Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,662	C→T	*267* (TAG→TAA)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,662	0	C	T	100.0%	190.3 / NA	58	*267* (TAG→TAA)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

AGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTA  >  NC_000913/177527‑177784                                                                                                                                        |                                                                                                                           aGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTaa                                                                                                                         >  1:608772/1‑139 (MQ=255)  gAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:1248989/139‑1 (MQ=255)  gAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:137583/139‑1 (MQ=255)       ccAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTAcc                                                                                                                   >  1:748758/1‑139 (MQ=255)       ccAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTAcc                                                                                                                   >  2:1302788/1‑139 (MQ=255)       ccAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTAcc                                                                                                                   >  2:463939/1‑139 (MQ=255)        cAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCt                                                                                                                  >  1:574177/1‑139 (MQ=255)                tCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgc                                                                                                          >  2:1322515/1‑139 (MQ=255)                  cATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGa                                                                                                              <  2:352401/133‑1 (MQ=255)                  cATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGa                                                                                                              >  1:352401/1‑133 (MQ=255)                        cAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACa                                                                                                  >  2:1025299/1‑139 (MQ=255)                           ttttCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               >  1:1279593/1‑139 (MQ=255)                           ttttCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTAGATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               <  1:956461/139‑1 (MQ=255)                              tCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCtg                                                                                            >  2:393190/1‑139 (MQ=255)                                     aaCAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCa                                                                                     <  1:1302788/139‑1 (MQ=255)                                               cGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGataa                                                                           >  1:480617/1‑139 (MQ=255)                                                 gagaTGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGataata                                                                         >  1:722050/1‑139 (MQ=255)                                                            tACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  >  2:348341/1‑119 (MQ=255)                                                            tACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                                  <  1:348341/119‑1 (MQ=255)                                                            tACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                              <  1:463939/139‑1 (MQ=255)                                                            tACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                              <  2:574177/139‑1 (MQ=255)                                                             aCCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCa                                                             <  1:1322515/139‑1 (MQ=255)                                                                 cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        <  1:1271121/76‑1 (MQ=255)                                                                 cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAAt                                                                                                                        >  2:1271121/1‑76 (MQ=255)                                                                 cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                         <  2:989349/91‑1 (MQ=255)                                                                 cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                         >  1:989349/1‑91 (MQ=255)                                                                       cGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAAcc                                                   <  1:393190/139‑1 (MQ=255)                                                                         aCTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAg                                                 <  2:722050/139‑1 (MQ=255)                                                                               tCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTc                                           <  2:748758/139‑1 (MQ=255)                                                                                    ccAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACg                                                                       <  1:119309/106‑1 (MQ=255)                                                                                    ccAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACg                                                                       >  2:119309/1‑106 (MQ=255)                                                                                     cAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                              <  1:1123103/114‑1 (MQ=255)                                                                                     cAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                              >  2:1123103/1‑114 (MQ=255)                                                                                        tAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgaga                                     >  2:380971/1‑136 (MQ=255)                                                                                        tAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgaga                                     <  1:380971/136‑1 (MQ=255)                                                                                        tAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGa                                  >  1:928723/1‑139 (MQ=255)                                                                                                         aTCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                 >  2:1121525/1‑139 (MQ=255)                                                                                                           ccccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                 <  2:1434237/137‑1 (MQ=255)                                                                                                           ccccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg                 >  1:1434237/1‑137 (MQ=255)                                                                                                             ccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGc                                                                                   >  1:631001/1‑69 (MQ=255)                                                                                                             ccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGc                                                                                   <  2:631001/69‑1 (MQ=255)                                                                                                              cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt            <  2:480617/139‑1 (MQ=255)                                                                                                               aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGt                                                >  1:742236/1‑102 (MQ=255)                                                                                                               aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGt                                                <  2:742236/102‑1 (MQ=255)                                                                                                                   aGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAccc       >  1:1338259/1‑139 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               >  2:1306028/1‑49 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                               <  1:1306028/49‑1 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACg                                                                       >  1:587625/1‑73 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACg                                                                       <  2:587625/73‑1 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACt                                            >  1:1149180/1‑100 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACt                                            <  2:1149180/100‑1 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgag                                      <  2:829813/106‑1 (MQ=255)                                                                                                                     cACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgag                                      >  1:829813/1‑106 (MQ=255)                                                                                                                      aCCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAg    >  2:1449513/1‑139 (MQ=255)                                                                                                                        cTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTa  <  1:1449513/139‑1 (MQ=255)                                                                                                                        cTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTa  >  1:355849/1‑139 (MQ=255)                                                                                                                                  cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCa          <  2:682994/121‑1 (MQ=255)                                                                                                                                  cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCa          >  1:682994/1‑121 (MQ=255)                                                                                                                                        |                                                                                                                           AGAACACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTA  >  NC_000913/177527‑177784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑