Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	220,239	C→T	L230L (CTG→CTA)	metQ ←	DL‑methionine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	220,239	0	C	T	100.0%	152.8 / NA	48	L230L (CTG→CTA)	metQ	DL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/30);  total (18/30)

CCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGC  >  NC_000913/220105‑220365                                                                                                                                       |                                                                                                                               ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGt                                                                                                                            <  2:893353/139‑1 (MQ=255) ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGt                                                                                                                            <  1:675550/139‑1 (MQ=255) ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGt                                                                                                                            <  1:285024/139‑1 (MQ=255)   tAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGttt                                                                                                                          <  2:1388014/139‑1 (MQ=255)    aaaaaTTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTta                                                                                                                         <  1:946568/139‑1 (MQ=255)          tACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgtacg                                                                                                                   <  1:548657/139‑1 (MQ=255)            ccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                 <  1:678301/139‑1 (MQ=255)            ccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                 <  2:1324179/139‑1 (MQ=255)             cAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACgggg                                                                                                                <  2:49428/139‑1 (MQ=255)              aGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACTTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGa                                                                                                               <  2:1058431/139‑1 (MQ=255)                  tttAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGActct                                                                                                           <  2:96927/139‑1 (MQ=255)                    tAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCttt                                                                                                         <  2:907065/139‑1 (MQ=255)                        aGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCt                                                                                                     <  2:1341517/139‑1 (MQ=255)                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCt                                                                                                     <  2:916574/137‑1 (MQ=255)                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCt                                                                                                     >  1:916574/1‑137 (MQ=255)                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                   <  2:579939/139‑1 (MQ=255)                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                   >  1:834268/1‑139 (MQ=255)                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                   >  1:579939/1‑139 (MQ=255)                                                  ctgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCt                                                                                   <  1:685323/131‑1 (MQ=255)                                                  ctgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCt                                                                                   >  2:685323/1‑131 (MQ=255)                                                         gTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg                                                                    <  1:84559/139‑1 (MQ=255)                                                                   tCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggc                                                          >  2:710958/1‑139 (MQ=255)                                                                       aCTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                      >  2:1399304/1‑139 (MQ=255)                                                                        cTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa                                                     >  1:836572/1‑139 (MQ=255)                                                                                    gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                           >  2:156068/1‑121 (MQ=255)                                                                                    gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                           <  1:156068/121‑1 (MQ=255)                                                                                         aTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg                                                                    <  2:972877/107‑1 (MQ=255)                                                                                         aTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg                                                                    >  1:972877/1‑107 (MQ=255)                                                                                              ttcACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCa                               >  1:1385949/1‑139 (MQ=255)                                                                                                        cGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                      >  1:150915/1‑106 (MQ=255)                                                                                                        cGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                      <  2:150915/106‑1 (MQ=255)                                                                                                            gTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                 <  2:834268/139‑1 (MQ=255)                                                                                                            gTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                 <  1:1399304/139‑1 (MQ=255)                                                                                                               tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt                                                >  2:1102923/1‑105 (MQ=255)                                                                                                               tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt                                                <  1:1102923/105‑1 (MQ=255)                                                                                                               tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                 <  1:92/104‑1 (MQ=255)                                                                                                               tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                 >  2:92/1‑104 (MQ=255)                                                                                                                 tGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCgtcgtc            <  1:710958/139‑1 (MQ=255)                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGaa     >  1:1394992/1‑139 (MQ=255)                                                                                                                         tCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                         >  2:43227/1‑70 (MQ=255)                                                                                                                         tCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg                                                                         <  1:43227/70‑1 (MQ=255)                                                                                                                          cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg                                  >  1:855791/1‑108 (MQ=255)                                                                                                                          cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg                                  <  2:855791/108‑1 (MQ=255)                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgcgc                  >  1:108536/1‑123 (MQ=255)                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgcgc                  <  2:108536/123‑1 (MQ=255)                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAAcgc  <  2:1394992/139‑1 (MQ=255)                                                                                                                             gcgTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                       <  1:1378507/100‑1 (MQ=255)                                                                                                                             gcgTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                       >  2:1378507/1‑100 (MQ=255)                                                                                                                                       |                                                                                                                               CCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGC  >  NC_000913/220105‑220365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑