Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,993	G→A	G111E (GGG→GAG)	znuC →	zinc ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,993	0	G	A	100.0%	202.3 / NA	62	G111E (GGG→GAG)	znuC	zinc ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

CGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCA  >  NC_000913/1942863‑1943128                                                                                                                                   |                                                                                                                                        cgcATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGa                                                                                                                                 >  1:448197/1‑139 (MQ=255)  gcATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGAt                                                                                                                                <  1:234392/139‑1 (MQ=255)               aCCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATg                                                                                                                   <  2:453241/139‑1 (MQ=255)                   cAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaaa                                                                                                               >  2:978345/1‑139 (MQ=255)                        gCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGct                                                                                                           <  2:96882/138‑1 (MQ=255)                        gCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGct                                                                                                           >  1:96882/1‑138 (MQ=255)                                      ccacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAAc                                                                                            >  1:314748/1‑139 (MQ=255)                                        acGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                                          <  1:638674/139‑1 (MQ=255)                                              ccACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                                    <  2:448197/139‑1 (MQ=255)                                                 cTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTAc                                                                                 >  1:282657/1‑139 (MQ=255)                                                        tAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGa                                                                                                                     >  2:961295/1‑96 (MQ=255)                                                        tAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGa                                                                                                                     <  1:961295/96‑1 (MQ=255)                                                        tAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAgc                                                                          >  2:83627/1‑139 (MQ=255)                                                             cGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     <  1:825599/139‑1 (MQ=255)                                                             cGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     <  1:978345/139‑1 (MQ=255)                                                                      cgcttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACg                                                                                                                           <  2:240346/76‑1 (MQ=255)                                                                      cgcttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACg                                                                                                                           >  1:240346/1‑76 (MQ=255)                                                                            cgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGcc                                                      <  1:83627/139‑1 (MQ=255)                                                                                   gTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctctc                                                                                                        >  2:984555/1‑82 (MQ=255)                                                                                   gTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctctc                                                                                                        <  1:984555/82‑1 (MQ=255)                                                                                        caTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           >  1:215694/1‑90 (MQ=255)                                                                                        caTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           <  2:215694/90‑1 (MQ=255)                                                                                        caTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCa                                                                   >  1:703113/1‑114 (MQ=255)                                                                                        caTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCa                                                                   <  2:703113/114‑1 (MQ=255)                                                                                         atgaagaagATCTTTTGCCTGCACTGAAACGTGTCCAGGCCGAACACCTGATAAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                         >  2:873687/4‑91 (MQ=255)                                                                                         aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAACACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                         <  1:873687/91‑1 (MQ=255)                                                                                         aTAAAGAAGATATTTTGCC‑GCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCg                                                    >  1:1319638/1‑127 (MQ=255)                                                                                         aTAAAGAAGATATTTTGCC‑GCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCg                                                    <  2:1319638/127‑1 (MQ=255)                                                                                           aaaGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     <  1:556731/109‑1 (MQ=255)                                                                                           aaaGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     >  2:556731/1‑109 (MQ=255)                                                                                             agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTg                                     >  2:677285/1‑139 (MQ=255)                                                                                              gaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCt                                                                                                                                   >  2:948964/1‑44 (MQ=255)                                                                                              gaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCt                                                                                                                                   <  1:948964/44‑1 (MQ=255)                                                                                                     ttttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                        <  1:714154/80‑1 (MQ=255)                                                                                                     ttttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                        >  2:714154/1‑80 (MQ=255)                                                                                                         gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGc                                       >  1:1428078/1‑125 (MQ=255)                                                                                                         gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGc                                       <  2:1428078/125‑1 (MQ=255)                                                                                                                 tGAAACGAATCCAGGCCGAGCACCTGATTAACGAACCTATGAAAAAGCTCACTGGAGACGAAACGCAGAGTGTACTATGAGCGCGGGCATTGTTCAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGt                 <  2:314748/139‑1 (MQ=255)                                                                                                                   aaaCGTGTCCAGGCCGAGTACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     <  1:444492/85‑1 (MQ=255)                                                                                                                   aaaCGTGTCCAGGCCGAGTACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                                     >  2:444492/1‑85 (MQ=255)                                                                                                                   aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           <  2:1315503/63‑1 (MQ=255)                                                                                                                   aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           >  1:1315503/1‑63 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAAGGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTa          >  2:1022582/1‑139 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           <  2:145738/58‑1 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                           >  1:145738/1‑58 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg                                                                                      >  1:925389/1‑63 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg                                                                                      <  2:925389/63‑1 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCa                                                                   <  2:872350/82‑1 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCa                                                                   >  1:872350/1‑82 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCg                                                    <  1:312257/97‑1 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCg                                                    >  2:312257/1‑97 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTg                                        <  2:520346/109‑1 (MQ=255)                                                                                                                        tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTg                                        >  1:520346/1‑109 (MQ=255)                                                                                                                           ccAGGCCGAGCACCTGATTAACGCACCGATGCCAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATAGc                                                       >  2:3461/1‑91 (MQ=255)                                                                                                                           ccAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                                       <  1:3461/91‑1 (MQ=255)                                                                                                                             aGGCCGAGCACCTGATTAACGCACCGATGCAAAAGct                                                                                                           >  2:572974/1‑37 (MQ=255)                                                                                                                             aGGCCGAGCACCTGATTAACGCACCGATGCAAAAGct                                                                                                           <  1:572974/37‑1 (MQ=255)                                                                                                                             aGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                         <  1:691274/55‑1 (MQ=255)                                                                                                                             aGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                         >  2:691274/1‑55 (MQ=255)                                                                                                                                ccGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCa  >  1:261706/1‑139 (MQ=255)                                                                                                                                ccGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTA‑TAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                                   >  1:615687/1‑105 (MQ=255)                                                                                                                                ccGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTA‑TAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                                   <  2:615687/105‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCA  >  NC_000913/1942863‑1943128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑