Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,706,482 C→T G215S (GGC→AGC)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,706,4820CT100.0% 152.5 / NA 47G215S (GGC→AGC) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/25);  total (22/25)

GAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG  >  NC_000913/2706351‑2706617
                                                                                                                                   |                                                                                                                                       
gAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTa                                                                                                                                  <  1:467326/139‑1 (MQ=255)
  gccagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAAt                                                                                                                                >  1:829787/1‑139 (MQ=255)
   ccagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  2:155098/139‑1 (MQ=255)
    cagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATAc                                                                                                                              >  1:259691/1‑139 (MQ=255)
       ccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGa                                                                                                                           >  1:892769/1‑139 (MQ=255)
       ccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGa                                                                                                                           >  1:4452/1‑139 (MQ=255)
        cTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          <  2:259691/139‑1 (MQ=255)
                ccACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGaaa                                                                                                                  >  2:379491/1‑139 (MQ=255)
                  acaTTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaaca                                                                                                                <  1:379491/139‑1 (MQ=255)
                     tttCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaac                                                                                                                 <  1:107593/135‑1 (MQ=255)
                     tttCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaac                                                                                                                 >  2:107593/1‑135 (MQ=255)
                       tcagttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           <  2:442342/139‑1 (MQ=255)
                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          <  2:642657/115‑1 (MQ=255)
                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          >  1:642657/1‑115 (MQ=255)
                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACACAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           <  2:86333/130‑1 (MQ=255)
                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACACAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           >  1:86333/1‑130 (MQ=255)
                                          cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        >  2:778291/1‑139 (MQ=255)
                                          cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        <  2:829787/139‑1 (MQ=255)
                                             tttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAg                                                                                     <  1:778291/139‑1 (MQ=255)
                                              ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  1:1183011/96‑1 (MQ=255)
                                              ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               >  2:1183011/1‑96 (MQ=255)
                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  1:734933/87‑1 (MQ=255)
                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               >  2:734933/1‑87 (MQ=255)
                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTa                                                                           <  1:784120/139‑1 (MQ=255)
                                                           tGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGc                                                                       <  2:1220022/139‑1 (MQ=255)
                                                                 gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCaa                                                                 >  2:841398/1‑139 (MQ=255)
                                                                    ggTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  2:1044588/139‑1 (MQ=255)
                                                                              ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                    <  2:892769/139‑1 (MQ=255)
                                                                              ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                    <  2:4452/139‑1 (MQ=255)
                                                                                       cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  2:1270194/120‑1 (MQ=255)
                                                                                       cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  1:1270194/1‑120 (MQ=255)
                                                                                                 aTGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGaa                                 >  1:299102/1‑139 (MQ=255)
                                                                                                      tttCACTTTGTCGCCCTTACGCAGTGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            <  1:841398/139‑1 (MQ=255)
                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        <  1:336644/79‑1 (MQ=255)
                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        >  2:336644/1‑79 (MQ=255)
                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  1:37110/105‑1 (MQ=255)
                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  2:37110/1‑105 (MQ=255)
                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACgg                                                                                                                            <  1:1120683/41‑1 (MQ=255)
                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACgg                                                                                                                            >  2:1120683/1‑41 (MQ=255)
                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            <  2:589409/137‑1 (MQ=255)
                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            >  1:589409/1‑137 (MQ=255)
                                                                                                         cACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                           <  2:418441/105‑1 (MQ=255)
                                                                                                         cACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                           >  1:418441/1‑105 (MQ=255)
                                                                                                               gTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  1:9059/96‑1 (MQ=255)
                                                                                                               gTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  2:9059/1‑96 (MQ=255)
                                                                                                                              ggTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc    >  1:48530/1‑139 (MQ=255)
                                                                                                                                tGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTg  >  2:998644/1‑139 (MQ=255)
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GAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG  >  NC_000913/2706351‑2706617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑