Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,810	G→A	*322* (TAG→TAA)	yeaX →	putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,810	0	G	A	100.0%	150.6 / NA	48	*322* (TAG→TAA)	yeaX	putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

CCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG  >  NC_000913/1886674‑1886935                                                                                                                                         |                                                                                                                              ccTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAAtt                                                                                                                             <  2:384586/139‑1 (MQ=255) ccTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAAtt                                                                                                                             >  2:634453/1‑139 (MQ=255)                          tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                   >  2:496897/1‑124 (MQ=255)                          tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                   <  1:496897/124‑1 (MQ=255)                          tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                    >  1:1111604/1‑139 (MQ=255)                          tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                    >  2:141558/1‑139 (MQ=255)                          tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGAGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                    >  2:1264337/1‑139 (MQ=255)                                             cAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                 <  1:141558/139‑1 (MQ=255)                                              aaTATTTTAGCGATGAAGAGCGTGCCAGCGAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCgg                                                                                >  2:1193505/1‑139 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          >  2:622921/1‑123 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          <  1:622921/123‑1 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTc                                                                                         >  2:1293923/1‑124 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTc                                                                                         <  1:930483/124‑1 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTc                                                                                         >  2:930483/1‑124 (MQ=255)                                                    ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTc                                                                                         <  1:1293923/124‑1 (MQ=255)                                                     tAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          <  1:269272/122‑1 (MQ=255)                                                     tAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          >  2:269272/1‑122 (MQ=255)                                                         gATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                     <  1:1264337/139‑1 (MQ=255)                                                          aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                              >  1:37031/1‑113 (MQ=255)                                                          aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                              <  2:37031/113‑1 (MQ=255)                                                                    tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          <  2:88033/107‑1 (MQ=255)                                                                    tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          >  1:88033/1‑107 (MQ=255)                                                                       cagccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACG‑CTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                      >  1:859583/1‑139 (MQ=255)                                                                        agccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                      <  2:500099/139‑1 (MQ=255)                                                                                        tGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGc                                      >  1:785298/1‑139 (MQ=255)                                                                                            cTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTcc                                  <  2:785298/139‑1 (MQ=255)                                                                                             tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                    <  2:584807/72‑1 (MQ=255)                                                                                             tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                    >  1:584807/1‑72 (MQ=255)                                                                                               ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                        <  1:1481556/66‑1 (MQ=255)                                                                                               ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                        >  2:1481556/1‑66 (MQ=255)                                                                                                 gttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                             >  2:304859/1‑139 (MQ=255)                                                                                                 gttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                             <  1:1193505/139‑1 (MQ=255)                                                                                                 gttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                             <  2:1111604/139‑1 (MQ=255)                                                                                                 gttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                             >  1:803181/1‑139 (MQ=255)                                                                                                    cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAACCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                          >  1:1406319/1‑139 (MQ=255)                                                                                                     gcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGc                         >  1:921757/1‑139 (MQ=255)                                                                                                        tGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTca                      <  1:304859/139‑1 (MQ=255)                                                                                                         gCGAAGGGTAACCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTcc                                                                                        <  2:1291739/72‑1 (MQ=255)                                                                                                         gCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTcc                                                                                        >  1:1291739/1‑72 (MQ=255)                                                                                                         gCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcac                     <  2:803181/139‑1 (MQ=255)                                                                                                          cGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcacc                    >  2:954102/1‑139 (MQ=255)                                                                                                              gggTAAACG‑CTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGcc               <  2:859583/139‑1 (MQ=255)                                                                                                                  aaaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc            <  1:634453/139‑1 (MQ=255)                                                                                                                   aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                       >  2:1418114/1‑79 (MQ=255)                                                                                                                   aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                       <  1:1418114/79‑1 (MQ=255)                                                                                                                    aCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCg          >  1:1203128/1‑139 (MQ=255)                                                                                                                           gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCa   >  2:355567/1‑139 (MQ=255)                                                                                                                            tgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAg  <  2:1203128/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                              CCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG  >  NC_000913/1886674‑1886935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑