Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,132	T→C	D532G (GAC→GGC)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,132	0	T	C	100.0%	219.6 / NA	60	D532G (GAC→GGC)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/32);  total (28/32)

CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAG  >  NC_000913/2780998‑2781259                                                                                                                                       |                                                                                                                                cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                             >  2:502604/1‑139 (MQ=255) cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                             >  1:870365/1‑139 (MQ=255)       tAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTc                                                                                                                       <  2:288431/139‑1 (MQ=255)         gCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCa                                                                                                                     >  2:649909/1‑139 (MQ=255)           ttCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGa                                                                                                                   >  1:1239395/1‑139 (MQ=255)                   tCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                           <  1:229709/139‑1 (MQ=255)                             gAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                             >  2:39012/1‑127 (MQ=255)                             gAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                             <  1:39012/127‑1 (MQ=255)                                aGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAATCTg                                                                                              >  1:785711/1‑139 (MQ=255)                                   aTCACCATAACTGTTGGCTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCAACGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGtt                                                                                           <  1:926287/139‑2 (MQ=255)                                     cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                             <  2:1366753/103‑1 (MQ=255)                                     cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                             >  1:1366753/1‑103 (MQ=255)                                               gTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                            >  1:505742/1‑94 (MQ=255)                                               gTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                            <  2:505742/94‑1 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTTGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACCTGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGt                                                                                                 >  2:377001/1‑120 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGt                                                                                                 <  1:377001/120‑1 (MQ=255)                                                ttGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAg                                                                              >  1:677541/1‑139 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       <  1:250260/128‑1 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       >  2:250260/1‑128 (MQ=255)                                                  ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            <  1:649909/139‑1 (MQ=255)                                                     tCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       >  1:345854/1‑125 (MQ=255)                                                     tCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       <  2:345854/125‑1 (MQ=255)                                                     tCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcctcc                                                                         >  1:265261/1‑139 (MQ=255)                                                        ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTc                                                                      >  2:1410904/1‑139 (MQ=255)                                                                 tgtTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                             <  2:1239395/139‑1 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            >  1:631864/1‑122 (MQ=255)                                                                   ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            <  2:631864/122‑1 (MQ=255)                                                                           gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGc                                                   <  2:161718/139‑1 (MQ=255)                                                                            tCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                           <  1:1388173/82‑1 (MQ=255)                                                                            tCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                           >  2:1388173/1‑82 (MQ=255)                                                                              gTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                            <  1:433338/63‑1 (MQ=255)                                                                              gTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                            >  2:433338/1‑63 (MQ=255)                                                                                     cAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                               >  1:1137513/1‑69 (MQ=255)                                                                                     cAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                               <  2:1137513/69‑1 (MQ=255)                                                                                            tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            >  2:907541/1‑97 (MQ=255)                                                                                            tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            <  1:907541/97‑1 (MQ=255)                                                                                                ccgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            >  1:1359870/1‑93 (MQ=255)                                                                                                ccgccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                            <  2:1359870/93‑1 (MQ=255)                                                                                                     ggATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAATCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGcc                         <  2:785711/139‑1 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                        <  1:554296/139‑1 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                        <  2:468030/139‑1 (MQ=255)                                                                                                      gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCCGGACAATCAGCTTACTGCCAGTATCAATGGTAGTATTTTTCGCCg                        <  2:870365/139‑1 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       <  2:147590/71‑1 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                       >  1:147590/1‑71 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTg                                                                    >  2:1123990/1‑90 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTg                                                                    <  1:1123990/90‑1 (MQ=255)                                                                                                           ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGtt                   <  1:1410904/139‑1 (MQ=255)                                                                                                               tCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                 <  1:557412/105‑1 (MQ=255)                                                                                                               tCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                 >  2:557412/1‑105 (MQ=255)                                                                                                                   cATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                           <  2:407504/59‑1 (MQ=255)                                                                                                                   cATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                           >  1:407504/1‑59 (MQ=255)                                                                                                                    aTGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACt          <  2:677541/139‑1 (MQ=255)                                                                                                                      gCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAAt                                                              >  2:1147163/1‑85 (MQ=255)                                                                                                                      gCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAAt                                                              <  1:1147163/85‑1 (MQ=255)                                                                                                                           gCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                           >  2:958494/1‑35 (MQ=255)                                                                                                                           gCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                           <  1:958494/35‑1 (MQ=255)                                                                                                                           gCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCa   >  1:1300203/1‑139 (MQ=255)                                                                                                                            ccTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAg  <  1:502604/139‑1 (MQ=255)                                                                                                                                 ccaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTgg                                                                   <  2:561684/69‑1 (MQ=255)                                                                                                                                 ccaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTgg                                                                   >  1:561684/1‑69 (MQ=255)                                                                                                                                       |                                                                                                                                CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAG  >  NC_000913/2780998‑2781259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑