Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,081,877 T→C intergenic (‑242/‑36) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein, low salt‑inducible, Era‑binding heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,081,8770TC100.0% 304.3 / NA 82intergenic (‑242/‑36)tktA/loiPtransketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein, low salt‑inducible, Era‑binding heat shock protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/40);  total (42/40)

TGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAA  >  NC_000913/3081750‑3082011
                                                                                                                               |                                                                                                                                      
tGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATc                                                                                                                             >  1:354019/1‑139 (MQ=255)
 ggAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGATACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATcc                                                                                                                            >  2:1489953/1‑139 (MQ=255)
  gAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCt                                                                                                                           >  2:335828/1‑139 (MQ=255)
      aTCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGAcc                                                                                                                       >  1:644995/1‑139 (MQ=255)
           tttGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  >  2:1332110/1‑139 (MQ=255)
            ttGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCtt                                                                                                                     <  2:1044357/135‑1 (MQ=255)
            ttGCCGCTTCAGAAATCCCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCtt                                                                                                                     >  1:1044357/1‑135 (MQ=255)
             tGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCg                                                                                                                >  2:1246229/1‑139 (MQ=255)
                  cTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGaat                                                                                                                                 <  1:685963/117‑1 (MQ=255)
                  cTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGaat                                                                                                                                 >  2:685963/1‑117 (MQ=255)
                  cTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAaga                                                                                                           >  2:730861/1‑139 (MQ=255)
                    tCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAgaga                                                                                                         >  2:496668/1‑139 (MQ=255)
                     cAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGaa                                                                                                        <  2:644995/139‑1 (MQ=255)
                               tGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGaaaa                                                                                              >  2:1247569/1‑139 (MQ=255)
                                  aTCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTc                                                                                           <  1:730861/139‑1 (MQ=255)
                                  aTCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTc                                                                                           <  1:269218/139‑1 (MQ=255)
                                      tGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAaga                                                                                                           <  2:585156/119‑1 (MQ=255)
                                      tGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAaga                                                                                                           >  1:585156/1‑119 (MQ=255)
                                       gCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGcc                                                                                      <  1:84061/139‑1 (MQ=255)
                                       gCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGcc                                                                                      <  1:335828/139‑1 (MQ=255)
                                         tCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCtt                                                                                    <  1:1332110/139‑1 (MQ=255)
                                          cGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAACAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTa                                                                                   >  2:388666/1‑139 (MQ=255)
                                           gCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTAt                                                                                  <  1:496668/139‑1 (MQ=255)
                                           gCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTAACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTAt                                                                                  >  2:1028688/1‑139 (MQ=255)
                                              tGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTgg                                                                               <  1:847118/139‑1 (MQ=255)
                                                 tGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  >  1:53359/1‑85 (MQ=255)
                                                 tGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  <  2:53359/85‑1 (MQ=255)
                                                  gcgcAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGACGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  <  1:1330416/100‑1 (MQ=255)
                                                  gcgcAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGACGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  >  2:1330416/1‑100 (MQ=255)
                                                           aCTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGt                                                                  <  1:1247569/139‑1 (MQ=255)
                                                           aCTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGt                                                                  <  2:683261/139‑1 (MQ=255)
                                                           aCTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGt                                                                  <  2:354019/139‑1 (MQ=255)
                                                                  cGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCtt                                                                                                                     <  1:1358613/81‑1 (MQ=255)
                                                                  cGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCtt                                                                                                                     >  2:1358613/1‑81 (MQ=255)
                                                                  cGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCg                                                                                                                >  1:306016/1‑86 (MQ=255)
                                                                  cGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCg                                                                                                                <  2:306016/86‑1 (MQ=255)
                                                                         tGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTactga                                                    >  1:272921/1‑139 (MQ=255)
                                                                           cgcTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCaa                                                             >  1:304978/1‑128 (MQ=255)
                                                                           cgcTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCaa                                                             <  2:304978/128‑1 (MQ=255)
                                                                                     tataCTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGa                                        <  2:272921/139‑1 (MQ=255)
                                                                                        actgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTcg                                                                                          >  1:35806/1‑86 (MQ=255)
                                                                                        actgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTcg                                                                                          <  2:35806/86‑1 (MQ=255)
                                                                                          tgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  >  1:1218001/1‑44 (MQ=255)
                                                                                          tgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  <  2:1218001/44‑1 (MQ=255)
                                                                                          tgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATcc                                                                                                                            >  1:648373/1‑50 (MQ=255)
                                                                                          tgagactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATcc                                                                                                                            <  2:648373/50‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaat                                                                                                                                 <  2:920303/43‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaat                                                                                                                                 >  1:920303/1‑43 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  >  1:737194/1‑42 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  <  2:737194/42‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  <  1:293407/42‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGaa                                                                                                                                  >  2:293407/1‑42 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATc                                                                                                                             <  1:867928/47‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATc                                                                                                                             >  2:867928/1‑47 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  <  2:189356/58‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  <  1:347400/58‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  >  1:189356/1‑58 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  >  2:347400/1‑58 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCg                                                                                                                <  1:63/60‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCg                                                                                                                >  2:63/1‑60 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTAt                                                                                  >  2:1463733/1‑90 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTAt                                                                                  <  1:1463733/90‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCaa                                                             <  2:647194/111‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCaa                                                             >  1:647194/1‑111 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATg                                   <  2:1320907/137‑1 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATg                                   >  1:1320907/1‑137 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGa                                 >  1:104363/1‑139 (MQ=255)
                                                                                            agactgagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGa                                 >  1:540265/1‑139 (MQ=255)
                                                                                                 gagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  <  2:1423119/53‑1 (MQ=255)
                                                                                                 gagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTtgtg                                                                                                                  >  1:1423119/1‑53 (MQ=255)
                                                                                                 gagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGaa                                                                                                        >  2:1433685/1‑63 (MQ=255)
                                                                                                 gagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGaa                                                                                                        <  1:1433685/63‑1 (MQ=255)
                                                                                                 gagCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCa                            >  2:621008/1‑139 (MQ=255)
                                                                                                  agCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGAcc                                                                                                                       <  2:358577/47‑1 (MQ=255)
                                                                                                  agCGTCGATTCACCTGCAAACGGCGCATTCTTAGAATAATCCTGAcc                                                                                                                       >  1:358577/1‑47 (MQ=255)
                                                                                                                  cAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACCGACTGGTTGCCAGAATATGGa                                 >  2:388605/1‑117 (MQ=255)
                                                                                                                  cAAACGGCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACCGACTGGTTGCCAGAATATGGa                                 <  1:388605/117‑1 (MQ=255)
                                                                                                                       ggCGCATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGc      >  2:82636/1‑139 (MQ=255)
                                                                                                                         cgcATTCTTAGAATAGTCCTGACCTTGTGCGGAAGAGTAAACATGAAAATTCGCGCCTTATTGGTAGCAAt                                                                        >  2:1009691/1‑71 (MQ=255)
                                                                                                                         cgcATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAAt                                                                        <  1:1009691/71‑1 (MQ=255)
                                                                                                                          gcATTCTTAGAACAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGa   <  1:388666/139‑1 (MQ=255)
                                                                                                                           cATTCTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGaa  <  1:1489953/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                      
TGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAA  >  NC_000913/3081750‑3082011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: