Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	167,743	C→T	T87I (ACC→ATC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	167,743	0	C	T	100.0%	151.6 / NA	47	T87I (ACC→ATC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

CACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167609‑167877                                                                                                                                       |                                                                                                                                       cACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTA‑CGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGAtcgccg                                                                                                                                   >  2:1396280/1‑139 (MQ=255)             tGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        <  2:761037/139‑1 (MQ=255)             tGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGgcg                                                                                                                        <  1:445502/139‑1 (MQ=255)                         gcAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAGAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCg                                                                                                            >  2:985665/1‑139 (MQ=255)                         gcAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCg                                                                                                            >  1:1108578/1‑139 (MQ=255)                         gcAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCg                                                                                                            <  2:1056939/139‑1 (MQ=255)                           aaGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTA‑CGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAg                                                                                                         <  1:1396280/139‑1 (MQ=255)                                         gggCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAaagaag                                                                                            <  2:1108578/139‑1 (MQ=255)                                            ccTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAgcgc                                                                                         >  2:1506782/1‑139 (MQ=255)                                                      cTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTacac                                                                               >  2:27646/1‑139 (MQ=255)                                                             ggcgCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgt                                                                        >  2:1210956/1‑139 (MQ=255)                                                               cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          <  1:267364/103‑1 (MQ=255)                                                               cgcgACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          >  2:267364/1‑103 (MQ=255)                                                                              ccGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGAt                                                                                                                            >  2:598201/1‑70 (MQ=255)                                                                              ccGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGAt                                                                                                                            <  1:598201/70‑1 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTTAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTg                                                     <  1:842493/138‑1 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTgg                                                    >  1:1481369/1‑139 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTgg                                                    >  1:859021/1‑139 (MQ=255)                                                                                 gCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTg                                                     >  2:842493/1‑138 (MQ=255)                                                                                  cACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgc                                                   <  1:1506782/139‑1 (MQ=255)                                                                                   aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCa                                                                                                                   >  1:353210/1‑74 (MQ=255)                                                                                   aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCa                                                                                                                   <  2:353210/74‑1 (MQ=255)                                                                                   aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgcg                                                  >  2:263325/1‑139 (MQ=255)                                                                                   aCTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGgcg                                                  >  2:1355769/1‑139 (MQ=255)                                                                                         aCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            <  1:243398/139‑1 (MQ=255)                                                                                         aCCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCa                                            <  1:1355769/139‑1 (MQ=255)                                                                                          ccGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                           <  1:985665/139‑1 (MQ=255)                                                                                                  ccGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATg                                   <  2:55384/139‑1 (MQ=255)                                                                                                   cGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGtct                                                                    >  2:1023831/1‑105 (MQ=255)                                                                                                   cGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCCTAGCTACACGCCGGGTGtct                                                                    <  1:1023831/105‑1 (MQ=255)                                                                                                     aTTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          >  2:467697/1‑65 (MQ=255)                                                                                                     aTTCAAAAAGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGaa                                                                                                          <  1:467697/65‑1 (MQ=255)                                                                                                             aGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATc                                              >  1:1014742/1‑117 (MQ=255)                                                                                                             aGTGCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATc                                              <  2:1014742/117‑1 (MQ=255)                                                                                                                gCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCa                                                                                                                   >  2:695864/1‑45 (MQ=255)                                                                                                                gCCACAGTCTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCa                                                                                                                   <  1:695864/45‑1 (MQ=255)                                                                                                                        cTATTTCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCtt             >  1:1172726/1‑139 (MQ=255)                                                                                                                           tttCTGTTGTGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGc          <  1:27646/139‑1 (MQ=255)                                                                                                                                gttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAg     <  2:1470307/139‑1 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCTGGTGTCTCTGTTGGTACGCGTGGCGCa                                                <  2:1466830/95‑1 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      <  1:512457/73‑1 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGgtgt                                                                      >  2:512457/1‑73 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCa                                                >  1:1466830/1‑95 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                           <  2:1349653/100‑1 (MQ=255)                                                                                                                                 ttgtGATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCaa                                           >  1:1349653/1‑100 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTAt                                    <  1:1187289/105‑1 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTAt                                    >  2:1187289/1‑105 (MQ=255)                                                                                                                                   gtgATCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAg  >  2:65538/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       CACCGTTACCGCTGCACCTGCGCCGCAAGAAAGCGCATGGGGGCCTGCTGCAACTATTGCGGCGCGACAGTCTGCTACCGGCACTAAAACCGATACGCCGATTCAAAAAGTGCCACAGTCTATTTCTGTTGTGACCGCCGAAGAGATGGCGCTGCATCAGCCGAAGTCGGTAAAAGAAGCGCTTAGCTACACGCCGGGTGTCTCTGTTGGTACGCGTGGCGCATCCAACACCTATGACCACCTGATCATTCGCGGCTTTGCGGCAGAAG  >  NC_000913/167609‑167877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑