Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,034,536 G→A pseudogene (486/663 nt) yedS → pseudogene, outer membrane protein homology, putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,034,5360GA100.0% 199.8 / NA 61pseudogene (486/663 nt)yedSpseudogene, outer membrane protein homology, putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/33);  total (28/33)

GTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACA  >  NC_000913/2034402‑2034671
                                                                                                                                      |                                                                                                                                       
gtCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCg                                                                                                                                     <  2:835880/139‑1 (MQ=255)
           cGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAc                                                                                                                          >  1:683721/1‑139 (MQ=255)
            gTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         <  2:1167745/139‑1 (MQ=255)
              aGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         >  1:175407/1‑137 (MQ=255)
              aGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         <  2:175407/137‑1 (MQ=255)
                aaGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgc                                                                                                                     <  1:654877/139‑1 (MQ=255)
                    aTGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 <  1:1006540/139‑1 (MQ=255)
                    aTGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 <  1:1294468/139‑1 (MQ=255)
                     tGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACtttgctttgc                                                                                                                >  1:666065/1‑139 (MQ=255)
                      ggACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCa                                                                                                               <  2:666065/139‑1 (MQ=255)
                          cGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAatat                                                                                                           <  2:683721/139‑1 (MQ=255)
                            atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttg                                                                                                                      >  2:691216/1‑126 (MQ=255)
                            atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttg                                                                                                                      <  1:691216/126‑1 (MQ=255)
                            atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         <  2:1100556/123‑1 (MQ=255)
                            atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         >  1:1100556/1‑123 (MQ=255)
                            atatGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCa                                                                                                         >  2:706128/1‑139 (MQ=255)
                            atatGCTGCCAGAATTTGGT‑GCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAg                                                                                                        >  2:1240974/1‑139 (MQ=255)
                               tgctgcCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAggg                                                                                                      <  2:1195157/139‑1 (MQ=255)
                                    ccAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGCGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGtaata                                                                                                 >  2:1321006/1‑139 (MQ=255)
                                       gAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGta                                                                                                    <  1:287173/133‑1 (MQ=255)
                                       gAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGta                                                                                                    >  2:287173/1‑133 (MQ=255)
                                              gtggCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATTGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGAt                                                                                                                                   <  2:1067153/95‑1 (MQ=255)
                                              gtggCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGAt                                                                                                                                   >  1:1067153/1‑95 (MQ=255)
                                                ggCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAgtggtg                                                                                     <  1:706128/139‑1 (MQ=255)
                                                  cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 <  2:525235/109‑1 (MQ=255)
                                                  cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 >  1:525235/1‑109 (MQ=255)
                                                  cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         <  1:175217/101‑1 (MQ=255)
                                                  cGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAAct                                                                                                                         >  2:175217/1‑101 (MQ=255)
                                                           tGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATTGTGTTACAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACtttgcttagc                                                                                                                >  1:1009172/1‑101 (MQ=255)
                                                           tGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACtttgctttgc                                                                                                                <  2:1009172/101‑1 (MQ=255)
                                                               ggCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAatat                                                                                                           <  2:1044238/102‑1 (MQ=255)
                                                               ggCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAatat                                                                                                           >  1:1044238/1‑102 (MQ=255)
                                                               ggCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAgg                                                                      <  2:553039/139‑1 (MQ=255)
                                                                  gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTaataat                                                                                               >  1:1066677/1‑111 (MQ=255)
                                                                  gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAg                                                                   >  1:1471536/1‑139 (MQ=255)
                                                                  gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAg                                                                   >  1:1336666/1‑139 (MQ=255)
                                                                  gcTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTCAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTaataat                                                                                               <  2:1066677/111‑1 (MQ=255)
                                                                                  aCGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGa                                                   <  2:1471536/139‑1 (MQ=255)
                                                                                  aCGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGa                                                   <  1:1070311/139‑1 (MQ=255)
                                                                                     gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgc                                                                                                                     >  1:956107/1‑70 (MQ=255)
                                                                                     gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgc                                                                                                                     <  2:956107/70‑1 (MQ=255)
                                                                                     gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGt                                                                 <  2:527437/122‑1 (MQ=255)
                                                                                     gTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGt                                                                 >  1:527437/1‑122 (MQ=255)
                                                                                          gCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 >  2:965238/1‑69 (MQ=255)
                                                                                          gCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 <  1:965238/69‑1 (MQ=255)
                                                                                                    ttGCAACCTACCGTAACAATTGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCaa                                 <  2:1156251/139‑1 (MQ=255)
                                                                                                                gTAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGa                     <  1:1240974/139‑1 (MQ=255)
                                                                                                                 tAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 >  2:454287/1‑46 (MQ=255)
                                                                                                                 tAACAATGGCTTCTTTGGTTAAGTCGATGGCTTGAActttgctttg                                                                                                                 <  1:454287/46‑1 (MQ=255)
                                                                                                                   aCAATGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATgg                  >  1:730014/1‑139 (MQ=255)
                                                                                                                      aTGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAgtggtg                                                                                     <  1:329919/69‑1 (MQ=255)
                                                                                                                      aTGGCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAAGATCAGGGTAATAATGAAAgtggtg                                                                                     >  2:329919/1‑69 (MQ=255)
                                                                                                                       tGGCTTCTTTGGTTAAGTCGATGGCTTGAACtttgctttgc                                                                                                                >  1:1217043/1‑41 (MQ=255)
                                                                                                                       tGGCTTCTTTGGTTAAGTCGATGGCTTGAACtttgctttgc                                                                                                                <  2:1217043/41‑1 (MQ=255)
                                                                                                                        ggCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGa                                                                                             <  2:367960/59‑1 (MQ=255)
                                                                                                                        ggCTTCTTTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGa                                                                                             >  1:367960/1‑59 (MQ=255)
                                                                                                                             cttTGGTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATg        >  1:1482859/1‑139 (MQ=255)
                                                                                                                                 ggTTAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCaa    <  1:1321006/139‑1 (MQ=255)
                                                                                                                                   ttAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATGATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACa  >  1:871938/1‑139 (MQ=255)
                                                                                                                                   ttAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCggg                                                                          <  2:149768/67‑1 (MQ=255)
                                                                                                                                   ttAAGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCggg                                                                          >  1:149768/1‑67 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GTCGTATATGACGTAGAAGCATGGACCGATATGCTGCCAGAATTTGGTGGCGATACCTATGCTGGCGCTGACAACTTCATGAACGGTCGTGCTAATGGTGTTGCAACCTACCGTAACAATGGCTTCTTTGGTTAGGTCGATGGCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACA  >  NC_000913/2034402‑2034671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑