Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,491 C→T *362* (TAG→TAA mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4910CT100.0% 73.6 / NA 24*362* (TAG→TAAmltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/12);  total (12/12)

TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGT  >  NC_000913/2824356‑2824616
                                                                                                                                       |                                                                                                                             
tGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                                            >  2:1319207/1‑139 (MQ=255)
                 tGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                                          <  2:829055/139‑1 (MQ=255)
                         aaGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTg                                                                                                  <  1:1319207/139‑1 (MQ=255)
                                cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAAc                                                                                           >  1:1006823/1‑139 (MQ=255)
                                   tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGc                                                                                        <  2:1006823/139‑1 (MQ=255)
                                                 tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑‑CCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGt                                                                        >  2:1063258/1‑139 (MQ=255)
                                                       tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                                      >  1:1049706/1‑105 (MQ=255)
                                                       tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                                      <  2:1049706/105‑1 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCt                                                                                                    >  2:417231/1‑100 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCt                                                                                                    <  1:417231/100‑1 (MQ=255)
                                                                      aCGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTa                                                     <  1:611780/139‑1 (MQ=255)
                                                                              gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTGCTAACTGCCAGACCGCCATTGCGt                                                                        <  2:1022643/112‑1 (MQ=255)
                                                                              gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGt                                                                        >  1:1022643/1‑112 (MQ=255)
                                                                                     aaaaaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                     >  2:286851/1‑76 (MQ=255)
                                                                                     aaaaaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                     <  1:286851/76‑1 (MQ=255)
                                                                                       aaaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACtta                                                                                                                         >  1:814768/1‑53 (MQ=255)
                                                                                       aaaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACtta                                                                                                                         <  2:814768/55‑3 (MQ=255)
                                                                                               aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                             <  1:776641/90‑1 (MQ=255)
                                                                                               aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                             >  2:776641/1‑90 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                                  >  1:469809/1‑87 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                                  <  2:469809/87‑1 (MQ=255)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAtt                                                                            <  2:1362236/72‑1 (MQ=255)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAtt                                                                            >  1:1362236/1‑72 (MQ=255)
                                                                                                                         ccTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGt  >  2:959088/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                             
TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGT  >  NC_000913/2824356‑2824616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: