Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,574	T→C	S343S (AGT→AGC)	rseP →	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,574	0	T	C	100.0%	181.7 / NA	50	S343S (AGT→AGC)	rseP	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

CGATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGT  >  NC_000913/197438‑197708                                                                                                                                         |                                                                                                                                       cGATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCAt                                                                                                                                      <  1:164532/139‑1 (MQ=255)   aTTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                    >  1:410210/1‑139 (MQ=255)   aTTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                    >  1:603957/1‑139 (MQ=255)           tGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaaa                                                                                                                            <  2:603957/139‑1 (MQ=255)                           aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                            >  1:758331/1‑139 (MQ=255)                                  ttCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACt                                                                                                     >  2:128662/1‑139 (MQ=255)                                         gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGagcaac                                                                                              >  2:690904/1‑139 (MQ=255)                                         gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              <  1:128662/139‑1 (MQ=255)                                         gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              >  2:546269/1‑139 (MQ=255)                                                    ataAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGcc                                                                                   >  1:1395882/1‑139 (MQ=255)                                                        aGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                     >  1:1253496/1‑133 (MQ=255)                                                        aGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                                     <  2:1253496/133‑1 (MQ=255)                                                           tGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                            <  2:410210/139‑1 (MQ=255)                                                            gTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGt                                                                                        >  2:988786/1‑126 (MQ=255)                                                            gTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGt                                                                                        <  1:988786/126‑1 (MQ=255)                                                             tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGa                                                                                                         >  2:794833/1‑108 (MQ=255)                                                             tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGa                                                                                                         <  1:794833/108‑1 (MQ=255)                                                             tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                          >  1:1261806/1‑139 (MQ=255)                                                               cGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCg                                                                        <  1:462363/139‑1 (MQ=255)                                                                            cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              <  1:164843/104‑1 (MQ=255)                                                                            cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              >  2:164843/1‑104 (MQ=255)                                                                              ttCAACGCCATCGTCGAAGTCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTg                                                                                                                         <  1:1006918/75‑1 (MQ=255)                                                                              ttCAACGCCATCGTCGAAGTCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTg                                                                                                                         >  2:1006918/1‑75 (MQ=255)                                                                               tCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGAt                                                        >  2:249434/1‑139 (MQ=255)                                                                               tCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGAt                                                        >  1:507603/1‑139 (MQ=255)                                                                                 aaCGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGa                                                      <  1:249434/139‑1 (MQ=255)                                                                                   cGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACa                                                    >  1:573810/1‑139 (MQ=255)                                                                                               aGCCACGGAC‑AAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  1:1110901/139‑1 (MQ=255)                                                                                                gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAGCAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  1:690904/139‑1 (MQ=255)                                                                                                gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  2:758331/139‑1 (MQ=255)                                                                                                gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  2:507603/139‑1 (MQ=255)                                                                                                gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  1:546269/139‑1 (MQ=255)                                                                                                 ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                      <  2:1261806/139‑1 (MQ=255)                                                                                                    cGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                            >  2:1163038/1‑98 (MQ=255)                                                                                                    cGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                            <  1:1163038/98‑1 (MQ=255)                                                                                                     ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCa                                                                     <  2:464121/104‑1 (MQ=255)                                                                                                     ggACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCa                                                                     >  1:464121/1‑104 (MQ=255)                                                                                                           aaCGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                  <  1:765428/69‑1 (MQ=255)                                                                                                           aaCGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                                  >  2:765428/1‑69 (MQ=255)                                                                                                                ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                 >  1:83852/1‑49 (MQ=255)                                                                                                                ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                 <  2:83852/49‑1 (MQ=255)                                                                                                                ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGcc                       >  2:315768/1‑139 (MQ=255)                                                                                                                       gatgaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCtt                >  1:410614/1‑139 (MQ=255)                                                                                                                          gaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              <  2:660228/58‑1 (MQ=255)                                                                                                                          gaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              >  1:660228/1‑58 (MQ=255)                                                                                                                           aaGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                 >  2:606655/1‑38 (MQ=255)                                                                                                                           aaGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                                 <  1:606655/38‑1 (MQ=38)                                                                                                                                 aCGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCttatta      >  1:678036/1‑139 (MQ=255)                                                                                                                                   ggTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGc    <  2:410614/139‑1 (MQ=255)                                                                                                                                     tCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGt  <  2:678036/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGT  >  NC_000913/197438‑197708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑