Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,116,871	C→T	A748T (GCA→ACA)	sslE ←	putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,116,871	0	C	T	100.0%	188.6 / NA	58	A748T (GCA→ACA)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

CGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  >  NC_000913/3116737‑3117003                                                                                                                                       |                                                                                                                                     cGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCa                                                                                                                                  >  2:288007/1‑139 (MQ=255)         cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACCGACCTGTGGCATCCAACAg                                                                                                                          >  2:187305/1‑139 (MQ=255)         cACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAg                                                                                                                          >  2:86149/1‑139 (MQ=255)          aCCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGa                                                                                                                         <  1:288007/139‑1 (MQ=255)                         aTAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAc                                                                                                          <  2:1016212/139‑1 (MQ=255)                                           aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGa                                                                                        >  1:827432/1‑139 (MQ=255)                                                acGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCt                                                                                   >  2:942842/2‑139 (MQ=255)                                                  gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtca                                                                                 >  2:1384301/1‑139 (MQ=255)                                                    tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                               <  1:86149/139‑1 (MQ=255)                                                                      gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                             <  1:1384301/139‑1 (MQ=255)                                                                       cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                      >  2:739525/1‑129 (MQ=255)                                                                       cgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                      <  1:739525/129‑1 (MQ=255)                                                                                  cccTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                                 <  2:573258/139‑1 (MQ=255)                                                                                    cTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                               >  2:823743/1‑139 (MQ=255)                                                                                    cTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCACTCTCTTCCTTAAGATTGCtcat                                                                                <  2:790322/106‑1 (MQ=255)                                                                                    cTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCACTCTCTTCCTTAAGATTGCtcat                                                                                >  1:790322/1‑106 (MQ=255)                                                                                     ttGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTg                                              >  1:1173573/1‑139 (MQ=255)                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                  <  1:622187/86‑1 (MQ=255)                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                                  >  2:622187/1‑86 (MQ=255)                                                                                      tGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                             >  2:1412509/1‑139 (MQ=255)                                                                                        cGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                         <  2:1456090/77‑1 (MQ=255)                                                                                        cGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                         >  1:1456090/1‑77 (MQ=255)                                                                                         ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAc                                                                                                          <  1:598072/75‑1 (MQ=255)                                                                                         ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAc                                                                                                          >  2:598072/1‑75 (MQ=255)                                                                                         ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                                  >  1:59961/1‑131 (MQ=255)                                                                                         ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                                  <  2:59961/131‑1 (MQ=255)                                                                                         ggATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCa                                          <  1:823743/139‑1 (MQ=255)                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                      <  1:453499/97‑1 (MQ=255)                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcc                                                                      >  2:453499/1‑97 (MQ=255)                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                           <  2:913344/108‑1 (MQ=255)                                                                                                       tacCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCAc                                                           >  1:913344/1‑108 (MQ=255)                                                                                                        acCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcgc                                                                                                        >  2:373424/1‑62 (MQ=255)                                                                                                        acCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcgc                                                                                                        <  1:373424/62‑1 (MQ=255)                                                                                                        acCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                           >  2:1492810/1‑139 (MQ=255)                                                                                                        acCGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCGCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                           <  2:1498164/139‑1 (MQ=255)                                                                                                         ccGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATc                                                      >  2:566665/1‑111 (MQ=255)                                                                                                         ccGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATc                                                      <  1:566665/111‑1 (MQ=255)                                                                                                         ccGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                        <  1:1128826/125‑1 (MQ=255)                                                                                                         ccGACTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                        >  2:1128826/1‑125 (MQ=255)                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                       >  2:164654/1‑59 (MQ=255)                                                                                                            aCTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                       <  1:164654/59‑1 (MQ=255)                                                                                                             cTTGTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGACAGGCGATCAGATCGGTc                      <  1:942842/139‑1 (MQ=255)                                                                                                                gTTCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTcaca                   <  1:1412509/139‑1 (MQ=255)                                                                                                                 ttCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                      >  2:349603/1‑119 (MQ=255)                                                                                                                 ttCAGTGCCATCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                      <  1:349603/119‑1 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAAc                                                                                                               <  2:877919/36‑1 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAAc                                                                                                               >  1:877919/1‑36 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAc                                                                                                          <  2:462318/41‑1 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGAc                                                                                                          >  1:462318/1‑41 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                                 <  1:868931/98‑1 (MQ=255)                                                                                                                           tCGACAGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                                 >  2:868931/1‑98 (MQ=255)                                                                                                                                aGACCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCt   <  1:1492810/139‑1 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                       >  1:110263/1‑37 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                       <  2:110263/37‑1 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt                                                                           <  1:162400/65‑1 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt                                                                           >  2:162400/1‑65 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCtt  <  1:450909/138‑1 (MQ=255)                                                                                                                                  aCCTGTGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCtt  >  2:450909/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                     CGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTT  >  NC_000913/3116737‑3117003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑