Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	194.1 / NA	60	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/32);  total (28/32)

GCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGA  >  NC_000913/2049205‑2049469                                                                                                                                         |                                                                                                                                      gCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACGTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGAt                                                                                                                                >  2:914913/1‑139 (MQ=255)     tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                            <  1:1378349/139‑1 (MQ=255)       ccGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          <  2:1287669/139‑1 (MQ=255)       ccGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          <  1:372717/139‑1 (MQ=255)           tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGAc                                                                                                                         <  1:116228/136‑1 (MQ=255)           tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                                                      >  1:1400365/1‑139 (MQ=255)           tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACATATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGAc                                                                                                                         >  2:116228/1‑136 (MQ=255)            aCAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATa                                                                                                                     <  1:89709/139‑1 (MQ=255)                     ggggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTg                                                                                                                              <  2:259631/121‑1 (MQ=255)                     ggggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTg                                                                                                                              >  1:259631/1‑121 (MQ=255)                             tCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTa                                                                                                    >  1:1349751/1‑139 (MQ=255)                                     cGGCAACGCTCGAAAATGGCGATTAGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CAGAACGTCGCGAATGCTGAAATCACACTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACCACACTAACAGCAAc                                                                                            >  1:921777/1‑139 (MQ=255)                                      ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           <  1:442103/139‑1 (MQ=255)                                      ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           <  1:242993/139‑1 (MQ=255)                                      ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTCACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGCCACTAACAGCAACa                                                                                           <  1:914913/139‑1 (MQ=255)                                         aaCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCaa                                                                                             >  1:844091/1‑139 (MQ=255)                                         aaCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTc                                                                                        >  1:1406021/1‑139 (MQ=255)                                           cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                            >  1:1022547/1‑101 (MQ=255)                                           cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                            <  2:1022547/101‑1 (MQ=255)                                                gAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATa                                                                                 >  1:1351916/1‑139 (MQ=255)                                                  aaaTGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:1400365/139‑1 (MQ=255)                                                  aaaTGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:717146/139‑1 (MQ=255)                                                       gCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:844091/139‑1 (MQ=255)                                                          aTTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCaa                                                                       >  2:455567/1‑139 (MQ=255)                                                              gATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                                   <  1:307800/139‑1 (MQ=255)                                                              gATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                                   <  2:1351916/139‑1 (MQ=255)                                                                tGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGAt                                                                 >  2:1153480/1‑139 (MQ=255)                                                                        acaGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         <  1:1153480/139‑1 (MQ=255)                                                                             gACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              <  2:1456716/129‑1 (MQ=255)                                                                             gACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              >  1:1456716/1‑129 (MQ=255)                                                                              aCCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACaga                                                                             <  2:945116/113‑1 (MQ=255)                                                                              aCCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACaga                                                                             >  1:945116/1‑113 (MQ=255)                                                                               ccTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                                  <  1:455567/139‑1 (MQ=255)                                                                                   tgtg‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          <  1:742271/63‑1 (MQ=255)                                                                                   tgtg‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                          >  2:742271/1‑63 (MQ=255)                                                                                   tgtg‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTCCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACa                                              >  1:1262751/1‑139 (MQ=255)                                                                                      g‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCAc                                                                                                           >  2:543038/1‑75 (MQ=255)                                                                                      g‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCAc                                                                                                           <  1:543038/75‑1 (MQ=255)                                                                                      g‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACAttt                                           >  1:1369478/1‑139 (MQ=255)                                                                                            ccGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTa                                          <  1:54336/139‑1 (MQ=255)                                                                                               aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         >  1:579413/1‑121 (MQ=255)                                                                                               aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                         <  2:579413/121‑1 (MQ=255)                                                                                               aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                                        <  1:1211985/138‑1 (MQ=255)                                                                                               aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                                        >  2:1211985/1‑138 (MQ=255)                                                                                                                     cGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGac                                                                                                        >  2:1468258/1‑52 (MQ=255)                                                                                                                     cGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGac                                                                                                        <  1:1468258/52‑1 (MQ=255)                                                                                                                      gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               <  2:328176/76‑1 (MQ=255)                                                                                                                      gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACa                                                                               >  1:328176/1‑76 (MQ=255)                                                                                                                      gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAAc                >  1:530166/1‑139 (MQ=255)                                                                                                                         ggCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                                  <  2:1232627/102‑1 (MQ=255)                                                                                                                         ggCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                                  >  1:1232627/1‑102 (MQ=255)                                                                                                                          gCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              >  2:1222576/1‑89 (MQ=255)                                                                                                                          gCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              <  1:1222576/89‑1 (MQ=255)                                                                                                                              ccTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                                   <  2:1444635/80‑1 (MQ=255)                                                                                                                              ccTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                                   >  1:1444635/1‑80 (MQ=255)                                                                                                                                tCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              <  2:565545/83‑1 (MQ=255)                                                                                                                                tCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                                                              >  1:565545/1‑83 (MQ=255)                                                                                                                                  gAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           <  2:18641/52‑1 (MQ=255)                                                                                                                                  gAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                           >  1:18641/1‑52 (MQ=255)                                                                                                                                    aGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGa  <  2:1369478/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTG‑‑‑‑‑CCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGCTGAGCGA  >  NC_000913/2049205‑2049469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑