Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,552,094	G→A	I15I (ATC→ATT)	ypeA ←	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,552,094	0	G	A	100.0%	180.6 / NA	56	I15I (ATC→ATT)	ypeA	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/28);  total (28/28)

CCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAA  >  NC_000913/2551961‑2552226                                                                                                                                      |                                                                                                                                       ccATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAc                                                                                                                                   <  2:1416020/139‑1 (MQ=255)  cATCTCCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                                                                  <  2:1091870/139‑1 (MQ=255)  cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                                                                  >  1:223485/1‑139 (MQ=255)           tCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGCCGTCTTGTTTCTTCTTACCCTCGATGTCCATTTCCGGATGGTTCACGGGACCCAGCAGGTGGCAACGCCCCCAAAGGGAAATGCCTTCTTCaaaa                                                                                                                         >  1:642770/1‑139 (MQ=255)            ccGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAAt                                                                                                                        <  2:858064/139‑1 (MQ=255)                    gTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg                                                                                                                <  1:775452/139‑1 (MQ=255)                      cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg                                                                                                              >  2:84891/1‑139 (MQ=255)                      cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg                                                                                                              >  2:466634/1‑139 (MQ=255)                         cGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCGCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaaa                                                                                                           <  2:804065/139‑1 (MQ=255)                                   aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtc                                                                                                 >  1:751329/1‑139 (MQ=255)                                     cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtct                                                                                                <  1:638085/138‑1 (MQ=255)                                     cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtct                                                                                                >  2:638085/1‑138 (MQ=255)                                       aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa                                                                                            <  1:466634/139‑1 (MQ=255)                                                  acTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGtt                                                                                >  1:897269/1‑139 (MQ=255)                                                             ggTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg                                                                     <  2:897269/139‑1 (MQ=255)                                                                 cATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCATATCT‑CCAT‑GGTATTCGTTACCTTATTGCGGGtt                                                                 >  1:965971/1‑139 (MQ=255)                                                                        cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGc                                                          >  2:1370105/1‑139 (MQ=255)                                                                        cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGc                                                          <  2:500744/139‑1 (MQ=255)                                                                            cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAt                                                      <  2:965971/139‑1 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaaa                                                                                                                         <  1:1340153/72‑1 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaaa                                                                                                                         >  2:1340153/1‑72 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa                                                                                                         <  2:1245730/88‑1 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt                                                                                                  <  1:583061/95‑1 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt                                                                                                  >  2:583061/1‑95 (MQ=255)                                                                              aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAACCTTCCTGGCGAAATa                                                                                                         >  1:1245730/1‑88 (MQ=255)                                                                                          ggATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg                                        <  2:223485/139‑1 (MQ=255)                                                                                           gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGt                                       >  1:240070/1‑139 (MQ=255)                                                                                                ttCCACGGACGCAGTAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAGGTCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt                                                     <  2:763377/120‑1 (MQ=255)                                                                                                ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTa                                                                                      <  2:217840/87‑1 (MQ=255)                                                                                                ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTa                                                                                      >  1:217840/1‑87 (MQ=255)                                                                                                ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt                                                     >  1:763377/1‑120 (MQ=255)                                                                                                ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt                                  >  1:1278697/1‑139 (MQ=255)                                                                                                  ccACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                            <  1:149457/65‑1 (MQ=255)                                                                                                  ccACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                            >  2:149457/1‑65 (MQ=255)                                                                                                        aCGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTa                                                                >  1:1239642/1‑101 (MQ=255)                                                                                                        aCGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTa                                                                <  2:1239642/101‑1 (MQ=255)                                                                                                          gcagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat                        >  1:399998/1‑139 (MQ=255)                                                                                                           cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                    <  2:1436904/64‑1 (MQ=255)                                                                                                           cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                    >  1:1436904/1‑64 (MQ=255)                                                                                                           cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCATGGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat                        >  1:616877/1‑139 (MQ=255)                                                                                                           cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt                                                     >  2:357000/1‑109 (MQ=255)                                                                                                           cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt                                                     <  1:357000/109‑1 (MQ=255)                                                                                                                  tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg                                        >  2:428922/1‑116 (MQ=255)                                                                                                                  tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg                                        <  1:428922/116‑1 (MQ=255)                                                                                                                  tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg                                                                     >  2:241179/1‑86 (MQ=255)                                                                                                                  tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg                                                                     <  1:241179/86‑1 (MQ=255)                                                                                                                    gCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                     <  2:223466/54‑1 (MQ=255)                                                                                                                    gCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                     >  1:223466/1‑54 (MQ=255)                                                                                                                     cAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                     >  1:682931/1‑53 (MQ=255)                                                                                                                     cAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                     <  2:682931/53‑1 (MQ=255)                                                                                                                       aCGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCATGGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATc            <  2:616877/139‑1 (MQ=255)                                                                                                                       aCGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCg           <  2:399998/139‑1 (MQ=255)                                                                                                                          cTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGtt        <  1:84891/139‑1 (MQ=255)                                                                                                                           tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa                                                                                            >  1:153199/1‑55 (MQ=255)                                                                                                                           tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa                                                                                            <  2:153199/55‑1 (MQ=255)                                                                                                                           tCCCAAAGAGTAATGAGCTCTTCGGAATCTTCCTGGCGAAATACGCGTATCT‑CCCT‑GGTATTTGTTACCTTTTTCCGGGTTAAAAGGATGATTATGGCTTGAAAGGTCGAATTAGCCAATATCAGCCGAAAAtgcgtag       >  2:1139718/1‑133 (MQ=255)                                                                                                                                aaGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaaa  >  1:391980/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       CCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAA  >  NC_000913/2551961‑2552226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑