Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,873	G→A	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB → hypC →	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,873	0	G	A	100.0%	107.0 / NA	34	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB hypC	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

GACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGG  >  NC_000913/2851745‑2852005                                                                                                                                 |                                                                                                                                     gACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCgg                                                                                                                            >  2:641495/1‑139 (MQ=255)  gccttGAAAAGTTGATCGCCTGCCCCCGCGAAGTCAAACCAAGAATTGGAATCATTCTTATTTTCGCCACCCGGGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGAAAAAACGATGGGCATAAGCGTTCCCGGc                                                                                                                           <  1:1277872/136‑1 (MQ=255)             tGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                <  1:1110671/123‑1 (MQ=255)             tGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                >  2:1110671/1‑123 (MQ=255)                                           gAAATTGATATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCTATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                  <  1:897459/139‑1 (MQ=255)                                                  aaaTCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGctc                                                                                                                <  2:207566/102‑3 (MQ=255)                                                  aaaTCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGctc                                                                                                                >  1:207566/1‑100 (MQ=255)                                                      catcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTc                                                                       <  1:641495/139‑1 (MQ=255)                                                         ccatATTTCCGACACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGTAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg                                                                             >  2:873507/4‑130 (MQ=255)                                                         ccTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAg                                                                                                                        <  2:912553/87‑1 (MQ=255)                                                         ccTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAg                                                                                                                        >  1:912553/1‑87 (MQ=255)                                                         ccTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg                                                                             <  1:873507/130‑1 (MQ=255)                                                                   gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                  >  2:1109215/1‑115 (MQ=255)                                                                   gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                  <  1:1109215/115‑1 (MQ=255)                                                                     caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACgg                                                                                                       <  2:729837/92‑1 (MQ=255)                                                                     caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACgg                                                                                                       >  1:729837/1‑92 (MQ=255)                                                                        caGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                  >  2:965833/1‑110 (MQ=255)                                                                        caGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                  <  1:965833/110‑1 (MQ=255)                                                                                             gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTa                                                >  1:1448282/1‑123 (MQ=255)                                                                                             gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTa                                                <  2:1448282/123‑1 (MQ=255)                                                                                             gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATg                                >  1:1257123/1‑139 (MQ=255)                                                                                              tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTGTGCGGCAtt                                                                        >  2:626914/1‑98 (MQ=255)                                                                                              tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCAtt                                                                        <  1:626914/98‑1 (MQ=255)                                                                                              tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                               >  2:188243/1‑139 (MQ=255)                                                                                                     aCTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                >  2:1486523/1‑35 (MQ=255)                                                                                                     aCTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                <  1:1486523/35‑1 (MQ=255)                                                                                                                 cacaGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTg            <  2:1257123/139‑1 (MQ=255)                                                                                                                   caGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                            >  2:154645/1‑57 (MQ=255)                                                                                                                   caGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                            <  1:154645/57‑1 (MQ=255)                                                                                                                           tgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                            <  2:1324677/49‑1 (MQ=255)                                                                                                                           tgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                            >  1:1324677/1‑49 (MQ=255)                                                                                                                           tgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCa       <  2:527618/134‑1 (MQ=255)                                                                                                                           tgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCa       >  1:527618/1‑134 (MQ=255)                                                                                                                           tgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTggg  >  1:205337/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     GACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGG  >  NC_000913/2851745‑2852005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑