Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	253,084	A→G	T126A (ACA→GCA)	yafP →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	253,084	0	A	G	100.0%	107.5 / NA	31	T126A (ACA→GCA)	yafP	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

CTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGA  >  NC_000913/252962‑253212                                                                                                                           |                                                                                                                                 cTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAgcg                                                                                                                  >  1:909826/1‑139 (MQ=255)          cccGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATg                                                                                                         >  1:22189/1‑139 (MQ=255)              ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                     >  2:533080/1‑139 (MQ=255)              ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                     >  1:725301/1‑139 (MQ=255)                 tGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGa                                                                                                  <  1:308006/139‑1 (MQ=255)                      ttGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                                             <  2:22189/139‑1 (MQ=255)                      ttGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTCTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                                             <  1:985279/139‑1 (MQ=255)                               cTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGAAAGCATAACCGCAAAACCATTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCAGGGGAGCGTGGTTTACTaa                                                                                    >  2:670876/1‑139 (MQ=255)                                         aCCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATg                                                                          <  1:533080/139‑1 (MQ=255)                                            tttGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGa                                                                       >  2:1458985/1‑139 (MQ=255)                                              tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGata                                                                     >  1:114702/1‑139 (MQ=255)                                                     gTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCg                                                              <  2:106790/139‑1 (MQ=255)                                                          aaTCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACa                                                         >  1:590933/1‑139 (MQ=255)                                                                aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                                            >  2:804289/1‑130 (MQ=255)                                                                aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                                            <  1:804289/130‑1 (MQ=255)                                                                             cGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATg                                      <  2:909826/139‑1 (MQ=255)                                                                                aaGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATg                                                                          >  1:619840/1‑100 (MQ=255)                                                                                aaGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATg                                                                          <  2:619840/100‑1 (MQ=255)                                                                                   cATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAATATTAAATCCAGCTTGCAATGAAAATa                                >  2:1239546/1‑139 (MQ=255)                                                                                      aaCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                             <  2:590933/139‑1 (MQ=255)                                                                                        ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAATATTAAATCCAGCTTGCAATGAAAATAAcgcc                           <  1:1239546/139‑1 (MQ=255)                                                                                        ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc                           <  1:1458985/139‑1 (MQ=255)                                                                                          gCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                             <  1:753416/135‑1 (MQ=255)                                                                                          gCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                             >  2:753416/1‑135 (MQ=255)                                                                                             aaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGGGCGTTGa                                                                                                            >  2:870070/1‑53 (MQ=255)                                                                                             aaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGa                                                                                                            <  1:870070/53‑1 (MQ=255)                                                                                                     ttttGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTAtgtg              <  1:670876/139‑1 (MQ=255)                                                                                                               tATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCt    >  1:73367/1‑139 (MQ=255)                                                                                                                 tGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGa  >  2:569070/1‑139 (MQ=255)                                                                                                                      ttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                             >  2:1286930/1‑107 (MQ=255)                                                                                                                      ttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                             <  1:1286930/107‑1 (MQ=255)                                                                                                                           |                                                                                                                                 CTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGA  >  NC_000913/252962‑253212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑