Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,563	G→A	R354H (CGT→CAT)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,563	0	G	A	100.0%	159.2 / NA	49	R354H (CGT→CAT)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

TGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGG  >  NC_000913/262429‑262695                                                                                                                                       |                                                                                                                                     tGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCAtttt                                                                                                                                  >  2:575520/1‑139 (MQ=255)       tGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAAc                                                                                                                            >  2:997363/1‑139 (MQ=255)         aGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGa                                                                                                                          >  2:527678/1‑139 (MQ=255)             tCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTg                                                                                                                      <  1:575520/139‑1 (MQ=255)                       gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAATACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  2:1179741/139‑1 (MQ=255)                             aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                      >  1:1418750/1‑139 (MQ=255)                                  cAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGtt                                                                                                 <  2:1433822/139‑1 (MQ=255)                                                   ttGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGttt                                                                                >  1:1269039/1‑139 (MQ=255)                                                    tGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGtttt                                                                               >  2:899010/1‑139 (MQ=255)                                                        gATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCg                                                                           >  1:672285/1‑139 (MQ=255)                                                           gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGa                                                                                                                    >  1:541162/1‑95 (MQ=255)                                                           gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGa                                                                                                                    <  2:541162/95‑1 (MQ=255)                                                               tCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                    <  2:1269039/139‑1 (MQ=255)                                                                          cGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTc                                                         >  2:549/1‑139 (MQ=255)                                                                           gTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCt                                                        <  2:1418750/139‑1 (MQ=255)                                                                             gAACACGGCACACAACATTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              >  1:943450/1‑67 (MQ=255)                                                                             gAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGGCCAGCATTTTGTTAACGAAGTGGATTc                                                                                                                 <  1:976228/80‑1 (MQ=255)                                                                             gAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              <  2:943450/67‑1 (MQ=255)                                                                             gAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCGTCTACGCGTTGTACCGACGGCGGCCAGTTTGGTCTgg                                                      >  2:959251/1‑139 (MQ=255)                                                                             gAACACGGCACACAACACTCCGATGCGATCCTGAACCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTc                                                                                                                 >  2:976228/1‑80 (MQ=255)                                                                                   ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCgg                                                <  1:899010/139‑1 (MQ=255)                                                                                             acTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             >  1:1162794/1‑52 (MQ=255)                                                                                             acTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             <  2:1162794/52‑1 (MQ=255)                                                                                               tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                    >  1:945958/1‑107 (MQ=255)                                                                                               tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                    <  2:945958/107‑1 (MQ=255)                                                                                               tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTaa                                    >  2:210323/1‑139 (MQ=255)                                                                                                  gATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAc                                                                                      <  2:1199620/86‑1 (MQ=255)                                                                                                  gATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAc                                                                                      >  1:1199620/1‑86 (MQ=255)                                                                                                      cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACTGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGcacaca                             >  2:1410521/1‑139 (MQ=255)                                                                                                        aTCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaa                           >  1:1390416/1‑139 (MQ=255)                                                                                                         tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGt                                                                                                  >  1:1042051/1‑67 (MQ=255)                                                                                                         tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGt                                                                                                  <  2:1042051/67‑1 (MQ=255)                                                                                                         tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACATTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          <  1:864562/107‑1 (MQ=255)                                                                                                         tCCTGACCCGCGATATGAGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACATTAACACCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          >  2:864562/1‑107 (MQ=255)                                                                                                              aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTcc                     >  1:1452825/1‑139 (MQ=255)                                                                                                               cccGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                    <  2:672285/139‑1 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACg                                                                                             >  2:186158/1‑62 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACg                                                                                             <  1:186158/62‑1 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCg                                                                           >  1:556408/1‑80 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCg                                                                           <  2:556408/80‑1 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGt                                      >  1:736794/1‑117 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGt                                      <  2:736794/117‑1 (MQ=255)                                                                                                                   cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg                >  1:1080746/1‑139 (MQ=255)                                                                                                                     atatGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgcg              <  1:997363/139‑1 (MQ=255)                                                                                                                          cgcAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  2:743923/40‑1 (MQ=255)                                                                                                                          cgcAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            >  1:743923/1‑40 (MQ=39)                                                                                                                             aaCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGATTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCaa      <  2:1080746/139‑1 (MQ=255)                                                                                                                              aCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAAt     <  1:210323/139‑1 (MQ=255)                                                                                                                                 cccAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATggg  <  1:527678/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGG  >  NC_000913/262429‑262695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑