Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,021	T→C	Y35C (TAC→TGC)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,021	0	T	C	100.0%	220.3 / NA	61	Y35C (TAC→TGC)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

AACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAA  >  NC_000913/2983892‑2984154                                                                                                                                  |                                                                                                                                      aaCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCgg                                                                                                                              >  2:1409738/1‑139 (MQ=255)       cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                        >  2:118625/1‑139 (MQ=255)       cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACaa                                                                                                                        >  2:673043/1‑139 (MQ=255)            ttCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATAcc                                                                                                                   >  2:1388492/1‑139 (MQ=255)            ttCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATAcc                                                                                                                   >  1:738580/1‑139 (MQ=255)              cAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCtt                                                                                                                 >  2:1145810/1‑139 (MQ=255)                aGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCtttt                                                                                                               <  1:118625/139‑1 (MQ=255)                      tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                         >  2:743476/1‑139 (MQ=255)                      tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCa                                                                                                         >  1:527075/1‑139 (MQ=255)                           tACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAAt                                                                                                    >  1:169868/1‑139 (MQ=255)                           tACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAAt                                                                                                    >  2:1346288/1‑139 (MQ=255)                             cGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTc                                                                                                  >  1:1403708/1‑139 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCa                                                                                                             <  1:1379280/122‑1 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCa                                                                                                             >  2:1379280/1‑122 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  2:1323460/139‑1 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:252835/139‑1 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:537068/139‑1 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:743476/139‑1 (MQ=255)                                   gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  2:1403708/139‑1 (MQ=255)                                              aCTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTggg                                                                                 >  1:730992/1‑139 (MQ=255)                                                 tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTAt                                                                              >  2:1259969/1‑139 (MQ=255)                                                          aCGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGttt                                                                     >  1:495000/1‑139 (MQ=255)                                                             gAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtg                                                                  <  2:221436/139‑1 (MQ=255)                                                                   gTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgtgcg                                                            <  1:1259969/139‑1 (MQ=255)                                                                    ttttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTgcgc                                                           <  1:1409738/139‑1 (MQ=255)                                                                     tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGTGTgcgct                                                          >  2:952001/1‑138 (MQ=255)                                                                     tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCa                                                          <  2:738580/139‑1 (MQ=255)                                                                      ttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCAc                                                         <  2:495000/139‑1 (MQ=255)                                                                      ttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCAc                                                         <  1:804352/139‑1 (MQ=255)                                                                          ttATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACtgtg                                                     <  1:1145810/139‑1 (MQ=255)                                                                            aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTgcgc                                                           >  2:169196/1‑131 (MQ=255)                                                                            aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTgcgc                                                           <  1:169196/131‑1 (MQ=255)                                                                            aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTgcgc                                                           >  2:909226/1‑131 (MQ=255)                                                                            aTCGGCATAATGCCGCCAACAATAATTAGGTCAATGTGGCTGTAAACCATGGTGCGCTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTgcgc                                                           <  1:909226/131‑1 (MQ=255)                                                                                gCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGct                                               <  1:673043/139‑1 (MQ=255)                                                                                 cATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGctc                                              <  2:169868/139‑1 (MQ=255)                                                                                    aaTGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCAc                                                         <  1:1504225/125‑1 (MQ=255)                                                                                    aaTGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCAc                                                         >  2:1504225/1‑125 (MQ=255)                                                                                        ccgccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  2:280897/86‑1 (MQ=255)                                                                                        ccgccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            >  1:280897/1‑86 (MQ=255)                                                                                         cgccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTAc                                      <  1:1346288/139‑1 (MQ=255)                                                                                           ccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCa                                                                                          <  2:24881/85‑1 (MQ=255)                                                                                           ccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCa                                                                                          >  1:24881/1‑85 (MQ=255)                                                                                                aataatTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGc                                                >  1:1231001/1‑122 (MQ=255)                                                                                                aataatTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGc                                                <  2:1231001/122‑1 (MQ=255)                                                                                                     ttCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                          <  2:527075/139‑1 (MQ=255)                                                                                                      gcggtataTGGGGCTAAAACCCATTGTGCTCTCAGCGGACACAAATACCTTTACCACAAAATTCTTATCGCGCAGCCCTTGGGTATCCA‑‑GTTTTTTTGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTc                       <  1:952001/132‑1 (MQ=255)                                                                                                         gTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAGATACCTTTTCAACCAAAAATTCATTgcg                                                                                            >  2:914442/1‑69 (MQ=255)                                                                                                         gTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:914442/69‑1 (MQ=255)                                                                                                          tCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                     >  1:1402914/1‑139 (MQ=255)                                                                                                          tCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                     <  2:730992/139‑1 (MQ=255)                                                                                                             aTGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            >  2:1292596/1‑65 (MQ=255)                                                                                                             aTGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:1292596/65‑1 (MQ=255)                                                                                                                      tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCt                                         <  2:829028/107‑1 (MQ=255)                                                                                                                      tAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCt                                         >  1:829028/1‑107 (MQ=255)                                                                                                                        aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTtgcgt                                                               <  1:1079932/83‑1 (MQ=255)                                                                                                                        aaCCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTCTtgcgt                                                               >  2:1079932/1‑83 (MQ=255)                                                                                                                            aTGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                     >  2:680080/1‑121 (MQ=255)                                                                                                                            aTGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                     <  1:680080/121‑1 (MQ=255)                                                                                                                             tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            >  2:364755/1‑49 (MQ=255)                                                                                                                             tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                            <  1:364755/49‑1 (MQ=255)                                                                                                                             tGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaa  >  2:396304/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      AACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAA  >  NC_000913/2983892‑2984154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑