Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,947,567	G→A	S195S (AGC→AGT)	yebC ←	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,947,567	0	G	A	100.0%	221.8 / NA	69	S195S (AGC→AGT)	yebC	UPF0082 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/36);  total (33/36)

CAGAGATTTCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATG  >  NC_000913/1947434‑1947702                                                                                                                                      |                                                                                                                                        caGAGATTTCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGGCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTc                                                                                                                                    <  1:276523/139‑1 (MQ=255)  agagATTTCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCt                                                                                                                                   <  2:410385/139‑1 (MQ=255)     gaTTTCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCt                                                                                                                                >  1:1115937/1‑139 (MQ=255)       tttCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCttt                                                                                                                              <  1:152854/139‑1 (MQ=255)         tCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTc                                                                                                                                    >  1:112579/1‑131 (MQ=255)         tCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTc                                                                                                                                    <  2:112579/131‑1 (MQ=255)          cACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAg                                                                                                                           >  2:988084/1‑139 (MQ=255)                          ttCCTGCAGGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCaga                                                                                                           <  2:918210/139‑1 (MQ=255)                          ttCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCaga                                                                                                           <  1:1372794/139‑1 (MQ=255)                                    tcgtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGAc                                                                                                                         >  1:1040683/1‑115 (MQ=255)                                    tcgtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGAc                                                                                                                         <  2:1040683/115‑1 (MQ=255)                                       tcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACtt                                                                                              >  2:662584/1‑139 (MQ=255)                                               ttCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCAtt                                                                                      >  1:56788/1‑139 (MQ=255)                                                    gCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATttcttc                                                                                 <  2:1125544/139‑1 (MQ=255)                                                      atatCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTccc                                                                               <  1:336146/139‑1 (MQ=255)                                                         tCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                            <  2:1104394/139‑1 (MQ=255)                                                            aTCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     >  1:29748/1‑95 (MQ=255)                                                            aTCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     <  2:29748/95‑1 (MQ=255)                                                                 aCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     >  2:286076/1‑90 (MQ=255)                                                                 aCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     <  1:286076/90‑1 (MQ=255)                                                                 aCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCa                                                                                                             <  2:774700/98‑1 (MQ=255)                                                                 aCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCa                                                                                                             >  1:774700/1‑98 (MQ=255)                                                                    cATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGAGCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACa                                                                 >  2:1128396/1‑139 (MQ=255)                                                                     aTCAGTTTCGGTGCGGTTTTTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCagag                                                                                                          <  1:531652/97‑1 (MQ=255)                                                                     aTCAGTTTCGGTGCGGTTTTTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCagag                                                                                                          >  2:531652/1‑97 (MQ=255)                                                                      tCAGTTTCGGTGCGGTTTCTGCATCCATACCAGCTTTGGTAGACGGAATCATGGGAACTTCCGCACTATCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATc                                                               >  2:80034/1‑139 (MQ=255)                                                                           ttCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCAt                                                                             <  2:1181683/120‑1 (MQ=255)                                                                           ttCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCAt                                                                             >  1:1181683/1‑120 (MQ=255)                                                                             cGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATcgcg                                                        <  2:1115937/139‑1 (MQ=255)                                                                               gTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     >  2:952314/1‑76 (MQ=255)                                                                               gTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     <  1:952314/76‑1 (MQ=255)                                                                                tGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCAtt                                                                                      <  1:1144987/106‑1 (MQ=255)                                                                                tGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCAtt                                                                                      >  2:1144987/1‑106 (MQ=255)                                                                                    gTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCagag                                                                                                          <  1:827215/82‑1 (MQ=255)                                                                                    gTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCagag                                                                                                          >  2:827215/1‑82 (MQ=255)                                                                                               cATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTcaca                                      <  1:125092/139‑1 (MQ=255)                                                                                                  atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACg                                   >  2:743023/1‑139 (MQ=255)                                                                                                          tGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTcgc                                                                                                    <  2:551274/66‑1 (MQ=255)                                                                                                          tGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTcgc                                                                                                    >  1:551274/1‑66 (MQ=255)                                                                                                          tGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTcac                                       <  2:316653/127‑1 (MQ=255)                                                                                                          tGGTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTcac                                       >  1:316653/1‑127 (MQ=255)                                                                                                           ggTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     <  2:510351/48‑1 (MQ=255)                                                                                                           ggTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGc                                                                                                                     >  1:510351/1‑48 (MQ=255)                                                                                                           ggTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCa                          <  1:988084/139‑1 (MQ=255)                                                                                                           ggTAGACGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCa                          >  2:259764/1‑139 (MQ=255)                                                                                                                 cGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGc                                                                                                                  >  1:168907/1‑45 (MQ=255)                                                                                                                 cGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGc                                                                                                                  <  2:168907/45‑1 (MQ=255)                                                                                                                 cGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCt                    >  1:1031011/1‑139 (MQ=255)                                                                                                                 cGGGATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCt                    >  2:1410394/1‑139 (MQ=255)                                                                                                                   ggATCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTc                  <  1:662584/139‑1 (MQ=255)                                                                                                                     aTCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGTACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                      <  2:1065206/101‑1 (MQ=255)                                                                                                                     aTCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                      >  1:1065206/1‑101 (MQ=255)                                                                                                                      tCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGc                                                                                                                  >  2:1073257/1‑40 (MQ=255)                                                                                                                      tCATGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGc                                                                                                                  <  1:1073257/40‑1 (MQ=39)                                                                                                                         tGGAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCAACATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgc            >  1:385922/1‑139 (MQ=255)                                                                                                                          ggAAACTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCAACATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgct           <  2:385922/139‑1 (MQ=255)                                                                                                                            aaaCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAAt                                                            >  1:167581/1‑88 (MQ=255)                                                                                                                            aaaCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAAt                                                            <  2:167581/88‑1 (MQ=255)                                                                                                                            aaaCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctgc         <  2:56788/139‑1 (MQ=255)                                                                                                                             aaCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTgctgct        <  1:80034/139‑1 (MQ=255)                                                                                                                              aCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCgcgc                                                       <  2:326222/91‑1 (MQ=255)                                                                                                                              aCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCgcgc                                                       >  1:326222/1‑91 (MQ=255)                                                                                                                              aCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCa                             >  2:542771/1‑117 (MQ=255)                                                                                                                              aCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCa                             <  1:542771/117‑1 (MQ=255)                                                                                                                              aCTTCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt       >  2:132683/1‑139 (MQ=255)                                                                                                                                ttCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTcgc                                                                                                    >  2:43074/1‑44 (MQ=255)                                                                                                                                ttCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTcgc                                                                                                    <  1:43074/44‑1 (MQ=255)                                                                                                                                 tCCGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCa    <  1:132683/139‑1 (MQ=255)                                                                                                                                  ccGCACTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCat   >  2:263256/1‑139 (MQ=255)                                                                                                                                   cgcaCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg  <  2:1031011/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CAGAGATTTCACCGTTATGGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATG  >  NC_000913/1947434‑1947702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑