Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,059,293	T→C	G11G (GGA→GGG)	rpiA ←	ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,059,293	0	T	C	100.0%	242.5 / NA	68	G11G (GGA→GGG)	rpiA	ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059167‑3059427                                                                                                                               |                                                                                                                                       tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCAAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                            <  2:860708/139‑1 (MQ=255)            cGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATc                                                                                                                 <  1:587533/139‑1 (MQ=255)              gCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  1:1460841/139‑1 (MQ=255)                cccTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCGACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  2:898737/137‑1 (MQ=255)                cccTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               >  1:898737/1‑137 (MQ=255)                            gCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGtt                                                                                                 >  2:1342371/1‑139 (MQ=255)                             ccTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGttt                                                                                                >  1:98202/1‑139 (MQ=255)                                         ccGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTAt                                                                                    >  1:972214/1‑139 (MQ=255)                                            aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:1212038/1‑139 (MQ=255)                                               gcgTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAAtt                                                                              >  2:579386/1‑139 (MQ=255)                                                 gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  2:150275/1‑139 (MQ=255)                                                 gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  1:684683/1‑139 (MQ=255)                                                   cAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:98202/139‑1 (MQ=255)                                                      tAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             >  2:736523/1‑117 (MQ=255)                                                      tAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             <  1:736523/117‑1 (MQ=255)                                                          gtgtGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAtt                                                                   <  2:972214/139‑1 (MQ=255)                                                          gtgtGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAtt                                                                   <  1:1342371/139‑1 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  2:1298343/113‑1 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  1:1298343/1‑113 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:525882/113‑1 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:525882/1‑113 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  2:693489/1‑129 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  1:693489/129‑1 (MQ=255)                                                             tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                >  1:1355509/1‑139 (MQ=255)                                                                       gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                       <  2:859001/90‑1 (MQ=255)                                                                       gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                       >  1:859001/1‑90 (MQ=255)                                                                                 cTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            >  1:1095650/1‑139 (MQ=255)                                                                                 cTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            <  2:684683/139‑1 (MQ=255)                                                                                  tACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAc                                           <  2:434328/139‑1 (MQ=255)                                                                                   acacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  2:654581/1‑105 (MQ=255)                                                                                   acacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            <  1:654581/105‑1 (MQ=255)                                                                                    cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                           <  1:1466911/57‑1 (MQ=255)                                                                                    cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                           >  2:1466911/1‑57 (MQ=255)                                                                                    cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAccc                                         <  2:938102/139‑1 (MQ=255)                                                                                    cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCAGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAccc                                         >  1:938102/1‑139 (MQ=255)                                                                                        aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             >  1:1294736/1‑83 (MQ=255)                                                                                        aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             <  2:1294736/83‑1 (MQ=255)                                                                                         acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                <  2:207283/63‑1 (MQ=255)                                                                                         acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                >  1:207283/1‑63 (MQ=255)                                                                                           aaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  2:29672/62‑1 (MQ=255)                                                                                           aaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               >  1:29672/1‑62 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAAtt                                                                                                                     >  1:741491/1‑52 (MQ=255)                                                                                               gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAAtt                                                                                                                     <  2:741491/52‑1 (MQ=255)                                                                                                tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:1267214/94‑1 (MQ=255)                                                                                                tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  1:1267214/1‑94 (MQ=255)                                                                                                tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGcc                             <  1:579386/139‑1 (MQ=255)                                                                                                ggCCCGGCTGAACATACTGAAGTGTCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGcg                             <  2:1095650/138‑2 (MQ=255)                                                                                                 gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            >  1:329623/1‑139 (MQ=255)                                                                                                  ccGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                           <  2:1355509/139‑1 (MQ=255)                                                                                                          aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGtt                     <  2:1314340/137‑1 (MQ=255)                                                                                                          aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGtt                     >  1:1314340/1‑137 (MQ=255)                                                                                                           aCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAAc                  <  2:1212038/139‑1 (MQ=255)                                                                                                               aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt              <  1:150275/139‑1 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:256204/58‑1 (MQ=255)                                                                                                                    aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:256204/1‑58 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtgtg                                                                                        >  2:1277017/1‑56 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtgtg                                                                                        <  1:1277017/56‑1 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           <  1:1130681/53‑1 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           >  2:1130681/1‑53 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            <  2:660219/100‑1 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            >  1:660219/1‑100 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAat     >  2:384364/1‑139 (MQ=255)                                                                                                                        ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAat     >  1:977359/1‑139 (MQ=255)                                                                                                                         cgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGTAAGCcgc                           <  1:922006/116‑1 (MQ=255)                                                                                                                         cgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                           >  2:922006/1‑116 (MQ=255)                                                                                                                           cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            >  1:1142565/1‑113 (MQ=255)                                                                                                                           cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            <  2:1142565/113‑1 (MQ=255)                                                                                                                           cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATc  >  1:392987/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                       TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059167‑3059427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑