Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,307	A→G	E37E (GAA→GAG)	purM →	phosphoribosylaminoimidazole synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,307	0	A	G	100.0%	220.2 / NA	61	E37E (GAA→GAG)	purM	phosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/29);  total (32/29)

TGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NC_000913/2621183‑2621441                                                                                                                             |                                                                                                                                       tGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCt                                                                                                                          >  1:979911/1‑139 (MQ=255)                  aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcgc                                                                                                         >  1:599035/1‑139 (MQ=255)                          aaCCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCa                                                                                                 <  2:162617/139‑1 (MQ=255)                                tctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCg                                                                                           >  2:1403399/1‑139 (MQ=255)                                tctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCg                                                                                           >  2:222430/1‑139 (MQ=255)                                      cTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     >  1:869685/1‑139 (MQ=255)                                              aTGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTg                                                                              <  2:1362264/138‑1 (MQ=255)                                              aTGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTg                                                                              >  1:1362264/1‑138 (MQ=255)                                               tGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGaa                                                                            >  1:1044771/1‑139 (MQ=255)                                                gCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAAc                                                                           >  2:864238/1‑139 (MQ=255)                                                 ccGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             >  2:492344/1‑104 (MQ=255)                                                 ccGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  1:492344/104‑1 (MQ=255)                                                      gtTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGc                                                                     <  2:214017/139‑1 (MQ=255)                                                        tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                             <  1:1399750/129‑1 (MQ=255)                                                        tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                             >  2:1399750/1‑129 (MQ=255)                                                          atatTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGTGGTCTGGGCGGCTTCGGt                                                                                                             <  1:584417/95‑1 (MQ=255)                                                          atatTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGTGGTCTGGGCGGCTTCGGt                                                                                                             >  2:584417/1‑95 (MQ=255)                                                            atTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                               <  2:1044771/139‑1 (MQ=255)                                                               gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             >  1:1486807/1‑90 (MQ=255)                                                               gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  2:1486807/90‑1 (MQ=255)                                                               gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTg                                                            >  1:731177/1‑139 (MQ=255)                                                                 cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcgc                                                                                                         <  2:537547/92‑1 (MQ=255)                                                                 cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcgc                                                                                                         >  1:537547/1‑92 (MQ=255)                                                                     ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTg                                                                      >  1:1015397/1‑123 (MQ=255)                                                                     ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTg                                                                      <  2:1015397/123‑1 (MQ=255)                                                                     ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                               >  2:118468/1‑130 (MQ=255)                                                                     ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                               <  1:118468/130‑1 (MQ=255)                                                                      gTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGa                                                     <  1:864238/139‑1 (MQ=255)                                                                       tAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     <  2:832082/106‑1 (MQ=255)                                                                       tAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     >  1:832082/1‑106 (MQ=255)                                                                            ctctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGt                                               <  2:1377261/139‑1 (MQ=255)                                                                               tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  2:1203514/1‑77 (MQ=255)                                                                               tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  1:1203514/77‑1 (MQ=255)                                                                                   tGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTAcc                                        <  2:869685/139‑1 (MQ=255)                                                                                    ggAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCa                                       >  2:121850/1‑139 (MQ=255)                                                                                      aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                     >  1:942489/1‑139 (MQ=255)                                                                                      aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                     <  1:222430/139‑1 (MQ=255)                                                                                      aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                     <  1:1403399/139‑1 (MQ=255)                                                                                        gaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                   <  2:979911/139‑1 (MQ=255)                                                                                           tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCg                                >  1:1510052/1‑139 (MQ=255)                                                                                             aaaGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTc                              <  2:599035/139‑1 (MQ=255)                                                                                                   gTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCaa                        >  1:841259/1‑139 (MQ=255)                                                                                                    tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                       >  2:1014077/1‑139 (MQ=255)                                                                                                     aGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                <  1:1415167/81‑1 (MQ=255)                                                                                                     aGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                >  2:1415167/1‑81 (MQ=255)                                                                                                     aGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGc                                                                     >  2:1188988/1‑92 (MQ=255)                                                                                                     aGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGc                                                                     <  1:1188988/92‑1 (MQ=255)                                                                                                           gaaAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCaa                        <  2:557836/131‑1 (MQ=255)                                                                                                           gaaAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCaa                        >  1:557836/1‑131 (MQ=255)                                                                                                           gaaAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTa                >  1:138390/1‑139 (MQ=255)                                                                                                            aaaaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGAc                                                    <  2:635558/102‑1 (MQ=255)                                                                                                            aaaaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGAc                                                    >  1:635558/1‑102 (MQ=255)                                                                                                              aaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                   >  2:42535/1‑117 (MQ=255)                                                                                                              aaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                   <  1:42535/117‑1 (MQ=255)                                                                                                                cgcgTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:1425804/44‑1 (MQ=255)                                                                                                                cgcgTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  1:1425804/1‑44 (MQ=255)                                                                                                                  cgtcgtCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     >  1:319045/1‑63 (MQ=255)                                                                                                                  cgtcgtCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     <  2:319045/63‑1 (MQ=255)                                                                                                                   gtcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa        >  2:946314/1‑139 (MQ=255)                                                                                                                      gtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACgt     <  2:942489/139‑2 (MQ=255)                                                                                                                         cGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGacac  <  2:1510052/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                       TGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NC_000913/2621183‑2621441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑