Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,301	C→T	A45V (GCG→GTG)	dnaJ →	chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,301	0	C	T	100.0%	159.7 / NA	51	A45V (GCG→GTG)	dnaJ	chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/23);  total (28/23)

ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCA  >  NC_000913/14168‑14431                                                                                                                                      |                                                                                                                                   aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAAt                                                                                                                               >  1:790097/1‑139 (MQ=255)      tAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                                          <  1:1097172/139‑1 (MQ=255)      tAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTaaa                                                                                                                          <  2:494871/139‑1 (MQ=255)               ttattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAg                                                                                                                 <  2:790097/139‑1 (MQ=255)                        gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                        >  2:74104/1‑139 (MQ=255)                         aTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  2:1362608/139‑1 (MQ=255)                            ttAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAg                                                                                                    >  1:1475541/1‑139 (MQ=255)                                               cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                 >  2:415219/1‑139 (MQ=255)                                                           aaaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATa                                                                     >  2:989994/1‑139 (MQ=255)                                                                aGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  1:1236003/100‑1 (MQ=255)                                                                aGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       >  2:1236003/1‑100 (MQ=255)                                                                 gAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCa                                                               >  2:662451/1‑139 (MQ=255)                                                                  aaaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  1:1388177/98‑1 (MQ=255)                                                                  aaaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       >  2:1388177/1‑98 (MQ=255)                                                                         tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTa                                                                                                                            <  1:1071447/70‑1 (MQ=255)                                                                         tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTa                                                                                                                            >  2:1071447/1‑70 (MQ=255)                                                                          aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCa                                                      >  1:537647/1‑139 (MQ=255)                                                                                 ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                                               >  2:395599/1‑75 (MQ=255)                                                                                 ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                                               <  1:395599/75‑1 (MQ=255)                                                                                 ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  1:1255516/83‑1 (MQ=255)                                                                                 ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       >  2:1255516/1‑83 (MQ=255)                                                                                  cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                           >  1:1001409/1‑110 (MQ=255)                                                                                  cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                           <  2:1001409/110‑1 (MQ=255)                                                                                  cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTCATGAAGTTCTGAc                                                                                             >  2:1498744/1‑92 (MQ=255)                                                                                  cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTCATGAAGTTCTGAc                                                                                             <  1:1498744/92‑1 (MQ=255)                                                                                       cATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                         <  2:537647/139‑1 (MQ=255)                                                                                          gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGCAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  2:899573/74‑1 (MQ=255)                                                                                          gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       >  1:899573/1‑74 (MQ=255)                                                                                          gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                 >  1:1381847/1‑112 (MQ=255)                                                                                          gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATGTAAAGAGAGCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                 <  2:1381847/112‑1 (MQ=255)                                                                                           aaaTACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAagag                                                                                                                       >  2:119817/1‑57 (MQ=255)                                                                                           aaaTACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAagag                                                                                                                       <  1:119817/57‑1 (MQ=255)                                                                                                   cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATg                             >  1:657543/1‑139 (MQ=255)                                                                                                       gACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       >  1:331687/1‑61 (MQ=255)                                                                                                       gACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                       <  2:331687/61‑1 (MQ=255)                                                                                                            tAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                    <  2:1475541/139‑1 (MQ=255)                                                                                                             aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGa                                                                  <  2:789130/92‑1 (MQ=255)                                                                                                             aaCCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGa                                                                  >  1:789130/1‑92 (MQ=255)                                                                                                                 aGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                     >  2:1486910/1‑117 (MQ=255)                                                                                                                 aGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                     <  1:1486910/117‑1 (MQ=255)                                                                                                                   ggTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                   >  1:44551/1‑85 (MQ=255)                                                                                                                   ggTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                   <  2:44551/85‑1 (MQ=255)                                                                                                                    gTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGc                                                                                                            <  2:638715/43‑1 (MQ=255)                                                                                                                    gTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGc                                                                                                            >  1:638715/1‑43 (MQ=255)                                                                                                                       aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg         >  1:223852/1‑139 (MQ=255)                                                                                                                             agGCCGAGGTGTAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGgcgc   >  2:644119/1‑139 (MQ=255)                                                                                                                              ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                              <  2:555830/95‑1 (MQ=255)                                                                                                                              ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                              >  1:555830/1‑95 (MQ=255)                                                                                                                              ggCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa  >  2:524687/1‑139 (MQ=255)                                                                                                                                 cGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                            >  1:861419/1‑62 (MQ=255)                                                                                                                                 cGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                            <  2:861419/62‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCA  >  NC_000913/14168‑14431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑