1:1164429/139‑1 (MQ=255)                                                                                           aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  2:454596/72‑1 (MQ=255)                                                                                           aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      >  1:454596/1‑72 (MQ=255)                                                                                           aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtg                                   >  2:99014/1‑139 (MQ=255)                                                                                            gCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtgt                                  >  2:162343/1‑139 (MQ=255)                                                                                                      aGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCg                                                                                                                       <  1:807071/44‑1 (MQ=255)                                                                                                      aGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCg                                                                                                                       >  2:807071/1‑44 (MQ=255)                                                                                                      aGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  >  2:1161694/1‑65 (MQ=255)                                                                                                      aGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  1:1161694/65‑1 (MQ=255)                                                                                                      aGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTg                        >  1:559007/1‑139 (MQ=255)                                                                                                            cGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATCAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  >  2:548441/1‑139 (MQ=255)                                                                                                            cGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  >  2:1242755/1‑139 (MQ=255)                                                                                                              aGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACg                >  1:795764/1‑139 (MQ=255)                                                                                                                  cggcgATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTg            >  2:182866/1‑139 (MQ=255)                                                                                                                            agagCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTagcca  <  1:1242755/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NC_000913/159894‑160155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑