Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,257	C→T	*271* (TAG→TAA)	yaeI ←	phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,257	0	C	T	100.0%	201.4 / NA	63	*271* (TAG→TAA)	yaeI	phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAC  >  NC_000913/184124‑184391                                                                                                                                      |                                                                                                                                       tgtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   <  2:1281933/139‑1 (MQ=255)  gtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTaca                                                                                                                                    >  2:1444122/1‑137 (MQ=255)  gtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTaca                                                                                                                                    <  1:1444122/137‑1 (MQ=255)      ttATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAAGCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGtt                                                                                                                              >  1:996709/1‑139 (MQ=255)                    ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACt                                                                                                                >  2:779632/1‑139 (MQ=255)                        tggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCg                                                                                                            <  1:244293/139‑1 (MQ=255)                          gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                  <  2:781718/115‑1 (MQ=255)                          gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                  >  1:781718/1‑115 (MQ=255)                          gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCggg                                                                                                          >  1:993948/1‑139 (MQ=255)                               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   <  2:303185/109‑1 (MQ=255)                               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTacac                                                                                                                                   >  1:303185/1‑109 (MQ=255)                                    cTCGACTGTGATCTCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCCGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCa                                                                                                <  2:996709/139‑1 (MQ=255)                                       gACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                             <  1:939742/139‑1 (MQ=255)                                                   aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa                                                                                 >  1:1300394/1‑139 (MQ=255)                                                      aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAAc                                                                              >  2:622551/1‑139 (MQ=255)                                                        gCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTg                                                                            >  1:393609/1‑139 (MQ=255)                                                        gCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTg                                                                            >  2:487296/1‑139 (MQ=255)                                                          cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         <  1:434156/124‑1 (MQ=255)                                                          cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         >  2:434156/1‑124 (MQ=255)                                                           tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                         <  2:993948/139‑1 (MQ=255)                                                           tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGACGGCAGTTCAGACGc                                                                                           <  2:1007553/121‑1 (MQ=255)                                                           tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGAGTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGc                                                                                           >  1:1007553/1‑121 (MQ=255)                                                                cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                    <  2:1300394/139‑1 (MQ=255)                                                                     aGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                               <  1:779632/139‑1 (MQ=255)                                                                       aCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGtt                                                             <  1:487296/139‑1 (MQ=255)                                                                                   gCGTAAAACTCATAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTAc                                                                                                                 >  2:460294/1‑75 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTAc                                                                                                                 <  1:460294/75‑1 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa                                                                                   <  2:484851/105‑1 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa                                                                                   >  1:484851/1‑105 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                   >  2:795132/1‑121 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                   <  1:795132/121‑1 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt                                                 >  2:873350/1‑139 (MQ=255)                                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt                                                 >  2:1071422/1‑139 (MQ=255)                                                                                               aaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAg                                     <  2:1057182/139‑1 (MQ=255)                                                                                                            tttATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCgg                        >  2:987675/1‑139 (MQ=255)                                                                                                            tttATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCgg                        <  2:393609/139‑1 (MQ=255)                                                                                                                 tCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                          <  2:228517/68‑1 (MQ=255)                                                                                                                 tCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                          >  1:228517/1‑68 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACGCCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCtt                                              <  1:506569/108‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       <  2:197303/51‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                       >  1:197303/1‑51 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                             >  1:1342074/1‑61 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                             <  2:1342074/61‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                   <  2:967840/87‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                   >  1:967840/1‑87 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCtt                                              >  2:506569/1‑108 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                      <  1:602115/116‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                      >  2:602115/1‑116 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCa                                  <  1:570590/120‑1 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCa                                  >  2:570590/1‑120 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc               >  1:883823/1‑139 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc               >  1:565490/1‑139 (MQ=255)                                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc               >  1:1033507/1‑139 (MQ=255)                                                                                                                       gATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt             <  1:873350/139‑1 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         >  1:287718/1‑60 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                         <  2:287718/60‑1 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                         <  1:155427/76‑1 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                         >  2:155427/1‑76 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt             >  1:1223075/1‑136 (MQ=255)                                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt             <  2:1223075/136‑1 (MQ=255)                                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                   >  1:162707/1‑125 (MQ=255)                                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                   <  2:162707/125‑1 (MQ=255)                                                                                                                                  cGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAc  <  1:987675/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAC  >  NC_000913/184124‑184391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑