Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,172	C→T	*327* (TAG→TAA)	btuC ←	vitamin B12 ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,172	0	C	T	100.0%	163.9 / NA	52	*327* (TAG→TAA)	btuC	vitamin B12 ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/25);  total (27/25)

CGGCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCC  >  NC_000913/1794037‑1794305                                                                                                                                        |                                                                                                                                      cGGCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTaa                                                                                                                                    >  2:1432642/1‑139 (MQ=255)   gCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACg                                                                                                                                  >  2:1279385/1‑139 (MQ=255)     gAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTc                                                                                                                                <  2:717873/139‑1 (MQ=255)       aCTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCt                                                                                                                              <  1:416993/139‑1 (MQ=255)            tcCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCAGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                         <  2:156100/139‑1 (MQ=255)                    ggTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                                 <  1:247932/139‑1 (MQ=255)                      tCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAAc                                                                                                               >  1:36328/1‑139 (MQ=255)                                          ttCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCAcc                                                                                           <  2:36328/139‑1 (MQ=255)                                           tCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                         >  2:262404/1‑108 (MQ=255)                                           tCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                         <  1:262404/108‑1 (MQ=255)                                               tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                                 >  1:869401/1‑112 (MQ=255)                                               tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                                 <  2:869401/112‑1 (MQ=255)                                               tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      <  2:1088214/139‑1 (MQ=255)                                                   gtcgtcAGAATGGAATCTTGCATCGTTTCTCCTGGGGGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTACAGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGc                                                                                  >  1:1455405/1‑139 (MQ=255)                                                                aaTCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                         >  2:484958/1‑87 (MQ=255)                                                                aaTCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                         <  1:484958/87‑1 (MQ=255)                                                                  tCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                                 >  1:1215278/1‑125 (MQ=255)                                                                  tCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                                 <  2:1215278/125‑1 (MQ=255)                                                                     tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTc                                                                                                                                <  2:776428/75‑1 (MQ=255)                                                                     tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTc                                                                                                                                >  1:776428/1‑75 (MQ=255)                                                                     tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      >  2:1218219/1‑117 (MQ=255)                                                                     tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      <  1:1218219/117‑1 (MQ=255)                                                                     tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAgg                                                                >  2:631032/1‑139 (MQ=255)                                                                                tcCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          <  2:679697/118‑1 (MQ=255)                                                                                tcCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          >  1:1360805/1‑118 (MQ=255)                                                                                tcCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          <  2:1360805/118‑1 (MQ=255)                                                                                tcCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          >  1:679697/1‑118 (MQ=255)                                                                                   tGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAg                                                  <  1:1279385/139‑1 (MQ=255)                                                                                       tgtgGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTGCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      <  1:983804/99‑1 (MQ=255)                                                                                       tgtgGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      >  2:983804/1‑99 (MQ=255)                                                                                          ggTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGc                                           >  2:799457/1‑139 (MQ=255)                                                                                                tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTg                                                                                                                             >  1:586744/1‑51 (MQ=255)                                                                                                tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTg                                                                                                                             <  2:586744/51‑1 (MQ=255)                                                                                                          aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      >  2:774488/1‑80 (MQ=255)                                                                                                          aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                      <  1:774488/80‑1 (MQ=255)                                                                                                                         aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt            >  2:323645/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGgc                                                                                                <  1:408457/51‑1 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGgc                                                                                                >  2:408457/1‑51 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          >  2:781679/1‑73 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          <  1:781679/73‑1 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          >  2:421936/1‑73 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                          <  1:421936/73‑1 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGTCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        >  1:768557/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAatat                          >  1:989029/1‑121 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAatat                          <  2:989029/121‑1 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        >  2:49949/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        >  1:1472069/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        <  1:49949/139‑1 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        >  2:1321041/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        >  1:1237624/1‑139 (MQ=255)                                                                                                                             tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACATAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc        <  1:799457/139‑1 (MQ=255)                                                                                                                                   tGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGccc  <  1:323645/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CGGCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCC  >  NC_000913/1794037‑1794305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑