Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,478	T→C	V6V (GTA→GTG)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,478	0	T	C	100.0%	235.5 / NA	64	V6V (GTA→GTG)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/35);  total (29/35)

TCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTT  >  NC_000913/2263356‑2263611                                                                                                                           |                                                                                                                                      tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                       <  1:889110/139‑1 (MQ=255) tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                       <  2:1201747/139‑1 (MQ=255)         cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                               <  1:297496/139‑1 (MQ=255)               gCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                    >  2:983869/1‑128 (MQ=255)               gCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                    <  1:983869/128‑1 (MQ=255)                 cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       >  2:542300/1‑139 (MQ=255)                   ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                                     >  2:1269022/1‑139 (MQ=255)                   ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                                     <  2:1443557/139‑1 (MQ=255)                          aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa                                                                                              >  1:142327/1‑139 (MQ=255)                          aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa                                                                                              >  2:324944/1‑139 (MQ=255)                           gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             >  2:758552/1‑139 (MQ=255)                           gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  1:54355/139‑1 (MQ=255)                           gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  1:324944/139‑1 (MQ=255)                                cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAt                                                                                        >  2:292673/1‑139 (MQ=255)                                         tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                                 <  2:1347538/89‑1 (MQ=255)                                         tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                                 >  1:1347538/1‑89 (MQ=255)                                         tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCa                                                                                     >  2:726210/1‑133 (MQ=255)                                         tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCa                                                                                     <  1:726210/133‑1 (MQ=255)                                           ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                                 <  2:1212164/87‑1 (MQ=255)                                           ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                                 >  1:1212164/1‑87 (MQ=255)                                           ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGa                                                                                         <  2:1300638/127‑1 (MQ=255)                                           ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGa                                                                                         >  1:1300638/1‑127 (MQ=255)                                                cTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaa                                                                        >  1:21771/1‑139 (MQ=255)                                                cTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaa                                                                        >  1:1423811/1‑139 (MQ=255)                                                  gCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaa                                                                      >  1:155151/1‑139 (MQ=255)                                                   cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTg                                                                                                                            >  2:1411194/1‑84 (MQ=255)                                                   cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTg                                                                                                                            <  1:1411194/84‑1 (MQ=255)                                                   cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                                     >  2:986838/1‑139 (MQ=255)                                                        gcgcCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                   <  1:1407199/120‑1 (MQ=255)                                                        gcgcCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                   >  2:1407199/1‑120 (MQ=255)                                                         cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcgc                                                               >  2:166556/1‑139 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAAc                                                                                                                              >  2:53982/1‑72 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAAc                                                                                                                              <  1:53982/72‑1 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                                           <  2:155151/139‑1 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                                           <  1:542300/139‑1 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                                           <  2:1423811/139‑1 (MQ=255)                                                             gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                                           <  2:142327/139‑1 (MQ=255)                                                              cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAcc                                                          <  1:166556/139‑1 (MQ=255)                                                                          gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  1:758552/139‑1 (MQ=255)                                                                          gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  2:1275316/139‑1 (MQ=255)                                                                             tCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             >  1:47323/1‑89 (MQ=255)                                                                             tCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  2:47323/89‑1 (MQ=255)                                                                                  tcttctTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACaa                                      >  2:427754/1‑139 (MQ=255)                                                                                     tcttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGAt                                   <  1:747395/139‑1 (MQ=255)                                                                                      cttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATc                                  <  2:151567/139‑1 (MQ=255)                                                                                         tGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       >  1:974944/1‑67 (MQ=255)                                                                                         tGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       <  2:974944/67‑1 (MQ=255)                                                                                             tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                    >  1:398742/1‑50 (MQ=255)                                                                                             tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                    <  2:398742/50‑1 (MQ=255)                                                                                              ccGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       <  2:1239607/62‑1 (MQ=255)                                                                                              ccGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       >  1:1239607/1‑62 (MQ=255)                                                                                              ccGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTACTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGACTTAGGGTTAAAGACAAGATCGCGCTGaa                          >  2:973319/1‑139 (MQ=255)                                                                                               cGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtc                                                                                                                 <  2:1460532/51‑1 (MQ=255)                                                                                               cGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtc                                                                                                                 >  1:1460532/1‑51 (MQ=255)                                                                                               cGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGaaa                         <  1:986838/139‑1 (MQ=255)                                                                                                ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                   >  2:349081/1‑80 (MQ=255)                                                                                                ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                   <  1:349081/80‑1 (MQ=255)                                                                                                ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTa                                                   <  2:785630/112‑1 (MQ=255)                                                                                                ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTa                                                   >  1:785630/1‑112 (MQ=255)                                                                                                    ttttCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGttt                    <  1:427754/139‑1 (MQ=255)                                                                                                         gCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:292673/139‑1 (MQ=255)                                                                                                            cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg            >  1:124260/1‑139 (MQ=255)                                                                                                                     gTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACt   <  1:1269022/139‑1 (MQ=255)                                                                                                                      tCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACtt  <  2:21771/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                      TCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTT  >  NC_000913/2263356‑2263611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑