Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,233,597 | G→A | *240* (TAG→TAA) | sanA → | DUF218 superfamily vancomycin high temperature exclusion protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,233,597 | 0 | G | A | 100.0% | 133.3 / NA | 42 | *240* (TAG→TAA) | sanA | DUF218 superfamily vancomycin high temperature exclusion protein |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/23); total (19/23) |
TTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAA > NC_000913/2233464‑2233712 | ttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATg > 1:279910/1‑139 (MQ=255) tAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATgg < 2:1333163/139‑1 (MQ=255) aaCCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag < 1:1329375/139‑1 (MQ=255) gCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTg < 2:393163/139‑1 (MQ=255) gCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCg < 2:724800/139‑1 (MQ=255) gTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt < 2:279910/139‑1 (MQ=255) tATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTcc < 2:658859/139‑1 (MQ=255) ggTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGtt > 2:1504482/1‑139 (MQ=255) ggAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgcg > 1:1173637/1‑139 (MQ=255) ggAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgcg < 1:871039/139‑1 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag > 2:1369791/1‑81 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag < 1:1369791/81‑1 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt > 2:803782/1‑104 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt < 1:803782/104‑1 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc < 2:273733/114‑1 (MQ=255) tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc > 1:273733/1‑114 (MQ=255) tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGTATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc < 1:625003/120‑1 (MQ=255) tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc > 2:625003/1‑120 (MQ=255) cgccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgcgcg > 2:536459/1‑139 (MQ=255) ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt > 2:318958/1‑87 (MQ=255) ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt < 1:318958/87‑1 (MQ=255) ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc < 2:943425/115‑1 (MQ=255) ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc > 1:943425/1‑115 (MQ=255) acacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcgc > 2:1100002/1‑139 (MQ=255) acCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGa > 2:394298/1‑139 (MQ=255) ccGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGAtt > 1:1488554/1‑139 (MQ=255) aCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAgg < 1:840510/128‑1 (MQ=255) aCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAgg > 2:840510/1‑128 (MQ=255) cAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGa < 2:1393937/111‑1 (MQ=255) cAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGa > 1:1393937/1‑111 (MQ=255) aGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa < 1:627524/139‑1 (MQ=255) aGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa < 2:1488554/139‑1 (MQ=255) aGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa < 1:1504482/139‑1 (MQ=255) tACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACgg < 1:394298/139‑1 (MQ=255) aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACggg > 2:1111616/1‑139 (MQ=255) aTTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATcc < 2:1410865/73‑1 (MQ=255) aTTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATcc > 1:1410865/1‑73 (MQ=255) ttACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTaaaaa < 1:1100002/139‑1 (MQ=255) gAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcgc < 2:1123854/110‑1 (MQ=255) gAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcgc > 1:1123854/1‑110 (MQ=255) aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGttttt > 1:839502/1‑51 (MQ=255) aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGttttt < 2:839502/51‑1 (MQ=255) agtaatTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGaa > 2:448206/1‑81 (MQ=255) agtaatTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGaa < 1:448206/81‑1 (MQ=255) | TTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAA > NC_000913/2233464‑2233712 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |