Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,381,563	T→C	intergenic (‑20/‑45)	elaA ← / → rbn	GNAT family putative N‑acetyltransferase/RNase BN, tRNA processing enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,381,563	0	T	C	100.0%	122.4 / NA	35	intergenic (‑20/‑45)	elaA/rbn	GNAT family putative N‑acetyltransferase/RNase BN, tRNA processing enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

ATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGAC  >  NC_000913/2381430‑2381696                                                                                                                                      |                                                                                                                                      atCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGcc                                                                                                                                  <  2:658591/139‑1 (MQ=255)            ggCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGcc                                                                                                                       >  1:789970/1‑139 (MQ=255)                 ttCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAat                                                                                                                  >  2:1071100/1‑139 (MQ=255)                                aacaCCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGtg                                                                                                   <  2:789970/139‑1 (MQ=255)                                            cGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATgg                                                                                       >  1:195984/1‑139 (MQ=255)                                                      ataaGGCATAAAGCTGGGAAACCGGTAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAAtttt                                                                             >  1:1067321/1‑139 (MQ=255)                                                      ataaGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGaa                                                                                                        <  2:1040954/112‑1 (MQ=255)                                                      ataaGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGaa                                                                                                        >  1:1040954/1‑112 (MQ=255)                                                           gCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAg                                                                        <  2:195984/139‑1 (MQ=255)                                                              tAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGACTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTa                                                                     >  2:1081796/1‑139 (MQ=255)                                                                    tGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAg                                                               <  1:601314/139‑1 (MQ=255)                                                                                       agTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGaa                                                                                                        <  2:285849/79‑1 (MQ=255)                                                                                       agTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGaa                                                                                                        >  1:285849/1‑79 (MQ=255)                                                                                           aTGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATAtttttt                                                                                                           >  1:744226/1‑72 (MQ=255)                                                                                           aTGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATAtttttt                                                                                                           <  2:744226/72‑1 (MQ=255)                                                                                           aTGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACgcgc                                        >  1:1194526/1‑139 (MQ=255)                                                                                            tGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGcc                                                                                                                                  <  1:750881/48‑1 (MQ=255)                                                                                            tGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGcc                                                                                                                                  >  2:1466500/1‑48 (MQ=255)                                                                                            tGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGcc                                                                                                                                  >  2:750881/1‑48 (MQ=255)                                                                                            tGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGcc                                                                                                                                  <  1:1466500/48‑1 (MQ=255)                                                                                                gATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGt                                   <  2:1067321/139‑1 (MQ=255)                                                                                                  tCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCa                                 <  1:1071100/139‑1 (MQ=255)                                                                                                   cTTGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCAc                                >  2:1377790/1‑139 (MQ=255)                                                                                                     tGCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACgg                              >  2:1441624/1‑139 (MQ=255)                                                                                                      gCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGcc                                                     >  1:1017929/1‑115 (MQ=255)                                                                                                      gCCATTCAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGcc                                                     <  2:1017929/115‑1 (MQ=255)                                                                                                            cAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTa                                                                         >  2:787902/1‑89 (MQ=255)                                                                                                            cAATCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTa                                                                         <  1:787902/89‑1 (MQ=255)                                                                                                             aaTCATTATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAAttttt                                                                            <  1:552195/85‑1 (MQ=255)                                                                                                             aaTCATAATGCTCTCCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAAttttt                                                                            >  2:552195/1‑85 (MQ=255)                                                                                                                        tctcCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGt                                   >  1:1215515/1‑115 (MQ=255)                                                                                                                        tctcCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGt                                   <  2:1215515/115‑1 (MQ=255)                                                                                                                        tctcCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCa           <  2:1194526/139‑1 (MQ=255)                                                                                                                         ctcCTTTTAGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCaa          >  2:1069920/1‑139 (MQ=255)                                                                                                                                 aGATGCATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGAc  <  1:1081796/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGATGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGAC  >  NC_000913/2381430‑2381696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑