Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	*279* (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	214.2 / NA	66	*279* (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/34);  total (32/34)

ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGA  >  NC_000913/2983154‑2983398                                                                                                                                       |                                                                                                               acaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgc                                                                                                            >  2:804138/1‑139 (MQ=255)   aGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgcgc                                                                                                          >  1:1069472/1‑139 (MQ=255)   aGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAgcgc                                                                                                          <  2:805348/139‑1 (MQ=255)             cgcCAGCCCCAACGCCATCCCCT‑ACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAAc                                                                                               >  2:51863/1‑139 (MQ=255)                 acccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGc                                                                                            >  1:1476018/3‑139 (MQ=255)                     ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        <  2:1145294/139‑1 (MQ=255)                                          gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAAc                                                                                               <  1:1318674/111‑1 (MQ=255)                                          gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAAc                                                                                               >  2:1318674/1‑111 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        <  1:537152/117‑1 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        >  2:537152/1‑117 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATGATCAAAGACCt                                                                  >  1:978516/1‑139 (MQ=255)                                           tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                  >  1:670873/1‑139 (MQ=255)                                                tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAAACCTGGttt                                                             >  2:1256009/1‑139 (MQ=255)                                                 aTCACAACCAGTGACGACCTCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                            >  2:185648/1‑139 (MQ=255)                                                    acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        >  2:940396/1‑108 (MQ=255)                                                    acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        <  1:940396/108‑1 (MQ=255)                                                    acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                         <  2:1069472/139‑1 (MQ=255)                                                    acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                         <  2:1476018/139‑1 (MQ=255)                                                    acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                         <  1:804138/139‑1 (MQ=255)                                                          aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                   >  1:662945/1‑139 (MQ=255)                                                           gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        <  2:522638/101‑1 (MQ=255)                                                           gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        >  1:522638/1‑101 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACAt                                                                                       <  2:1406986/101‑1 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACAt                                                                                       >  1:1406986/1‑101 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                    >  2:94656/1‑120 (MQ=255)                                                            tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                    <  1:94656/120‑1 (MQ=255)                                                              acgacCTCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                               <  1:185648/139‑1 (MQ=255)                                                              acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                               <  1:51863/139‑1 (MQ=255)                                                                            aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCat                                                                                <  2:1357466/92‑1 (MQ=255)                                                                            aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCat                                                                                >  1:1357466/1‑92 (MQ=255)                                                                                       aaaaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAACTCTTTAACGGCcac                                                                                         <  1:914839/72‑1 (MQ=255)                                                                                       aaaaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                         >  2:914839/1‑72 (MQ=255)                                                                                             cACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                <  2:978516/139‑1 (MQ=255)                                                                                             cACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                <  2:1265285/139‑1 (MQ=255)                                                                                             cACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                <  2:662945/139‑1 (MQ=255)                                                                                                 tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                              >  2:479949/1‑105 (MQ=255)                                                                                                 tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                              <  1:479949/105‑1 (MQ=255)                                                                                                  aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                         >  2:641721/1‑61 (MQ=255)                                                                                                  aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                         <  1:641721/61‑1 (MQ=255)                                                                                                       cATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGa      <  1:1256009/139‑1 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                    <  1:388848/85‑1 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                               >  2:460170/1‑90 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                               <  1:460170/90‑1 (MQ=255)                                                                                                               tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATAATCAAAGACCTGGTTTTCACCGAcc                                                    >  2:388848/1‑85 (MQ=255)                                                                                                                 ccTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATgg                                                                                     <  1:1507240/50‑1 (MQ=255)                                                                                                                 ccTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATgg                                                                                     >  2:1507240/1‑50 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                               <  2:1209884/53‑1 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                               >  1:1209884/1‑53 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                 >  2:472448/1‑83 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                 <  1:472448/83‑1 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa  >  2:429807/1‑130 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa  <  1:429807/130‑1 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa  <  1:660265/130‑1 (MQ=255)                                                                                                                    tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa  >  2:660265/1‑130 (MQ=255)                                                                                                                     aTTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        <  2:137925/43‑1 (MQ=255)                                                                                                                     aTTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                        >  1:137925/1‑43 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                             <  1:1182809/37‑1 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                             >  2:1182809/1‑37 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa           <  2:635887/119‑1 (MQ=255)                                                                                                                      ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa           >  1:635887/1‑119 (MQ=255)                                                                                                                       tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGa      >  2:1284103/1‑123 (MQ=255)                                                                                                                       tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGa      <  1:1284103/123‑1 (MQ=255)                                                                                                                           ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                    >  2:1191478/1‑73 (MQ=255)                                                                                                                           ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                    <  1:1191478/73‑1 (MQ=255)                                                                                                                                   acaATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                  <  2:1249190/83‑1 (MQ=255)                                                                                                                                   acaATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                  >  1:1249190/1‑83 (MQ=255)                                                                                                                                       |                                                                                                               ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGA  >  NC_000913/2983154‑2983398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑