Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,819,569 A→G G79G (GGT→GGC chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,5690AG100.0% 203.1 / NA 56G79G (GGT→GGCchbAN,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

TGGCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGC  >  NC_000913/1819436‑1819703
                                                                                                                                     |                                                                                                                                      
tGGCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTc                                                                                                                                   >  2:1236240/1‑139 (MQ=255)
 ggCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCg                                                                                                                                  <  1:1236240/139‑1 (MQ=255)
        tcatcGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  2:314606/139‑1 (MQ=255)
          atcGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCg                                                                                                                         <  1:989170/139‑1 (MQ=255)
                   ttATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATc                                                                                                                >  1:605563/1‑139 (MQ=255)
                     aTGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAg                                                                                                              >  2:1069907/1‑139 (MQ=255)
                               tttttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           >  1:31424/1‑116 (MQ=255)
                               tttttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  2:31424/116‑1 (MQ=255)
                                 tttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTAc                                                                                                  <  2:605563/139‑1 (MQ=255)
                                                    ttCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             >  1:1281603/1‑125 (MQ=255)
                                                    ttCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                             <  2:1281603/125‑1 (MQ=255)
                                                     tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCt                                                                                                                      <  1:29478/99‑1 (MQ=255)
                                                     tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCt                                                                                                                      >  2:29478/1‑99 (MQ=255)
                                                        gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  2:1325432/91‑1 (MQ=255)
                                                        gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           >  1:1325432/1‑91 (MQ=255)
                                                        gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCa                                                                           >  1:681465/1‑139 (MQ=255)
                                                        gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCa                                                                           >  1:577377/1‑139 (MQ=255)
                                                         tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCAt                                                                          >  2:409616/1‑139 (MQ=255)
                                                                   aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                            <  1:948110/95‑1 (MQ=255)
                                                                   aCGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                            >  2:948110/1‑95 (MQ=255)
                                                                    cgcgCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGAt                                                               <  1:1069907/139‑1 (MQ=255)
                                                                        cAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcat                                                           >  2:590554/1‑139 (MQ=255)
                                                                         aaGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGCACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatc                                                          <  1:985999/139‑1 (MQ=255)
                                                                          aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           >  2:1235274/1‑73 (MQ=255)
                                                                          aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  1:1235274/73‑1 (MQ=255)
                                                                          aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTTTACCAGATGCCCTTCATTCAATCCCATCGGTGACTGATCcatca                                                         >  2:249754/1‑139 (MQ=255)
                                                                          aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatca                                                         >  1:1415718/1‑139 (MQ=255)
                                                                                   gTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtg                                                <  1:409616/139‑1 (MQ=255)
                                                                                         aaaTGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCgg                                          <  1:249754/139‑1 (MQ=255)
                                                                                               tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           <  1:313669/52‑1 (MQ=255)
                                                                                               tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                           >  2:313669/1‑52 (MQ=255)
                                                                                               tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgc                                               >  1:267591/1‑128 (MQ=255)
                                                                                               tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgc                                               <  2:267591/128‑1 (MQ=255)
                                                                                               tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                    >  2:824136/1‑139 (MQ=255)
                                                                                                ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATc                                   >  2:164466/1‑139 (MQ=255)
                                                                                                   gAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGcc                                <  1:164466/139‑1 (MQ=255)
                                                                                                      cGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTg                             >  1:1007112/1‑139 (MQ=255)
                                                                                                        tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                           >  1:729507/1‑139 (MQ=255)
                                                                                                         ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTg                                                                 <  1:135844/100‑1 (MQ=255)
                                                                                                         ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTg                                                                 >  2:135844/1‑100 (MQ=255)
                                                                                                         ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                          >  1:1449897/1‑139 (MQ=255)
                                                                                                         ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAACAGTTTCGTCTGTACCAGATGTGCGTCATTCAATGCCATCCGTGACTCATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                          >  1:923040/1‑139 (MQ=255)
                                                                                                          gACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATcc                                                                        >  2:1446256/1‑92 (MQ=255)
                                                                                                          gACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATcc                                                                        <  1:1446256/92‑1 (MQ=255)
                                                                                                              aGCACCAGACTCACTTTCATCTTGCCTTCGTCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCt                     >  2:805994/1‑139 (MQ=255)
                                                                                                              aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCGGTACCAGATGCGCt                                                                                        <  1:617896/72‑1 (MQ=255)
                                                                                                              aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCGGTACCAGATGCGCt                                                                                        >  2:617896/1‑72 (MQ=255)
                                                                                                                cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGt                   >  2:1191789/1‑139 (MQ=255)
                                                                                                                  ccAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGttt                 <  2:681465/139‑1 (MQ=255)
                                                                                                                   cAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                       >  2:625907/1‑52 (MQ=255)
                                                                                                                   cAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                       <  1:625907/52‑1 (MQ=255)
                                                                                                                     gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCt                                                                                                                      >  2:1138225/1‑35 (MQ=255)
                                                                                                                     gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCt                                                                                                                      <  1:1138225/35‑1 (MQ=38)
                                                                                                                       cTCACTTTCATCTTGCCTTCGTCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTg            <  1:805994/139‑1 (MQ=255)
                                                                                                                           cTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGc        <  1:1191789/139‑1 (MQ=255)
                                                                                                                                 tCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGc  <  2:577377/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TGGCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACGC  >  NC_000913/1819436‑1819703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: