Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,995,314 C→T pseudogene (447/447 nt) ygeN ← pseudogene, orgB family, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,995,3140CT100.0% 85.5 / NA 28pseudogene (447/447 nt)ygeNpseudogene, orgB family, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

AATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGG  >  NC_000913/2995197‑2995432
                                                                                                                      |                                                                                                                      
aaTATAATTAAAAATAAAATTTTTGCGTAAAAAAAATACCACAGGCATTAAAAAATCATGAGGTGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGc                                                                                                    <  2:1081684/139‑1 (MQ=255)
                 aatTTTTGCGTAAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCa                                                                                   <  1:1222622/139‑1 (MQ=255)
                                        acaGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAAc                                                            >  2:344873/1‑139 (MQ=255)
                                              aTTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACAttttt                                                      >  2:250617/1‑139 (MQ=255)
                                                                           tACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATaa                                                                                                                   >  2:520491/1‑49 (MQ=255)
                                                                           tACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATaa                                                                                                                   <  1:520491/49‑1 (MQ=255)
                                                                           tACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAAc                                                 <  1:1166506/115‑1 (MQ=255)
                                                                           tACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAAc                                                 >  2:1166506/1‑115 (MQ=255)
                                                                                             aaaTCATTGAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGaa                                                                                          >  1:979920/1‑56 (MQ=255)
                                                                                             aaaTCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGaa                                                                                          <  2:979920/56‑1 (MQ=255)
                                                                                              aaTCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCa                                                               >  1:168840/1‑82 (MQ=255)
                                                                                              aaTCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCa                                                               <  2:168840/82‑1 (MQ=255)
                                                                                                  aTTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg  >  1:245/1‑139 (MQ=255)
                                                                                                  aTTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAgggg  <  2:245/139‑1 (MQ=255)
                                                                                                    tAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAattatt                                                                                     >  1:467363/1‑54 (MQ=255)
                                                                                                    tAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAattatt                                                                                     <  2:467363/54‑1 (MQ=255)
                                                                                                           gAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAattatt                                                                                     <  1:885730/47‑1 (MQ=255)
                                                                                                           gAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCa                                                                             >  1:409525/1‑55 (MQ=255)
                                                                                                           gAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCa                                                                             <  2:409525/55‑1 (MQ=255)
                                                                                                           gAGCCAGGGAGTTATAAGTCATCAGGGCTTGCCAAAACAGAattatt                                                                                     >  2:885730/1‑47 (MQ=255)
                                                                                                            aGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATGCAAGAATGTGTTTCAAACATTTTTTCAACt                                                <  1:1074915/82‑1 (MQ=255)
                                                                                                            aGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAAt                                                                       >  1:512849/1‑60 (MQ=255)
                                                                                                            aGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAAt                                                                       <  2:512849/60‑1 (MQ=255)
                                                                                                            aGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAGAATGTGTTTCAAACATTTTTTCAACt                                                >  2:1074915/1‑82 (MQ=255)
                                                                                                                 gggAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACAtttttt                                                     >  2:712166/1‑73 (MQ=255)
                                                                                                                 gggAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACAtttttt                                                     <  1:712166/73‑1 (MQ=255)
                                                                                                                  ggAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAAGCTTGTCAg     <  1:979492/120‑1 (MQ=255)
                                                                                                                  ggAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTACGCAGATTTCGTTAACCTTGTCAg     >  2:979492/1‑120 (MQ=255)
                                                                                                                      |                                                                                                                      
AATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCCAAAGAATGTGTTTCAAACATTTTTTCAACTAAATAATGTCTGGCTTGTTCGCAGATTTCGTTAACCTTGTCAGGGG  >  NC_000913/2995197‑2995432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: