Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,721 C→T *335* (TAG→TAA ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,7210CT100.0% 140.9 / NA 44*335* (TAG→TAAldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

CAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGAC  >  NC_000913/1757586‑1757845
                                                                                                                                       |                                                                                                                            
cAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTAc                                                                                                                           <  1:455986/139‑1 (MQ=255)
           aGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                                <  2:826943/139‑1 (MQ=255)
                                    cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatt                                                                                       >  2:842926‑M1/1‑131 (MQ=255)
                                      tCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCT                                                                                               >  1:758898‑M1/1‑129 (MQ=255)
                                      tCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCT                                                                                               <  2:758898‑M1/129‑1 (MQ=255)
                                           acaacaTGGCTACTAAATACCGCATGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                <  1:840679‑M1/139‑16 (MQ=255)
                                                             aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTc                                                                                                      >  2:1459267/1‑99 (MQ=255)
                                                             aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTc                                                                                                      <  1:1459267/99‑1 (MQ=255)
                                                                  aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt                                                                                                   <  1:901610/97‑1 (MQ=255)
                                                                  aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCt                                                                                                   >  2:901610/1‑97 (MQ=255)
                                                                             ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                      <  2:68207/67‑1 (MQ=255)
                                                                             ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGt                                                                                                                      >  1:68207/1‑67 (MQ=255)
                                                                             ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                              >  2:967103/1‑75 (MQ=255)
                                                                             ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTa                                                                                                              <  1:967103/75‑1 (MQ=255)
                                                                              cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagt                                             <  1:842926‑M1/139‑51 (MQ=255)
                                                                              cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggc                                                                 <  1:1359973‑M1/119‑31 (MQ=255)
                                                                              cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggc                                                                 >  2:1359973‑M1/1‑89 (MQ=255)
                                                                               tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc                                                                                 >  2:627757‑M1/1‑88 (MQ=255)
                                                                               tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc                                                                                 <  1:627757‑M1/102‑15 (MQ=255)
                                                                                gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                 >  1:102981/1‑85 (MQ=255)
                                                                                gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                 <  2:102981/85‑1 (MQ=255)
                                                                                gtgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagt                                             >  1:549037‑M1/1‑87 (MQ=255)
                                                                                gtgaagtgaaAAATGGCGCAAATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagt                                             <  2:549037‑M1/137‑51 (MQ=255)
                                                                                 tgaagtgaaAAATGGCGCACATTTTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                 <  1:1097303/84‑1 (MQ=255)
                                                                                 tgaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg                                                                                                 >  2:1097303/1‑84 (MQ=255)
                                                                                      tgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCg                                                                                                                       >  1:532604/1‑57 (MQ=255)
                                                                                      tgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCg                                                                                                                       <  2:532604/57‑1 (MQ=255)
                                                                                        aaaaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                >  1:1093847‑M1/1‑79 (MQ=255)
                                                                                        aaaaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                <  2:1093847‑M1/94‑16 (MQ=255)
                                                                                                cgcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccga                           <  1:729623‑M1/139‑69 (MQ=255)
                                                                                                  cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaa                         >  1:872513‑M1/1‑69 (MQ=255)
                                                                                                       tgtgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccgga                    >  2:1373595‑M1/1‑64 (MQ=255)
                                                                                                       tgtgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                <  2:347485‑M1/79‑16 (MQ=255)
                                                                                                       tgtgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgct                                                                                >  1:347485‑M1/1‑64 (MQ=255)
                                                                                                         tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggata                  >  1:1417589‑M1/1‑62 (MQ=255)
                                                                                                         tgCGCCATTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggata                  >  2:946111‑M1/1‑62 (MQ=255)
                                                                                                               aTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                                >  2:1163431/1‑39 (MQ=255)
                                                                                                               aTTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg                                                                                                                <  1:1163431/39‑1 (MQ=255)
                                                                                                                ttttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcc           <  2:1417589‑M1/139‑85 (MQ=255)
                                                                                                                   tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                               <  1:1156401/36‑1 (MQ=255)
                                                                                                                   tttttGTCTGCCGTTTACCGTTACTGCGTCACGCGt                                                                                                               >  2:1156401/1‑36 (MQ=255)
                                                                                                                         tCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatac  <  2:872513‑M1/139‑94 (MQ=255)
                                                                                                                             ccGTTTACCGTTACTGCGTCACGCGTGACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct                                                         >  2:1037031‑M1/1‑42 (MQ=255)
                                                                                                                             ccGTTTACCGTTACTGCGTCACGCGTGACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct                                                         <  1:1037031‑M1/80‑39 (MQ=255)
                                                                                                                                       |                                                                                                                            
CAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGAC  >  NC_000913/1757586‑1757845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: