Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,274,166 A→G K601K (AAA→AAG)  yejA → microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,1660AG100.0% 200.1 / NA 55K601K (AAA→AAG) yejAmicrocin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

CGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCG  >  NC_000913/2274042‑2274280
                                                                                                                            |                                                                                                                  
cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                              >  1:1115732/1‑131 (MQ=255)
cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                              <  2:1115732/131‑1 (MQ=255)
          tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                            >  2:1179995/1‑139 (MQ=255)
          tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                            >  1:501442/1‑139 (MQ=255)
                        aTAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCt                                                                              <  1:1179995/139‑1 (MQ=255)
                               ctcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                       <  1:867917/139‑1 (MQ=255)
                                  ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCCGATTCGCCGTCTGTTGCTGGTg                                                                    >  2:765893/1‑139 (MQ=255)
                                          ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                       <  2:1195850/128‑1 (MQ=255)
                                          ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                       >  1:1195850/1‑128 (MQ=255)
                                                       cTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGAtt                                               >  1:980835/1‑139 (MQ=255)
                                                         ataGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                             <  2:501442/139‑1 (MQ=255)
                                                                cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGcc                                                                                  >  1:1253145/1‑95 (MQ=255)
                                                                cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGcc                                                                                  <  2:1253145/95‑1 (MQ=255)
                                                                cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                      >  2:228848/1‑139 (MQ=255)
                                                                cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                      <  2:168709/139‑1 (MQ=255)
                                                                cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                      >  2:1311423/1‑139 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                              <  2:1450425/66‑1 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                              >  1:1450425/1‑66 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgag                                                    >  2:383926/1‑122 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgag                                                    <  1:383926/124‑3 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCAcc                                         >  1:998335/1‑135 (MQ=255)
                                                                 ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCAcc                                         <  2:998335/135‑1 (MQ=255)
                                                                           ccTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCAt                           <  2:1208351/139‑1 (MQ=255)
                                                                             tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCg                         >  1:634452/1‑139 (MQ=255)
                                                                              ggtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                 >  1:1376181/1‑82 (MQ=255)
                                                                              ggtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                 <  2:1376181/82‑1 (MQ=255)
                                                                              ggtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGt                        <  1:228848/139‑1 (MQ=255)
                                                                               gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     >  1:1179230/1‑93 (MQ=255)
                                                                               gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     <  2:1179230/93‑1 (MQ=255)
                                                                                 ggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                                        <  1:1130869/104‑1 (MQ=255)
                                                                                 ggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                                        >  2:1130869/1‑104 (MQ=255)
                                                                                  gtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                    <  1:1439679/107‑1 (MQ=255)
                                                                                  gtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                    >  2:1439679/1‑107 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGgaga                                                                                                            <  2:332076/50‑1 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGgaga                                                                                                            >  1:332076/1‑50 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     >  1:805985/1‑89 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     <  2:805985/89‑1 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                   >  1:792386/1‑91 (MQ=255)
                                                                                   tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                   <  2:792386/91‑1 (MQ=255)
                                                                                    aTGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAAt                  >  2:780751/1‑139 (MQ=255)
                                                                                     tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAAtt                 >  1:1047615/1‑139 (MQ=255)
                                                                                          tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                       >  1:1350407/1‑96 (MQ=255)
                                                                                          tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                       <  2:1350407/96‑1 (MQ=255)
                                                                                           cAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCt           <  1:780751/139‑1 (MQ=255)
                                                                                             aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTgg         >  1:724364/1‑139 (MQ=255)
                                                                                                  gCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtc    <  2:724364/139‑1 (MQ=255)
                                                                                                  gCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtc    >  2:1084072/1‑139 (MQ=255)
                                                                                                   cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     <  2:398829/73‑1 (MQ=255)
                                                                                                   cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                     >  1:398829/1‑73 (MQ=255)
                                                                                                   cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACtttt                               <  1:1301974/111‑1 (MQ=255)
                                                                                                   cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACtttt                               >  2:1301974/1‑111 (MQ=255)
                                                                                                      ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                    >  2:599269/1‑55 (MQ=255)
                                                                                                      ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                    <  1:599269/55‑1 (MQ=255)
                                                                                                                   cGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCAATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccg  >  2:722387/1‑124 (MQ=255)
                                                                                                                   cGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCAATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccg  <  1:722387/124‑1 (MQ=255)
                                                                                                                            |                                                                                                                  
CGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCG  >  NC_000913/2274042‑2274280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑